Template:Kyoto/Notebook/Sep 27
From 2013.igem.org
(Difference between revisions)
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+ | ===RNA Extraction=== | ||
+ | |||
+ | {| class="wikitable" | ||
+ | !Name||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |9/26 Pcon-GFP-DT||423.0||1.83||1.46 | ||
+ | |- | ||
+ | |9/26 Pcon-GFP-DT K ||298.7||1.82||1.50 | ||
+ | |} | ||
+ | ===Electrophoresis=== | ||
+ | |||
+ | <!-- こっから --> | ||
+ | <div class="experiment"> | ||
+ | <span class="author">じっけんしゃ</span> | ||
+ | {| class="wikitable" | ||
+ | !Lane||Sample||Enzyme1||Enzyme2 | ||
+ | |- | ||
+ | |1||9/27 Pcon-GFP-DT(RNA) | ||
+ | |- | ||
+ | |2||1kb ladder | ||
+ | |- | ||
+ | |3||9/27 Pcon-GFP-DT K(RNA) | ||
+ | |} | ||
+ | [[File:igku_xxxxxx.xxx]]<br> |
Revision as of 05:40, 27 September 2013
Contents |
Sep 27
Gel Extraction
Lane | DNA | Enzyme |
---|---|---|
(>ω・)b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
(´ω`) | |||
(ΦωΦ^) |
Gel Extraction
Lane | DNA | Enzyme |
---|---|---|
(>ω・)b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
(´ω`) | |||
(ΦωΦ^) |
Electrophoresis
Gel Extraction
Lane | DNA | Enzyme |
---|---|---|
(>ω・)b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
(´ω`) | |||
(ΦωΦ^) |
Liquid Culture
Sample | medium |
---|---|
(・ω・) | |
(・X・) |
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
experiment | name | xx µL | name | xx µL | xx µL |
NC | name | xx µL | name | xx µL | xx µL |
Liquid Culture
Sample | medium |
---|---|
Pcon-spinach-DT | plusgrow Amp |
Pcon-tetRaptamer-DT | plusgrow Amp |
Pcon-antisense-spinach-DT | plusgrow Amp |
spinach-DT | plusgrow CP |
RNA Extraction
Name | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
9/26 Pcon-GFP-DT | 423.0 | 1.83 | 1.46 |
9/26 Pcon-GFP-DT K | 298.7 | 1.82 | 1.50 |
Electrophoresis
Lane | Sample | Enzyme1 | Enzyme2 |
---|---|---|---|
1 | 9/27 Pcon-GFP-DT(RNA) | ||
2 | 1kb ladder | ||
3 | 9/27 Pcon-GFP-DT K(RNA) |