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Team:Georgia State/protocols
From 2013.igem.org
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<h2>Protocols</h2> | <h2>Protocols</h2> | ||
<p class="description" style="text-align:left;"> | <p class="description" style="text-align:left;"> | ||
| - | Click on each button below to reveal the protocols we used for | + | Click on each button below to reveal the protocols we used for Mamba Juice. |
</p> | </p> | ||
<div class="makeclass" id="wrapper"> | <div class="makeclass" id="wrapper"> | ||
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<div class="accordionButton">Electroporation</div> | <div class="accordionButton">Electroporation</div> | ||
<div class="accordionContent"> | <div class="accordionContent"> | ||
| - | <img src="https://googledrive.com/host/0B4nfVqlTfnTzM0MwNkRQQUpuMzA" width="531" height="auto" /><img src="https://googledrive.com/host/ | + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzM0MwNkRQQUpuMzA" width="531" height="auto" /> |
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzOFJCbU8wdnBMQjg" width="531" height="auto" /> | ||
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</div> | </div> | ||
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<img src="https://googledrive.com/host/0B4nfVqlTfnTzcnQ2M2tZN2JFZk0" width="531" height="auto" /><br /> | <img src="https://googledrive.com/host/0B4nfVqlTfnTzcnQ2M2tZN2JFZk0" width="531" height="auto" /><br /> | ||
<img src="https://googledrive.com/host/0B4nfVqlTfnTzYzhvMlc1cWZlQlU" width="531" height="auto" /><br /> | <img src="https://googledrive.com/host/0B4nfVqlTfnTzYzhvMlc1cWZlQlU" width="531" height="auto" /><br /> | ||
| - | <img src="https://googledrive.com/host/ | + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzNlNCZlNZSzVIbDg" width="354" height="auto" /> |
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<div class="accordionButton">PCR</div> | <div class="accordionButton">PCR</div> | ||
<div class="accordionContent"> | <div class="accordionContent"> | ||
| - | <img src="https://googledrive.com/host/0B4nfVqlTfnTzY3NaTUI3aGZpREU" width="531" height="auto" />< | + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzY3NaTUI3aGZpREU" width="531" height="auto" /><img src="https://googledrive.com/host/0B4nfVqlTfnTzSmtYTU5WZ2cyVmc" width="531" height="auto" /> |
</div> | </div> | ||
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<div class="accordionButton">FastDigest</div> | <div class="accordionButton">FastDigest</div> | ||
<div class="accordionContent"> | <div class="accordionContent"> | ||
| - | <img src="https://googledrive.com/host/0B4nfVqlTfnTzdi1VLUJEU0dmaDQ" width="531" height="auto" />< | + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzdi1VLUJEU0dmaDQ" width="531" height="auto" /><img src="https://googledrive.com/host/0B4nfVqlTfnTzMVJfUXR3M0JwbTg" width="531" height="auto" /> |
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<div class="accordionContent"> | <div class="accordionContent"> | ||
<img src="https://googledrive.com/host/0B4nfVqlTfnTzTnRYM3ViTmJmV2M" width="531" height="auto" /><br /> | <img src="https://googledrive.com/host/0B4nfVqlTfnTzTnRYM3ViTmJmV2M" width="531" height="auto" /><br /> | ||
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzeFBPUlVwaVFxWGc" width="531" height="auto" /> | ||
</div> | </div> | ||
<div class="accordionButton">Ethanol Precipitation of DNA</div> | <div class="accordionButton">Ethanol Precipitation of DNA</div> | ||
<div class="accordionContent"> | <div class="accordionContent"> | ||
| - | <img src="https://googledrive.com/host/0B4nfVqlTfnTzUmZDV3NXaHhNWUE" width="531" height="auto" />< | + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzUmZDV3NXaHhNWUE" width="531" height="auto" /><img src="https://googledrive.com/host/0B4nfVqlTfnTzSE1uRGcxUEdBRW8" width="531" height="auto" /> |
</div> | </div> | ||
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<div class="accordionContent"> | <div class="accordionContent"> | ||
<img src="https://googledrive.com/host/0B4nfVqlTfnTzc2NucGdxNFlyNXM" width="531" height="auto" /><br /> | <img src="https://googledrive.com/host/0B4nfVqlTfnTzc2NucGdxNFlyNXM" width="531" height="auto" /><br /> | ||
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzVTJHYkk5aXJfSVE" width="531" height="auto" /><br /> | ||
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzRkpFZHdDcFh1c1E" width="531" height="auto" /> | ||
</div> | </div> | ||
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| + | <div class="accordionButton">Ligation of Insert DNA into Plasmid</div> | ||
| + | <div class="accordionContent"> | ||
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzNjJOdDFBMEdlSVE" width="531" height="auto" /> | ||
| + | <img src="https://googledrive.com/host/0B4nfVqlTfnTzcXh6Yll5ZFQ2Q0k" width="398" height="auto" /> | ||
| + | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 03:15, 27 September 2013
Protocols
Click on each button below to reveal the protocols we used for Mamba Juice.
Luria Plates
Preparation of E. Coli
Electroporation
MiniPrep
PCR
FastDigest
Heat Inactivation of Restriction Enzymes
Ethanol Precipitation of DNA
Gel Extraction of DNA
Ligation of Insert DNA into Plasmid
