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Team:Biwako Nagahama/Safety
From 2013.igem.org
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<h4>1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis | <h4>1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis | ||
organism, provide information on each of them:</h4> | organism, provide information on each of them:</h4> | ||
| - | <div> <h5>ORGANISMS USED:<h/5></div> | + | <div><h5>ORGANISMS USED:<h/5></div> |
a.We have used Escherichia coli K12 with KM109 strain without any disease risk for humans. | a.We have used Escherichia coli K12 with KM109 strain without any disease risk for humans. | ||
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c.The third type is Agrobacterium tumefadens C58 without any human disease risk. | c.The third type is Agrobacterium tumefadens C58 without any human disease risk. | ||
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All the used organisms belonged to the Risk Group 1. | All the used organisms belonged to the Risk Group 1. | ||
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<h4>2. Highest Risk Group Listed: 1</h4> | <h4>2. Highest Risk Group Listed: 1</h4> | ||
<h4>3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013</h4> | <h4>3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013</h4> | ||
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<h4>4.Do the biological materials used in your lab work pose any of the following risks?Please describe.</h4> | <h4>4.Do the biological materials used in your lab work pose any of the following risks?Please describe.</h4> | ||
| - | + | <h5>a.Risks to the safety and health of team members or others working in the lab?</h5> | |
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<div> | <div> | ||
No, all Escherichia coli strains have been used in molecular biology experiments.The Agrobacterium tumefaciens strain used in Our lab work has been used for transformation of plant cells,that was disarmed and showed no tumorigenicity on the plant,has no human toxicity. | No, all Escherichia coli strains have been used in molecular biology experiments.The Agrobacterium tumefaciens strain used in Our lab work has been used for transformation of plant cells,that was disarmed and showed no tumorigenicity on the plant,has no human toxicity. | ||
Revision as of 03:10, 27 September 2013
1. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
ORGANISMS USED:
b.The second type of organism used is Escherichia coli B strain (BL21(DE3) which do not have any disease risk for the humans.
c.The third type is Agrobacterium tumefadens C58 without any human disease risk.
All the used organisms belonged to the Risk Group 1.
2. Highest Risk Group Listed: 1
3. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013
a.Our team had BBa_K1044000 extracted from Agrobactterium tumefaclens C58.
b.Similarly, we had BBa_K1044001 had extracted from Agrobactterium tumefaclens C58.
c.We used BBa_K1044002 extraced from sperm whale by the 'Eurofins Scientific in Japan.'
4.Do the biological materials used in your lab work pose any of the following risks?Please describe.
a.Risks to the safety and health of team members or others working in the lab?
No, all Escherichia coli strains have been used in molecular biology experiments.The Agrobacterium tumefaciens strain used in Our lab work has been used for transformation of plant cells,that was disarmed and showed no tumorigenicity on the plant,has no human toxicity.
b.Risks to the safety and health of thc general public,if released by design or by accident?
No,all bacteria used in our lab work have no human toxicity.
c.Risks to thc environment,if released by design or by accident?
No, all bacteria used in our lab work have no ecological toxicity because of a‖ Escherichia coli strains have no ability in nature and the strain of Agrobacterium tumefaciens is disarmed.
5.If your project moved from a small-scalc lab study to become widely used as a commercial/industrial product,what new risks might arise?(Consider the different categories of risks that are listed in parts a‐ d of the previous question.)Also,what risks might arise if the knowledge you generate or the methods you develop became widely available?(Note:This is meant to be a somewhat open‐ ended discussion question.)
d.Risks to security through malicious misuse by individuals,groups,Or countries?
No.All bacteria used in our lab work have no human toxicity and all bacteria are stored in the freezer incubator that are not accessed by non‐ team members.
