Genovo is a Computer-Aided Design（CAD） tool to create genome denovo. The current version is 1.2. It consists of 12 plugins belonging to 3 design modules The first module, NeoChr, would assist users to grab related genes in different pathways manually, to rewire genes’ relationship logically, and to replace genes with ortholog that score higher. Then it would allow users to define gene order and orientation in DRAG&DROP way, and decouple the genes with overlap. In the end, it would add or delete features, such as telomere, loxp sites to build a brand new genome automatically. The second one , NucleoMod, would optimize the CDS regions and reduce their synthesis difficulty. And it helps design the CRIPSR site so that we can silence the wild type genes, and in the meanwhile, differentiate the wild type and synthesis type when assemling. This module also can erase or create enzyme site locally. The third one, SegmMan will cut chromosome into pieces with different sizes with Gibson, Goldengate, Homologous adaptors to them so that they are able to be assembled into whole experimentally. SegmMan, in another level, provide a complementary OLSDesign will guide users to get the chrosome fragments by chip and how to assembly them together. Genovo would enable users to create chromosome-like biodevice on their bent, and even futher genome research in pathway level.