Team:USTC CHINA/Parts

From 2013.igem.org

(Difference between revisions)
Line 78: Line 78:
</div>
</div>
-
  <div class="part-col-1">
+
  <div class="part-col-2">
         <h1>PHT43</h1>
         <h1>PHT43</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
Line 84: Line 84:
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>HBsAg</h1>
         <h1>HBsAg</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
Line 90: Line 90:
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Ag85b</h1>
         <h1>Ag85b</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
Line 96: Line 96:
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Protective Antigen Domain 4</h1>
         <h1>Protective Antigen Domain 4</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>  
Line 102: Line 102:
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>LTB</h1>
         <h1>LTB</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
         <p>This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.</p>
         <p>This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Pctc+RBS+amilCP</h1>
         <h1>Pctc+RBS+amilCP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
         <p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). </p>
         <p>Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>SdpA,SdpB,SpbC</h1>
         <h1>SdpA,SdpB,SpbC</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
         <p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation.</p>
         <p>Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation.</p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>Pgrac+RBS+SDP</h1>
         <h1>Pgrac+RBS+SDP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
Line 123: Line 123:
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>PsdpRI+RBS+SDP</h1>
         <h1>PsdpRI+RBS+SDP</h1>
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
         <p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. </p>
         <p>We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC. </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
          
          
Line 139: Line 139:
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>promoter sdpR/I</h1>
         <h1>promoter sdpR/I</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
         <p>This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis.  </p>
         <p>This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis.  </p>
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
Line 154: Line 154:
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>RBS for Pgrac</h1>
         <h1>RBS for Pgrac</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
Line 160: Line 160:
</div>
</div>
-
<div class="part-col-1">
+
<div class="part-col-2">
         <h1>RBS for Pctc</h1>
         <h1>RBS for Pctc</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  

Revision as of 14:23, 27 September 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. Studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular material to reach systemic circulation.

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis.

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection.

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs).

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax.

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.

Pctc+RBS+amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response).

SdpA,SdpB,SpbC

BBa_K1074009

Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation.

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis.

PsdpRI+RBS+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.Killing is mediated by the exported toxic protein SpbC.

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG.

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis.

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N. Fuse it with the recombinant proteins to obtain the secretion of recombinant proteins in Bacillus subtilis.

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis. We used it in all of our gene circuits containing promoter Pgrac.

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis. It's also an universal RBS for promoters in Bacillus subtilis. We also used it with the promoter PsdpRI(BBa_K1074013) in our project.

Project

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Parts"