Team:Penn/Parts
From 2013.igem.org
(Difference between revisions)
Line 46: | Line 46: | ||
This protein de novo methylates CpG sites. It is derived from <i>Spiroplasma</i>, and functions by covalently bonding a methyl group (-CH<sub>3</sub>) to the cytosine residue within the site. CpG methylation, which normally ocurs in eukaryotes, is completely orthoganol to methylation by the DNA Adenine Methyltransferase enzyme found in <i>E.Coli,</i> which methylates GATC sites, and can be used for in vivo methylation studies in <i>E. coli.</i> It should be noted that CpG methylated DNA in <i>E. coli</i> is subject to Mcr and Mrr restriction, and should be used only in Mcr- and Mrr- <i>E. coli</i> strains. | This protein de novo methylates CpG sites. It is derived from <i>Spiroplasma</i>, and functions by covalently bonding a methyl group (-CH<sub>3</sub>) to the cytosine residue within the site. CpG methylation, which normally ocurs in eukaryotes, is completely orthoganol to methylation by the DNA Adenine Methyltransferase enzyme found in <i>E.Coli,</i> which methylates GATC sites, and can be used for in vivo methylation studies in <i>E. coli.</i> It should be noted that CpG methylated DNA in <i>E. coli</i> is subject to Mcr and Mrr restriction, and should be used only in Mcr- and Mrr- <i>E. coli</i> strains. | ||
</br> | </br> | ||
+ | </br></br> | ||
+ | <img src="https://www.dropbox.com/s/efg5zw06zgyabna/M.SssI.png" alt="some_text"> | ||
+ | </br></br> | ||
</br> | </br> | ||
<i><a href="http://parts.igem.org/Part:BBa_K1128002"><b>CpG Methylase M.SssI with Linker (BBa_K1128002)</b></a></i> | <i><a href="http://parts.igem.org/Part:BBa_K1128002"><b>CpG Methylase M.SssI with Linker (BBa_K1128002)</b></a></i> |
Revision as of 09:32, 27 September 2013
parts