Template:Kyoto/Notebook/Sep 27

From 2013.igem.org

(Difference between revisions)
(→Electrophoresis)
(→Gel Extraction)
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!Lane||DNA||Enzyme
!Lane||DNA||Enzyme
|-
|-
-
|（＞ω・）b || ||
+
|1||1kbp ladder||--
|-
|-
-
| || ||
+
|2||rowspan="3"|pSB1C3||rowspan="3"|EcoRI+SpeI
 +
|-
 +
|3
 +
|-
 +
|4
 +
|-
 +
|6||rowspan="3"|Pcon-attenuator-aptmer-DT||rowspan="3"|EcoRI+SpeI
 +
|-
 +
|7
 +
|-
 +
|8
 +
|-
 +
|10||rowspan="3"|Pcon-attenuator-aptmer-DT||rowspan="3"|EcoRI+SpeI
 +
|-
 +
|11
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|-
 +
|12
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Name||concentration[&micro;g/mL]||260/280||260/230
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|（´ω｀）|| || ||
+
|Pcon-attenuator-DT(EcoRI+SpeI)||6.8||1.98||0.43
 +
|-
 +
|pT181-attenuator(EcoRI+SpeI)||7.5||1.84||0.06
|-
|-
-
|（ΦωΦ^）|| || ||
+
|pSB1C3(EcoRI+SpeI)||17.7||1.97||0.71
|}
|}
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Revision as of 06:13, 27 September 2013

Contents

Sep 27

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
11kbp ladder--
2pSB1C3EcoRI+SpeI
3
4
6Pcon-attenuator-aptmer-DTEcoRI+SpeI
7
8
10Pcon-attenuator-aptmer-DTEcoRI+SpeI
11
12

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-attenuator-DT(EcoRI+SpeI)6.81.980.43
pT181-attenuator(EcoRI+SpeI)7.51.840.06
pSB1C3(EcoRI+SpeI)17.71.970.71

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Liquid Culture

No name

Samplemedium
(・ω・)
（・Ｘ・） 　

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µLnamexx µLxx µL
NCnamexx µLnamexx µLxx µL


Liquid Culture

No name

Samplemedium
Pcon-spinach-DTplusgrow Amp
Pcon-tetRaptamer-DTplusgrow Amp　
Pcon-antisense-spinach-DTplusgrow Amp
spinach-DTplusgrow CP

RNA Extraction

Nameconcentration[µg/mL]260/280260/230
9/26 Pcon-GFP-DT423.01.831.46
9/26 Pcon-GFP-DT K 298.71.821.50

Electrophoresis

じっけんしゃ

LaneSample
19/27 Pcon-GFP-DT(RNA)
21kb ladder
39/27 Pcon-GFP-DT K(RNA)

File:Igku xxxxxx.xxx

Miniprep

No name

DNA
Pcon-aptamer-DT
Pcon-spinach-DT
Pcon-antisense
Pcon-tetR-DT
Pcon-attenuator-DT
Pcon-antisense-spinach-DT

Transformation

No name

Name1Name2Sample1(µL)Sample2(µL)Competent Cells(µL)Total(µL)
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-attenuator-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-antisense-spinach-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-tetR aptamer-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-attenuator-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-antisense-spinach-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-tetR aptamer-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)--2--2022
Pcon-RBS-GFP-DT(4K5)--2--2022
attenuator(1C3)--2--2022
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