Team:Washington/M9Media
From 2013.igem.org
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<b><u>M9 Media + Casamino Acid Recipe</u></b> | <b><u>M9 Media + Casamino Acid Recipe</u></b> | ||
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For 1L of media mix: </br></br> | For 1L of media mix: </br></br> | ||
- | 200 ml | + | 200 ml <a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></br> |
30 ml 10 mg/ml thiamine hydrochloride</br> | 30 ml 10 mg/ml thiamine hydrochloride</br> | ||
10 ml 40% glycerol </br> | 10 ml 40% glycerol </br> |
Latest revision as of 04:36, 27 September 2013
M9 Media + Casamino Acid Recipe For 1L of media mix: 200 ml 5X M9 Salts 30 ml 10 mg/ml thiamine hydrochloride 10 ml 40% glycerol 20 ml 10% casamino acids 20 ml 0.1M MgSO4 200 μl 0.5M CaCl2 dH2O until 1L Filter sterilize the mixture into sterile bottle.