Notebook

Dec.2012
IGEM-SCUT team was established. Every members brainstormed and presented their own ideas, then we discussed the feasibility of these ideas.

Dec.2012
IGEM-SCUT team was established. Every members brainstormed and presented their own ideas, then we discussed the feasibility of these ideas.

Jan.2013 to Feb.2013
After consulting our professors, the final project named E.cerevisiae was determined. Our team was divided into three parts, group of gas generating, odorant sensing and modeling. What gave us confidence was that our project was approved by the dean of the school of bioscience & bioengineering in our university (South China University of Technology). What’s more, we had a training camp at our school to learn some theoretical knowledges.

March.2013 to April.2013
Most of us were the first time to do experiments, so we had another training to improve our skills. And we have applied a lab for our project successfully.

April.2013 to present
Experiment started…

April.2013
WEEK 1
At the begining of our experiments,we got to make a plenty of preparation ,which included the preparation of the culture medium and the activation of bacterium .

WEEK 2
We still made some preparation for our experiments,in the meantime,we attended our classes.We were enthusiastic to the experiments although most of us had done few experiments.This week,we learned to prepare competent cells of Escherichia Coli (Top 10) and started to cultivating bacterium (Lactobacillus and BL21) using MRS and LB medium. We also did transformation experiments using plasmid and the newly-preparation Top-10 competent cellS.

WEEK 3
We made abstraction of the genome of Lactococcus lactis using the reagents that prepared by ourselves.Meanwhile,we transformed plasmid YEP352 into competent cells(E.coli).We also learned to prepare competent cells of BL21.To amplifiedα-Als gene,we did a PCR experiment.We did authentication of plasmid YEP352 by using enzyme E.coRⅠ.

May.2013
WEEK 1
We made preparation of YPD and FS medium that cultivated saccharomyces cerevisiaea and .Vibrio fischeri.We used electrophoresis to examinate the enzyme-digested plasmid.And we had constructed the system of diaceytl.What’s more,we cultivated the Vibrio fischeri into FS medium.

WEEK 2
We learned to do colony PCR to examine whether the target gene had transformed into the bacterium or not.We also used two kinds of restriction enzyme digestion to examine.Abstraction of the genome of Vibrio fischeri was done and the concentration of the genome was measured.