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Team:UTK-Knoxville/primers
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<ul class="DropDownMenu" id="MB1-DDM1"> | <ul class="DropDownMenu" id="MB1-DDM1"> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overall Project</span></span></a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material & Methods</span></span></a> |
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li> | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION1</span></span></a></li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION2</span></span></a></li> | ||
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<a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]--> | <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]--> | ||
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<ul class="SideMenu" id="MB8-DDM8-SM7"> | <ul class="SideMenu" id="MB8-DDM8-SM7"> | ||
| - | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/Aer"><span>Aer</span></a></li> |
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>HemAT</span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>HemAT</span></a></li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>RcoM</span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>RcoM</span></a></li> | ||
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<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(EnvZ)</span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(EnvZ)</span></a></li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(NtrC)</span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>(NtrC)</span></a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/primers"><span>Primers</span></a></li> |
</ul> | </ul> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Characterization</span></span></a></li> | ||
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| - | <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white"> | + | <a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon" style="color: white">Discussion<!--[if gt IE 6]><!--></a><!--<![endif]--> |
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</li> | </li> | ||
<li> | <li> | ||
| - | <a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <a href="https://2013.igem.org/Team:UTK-Knoxville/notebook" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]--> |
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| - | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span><span>SECTION1 ></span></span><!--[if gt IE 6]><!--></a><!--<![endif]--> |
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<ul class="SideMenu" id="MB1-DDM2-SM1"> | <ul class="SideMenu" id="MB1-DDM2-SM1"> | ||
| - | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/notebook"><span>SECTION2</span></a></li> |
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION3</span></a></li> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span>SECTION3</span></a></li> | ||
</ul> | </ul> | ||
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</li> | </li> | ||
<li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Visualization</span></span></a> | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Visualization</span></span></a> | ||
| - | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/ | + | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/methods"><span><span>Material & Methods</span></span></a> |
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a> | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Team Meetings</span></span></a> | ||
</li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a> | </li><li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Seminar on DETAILS</span></span></a> | ||
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| + | <a href="https://2013.igem.org/Team:UTK-Knoxville" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]--> | ||
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| + | <ul class="DropDownMenu" id="MB1-DDM6"> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Overview</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Advisors</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>Undergraduates</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>University</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION4</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION5</span></span></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:UTK-Knoxville/comingsoon"><span><span>SECTION6</span></span></a></li> | ||
| + | </ul> | ||
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| + | </li> | ||
<li> | <li> | ||
<a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a> | <a href="https://2013.igem.org/Team:UTK-Knoxville/sponsors" style="color: white">Sponsors</a> | ||
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<td>RcoM</td> | <td>RcoM</td> | ||
<td> </td> | <td> </td> | ||
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<p><br> | <p><br> | ||
| - | + | [<a href="https://2013.igem.org/Team:UTK-Knoxville" class="external text" rel="nofollow">back</a>] | |
</p> | </p> | ||
Revision as of 16:53, 26 June 2013
Primers
| Name | Sequence | Melting Temp.(°C) | Function/Comment | Status |
|---|---|---|---|---|
| General (Transmembrane) | ||||
| EnvZ_r | aaaaaactcgagttaggatccTTACCCTTCTTTTGTCGTGCC | 57 | Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc | In |
| OmpR_r | AAAAAACTCGAGAGGGGATCtcatgctttagagccgtccgg | 60 | Restriction sites: XhoI-ctcgag BamHI-ggatcc | In |
| OmpR_f | aaaaaaCATATGCAAGAGACCTACAAGATTCTG | 53 | Restriction site: NdeI-catatg | In |
| Aer | ||||
| Aer_f | aaaaaagaattctaaagatctaaggagatataATGTCTTCTCATCCGTATGTC | 53 | Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct | In 5/30 |
| Aer-EnvZ_1r | GCGCAAGTCGTGACTTACCCatttctgacactggacacctgg | 58 | Overlapping PCR primers | In 5/30 |
| Aer-EnvZ_1f | ccaggtgtccagtgtcagaaatGGGTAAGTCACGACTTGCGC | 59 | Overlapping PCR primers | In 5/30 |
| NarX | ||||
| NarX_F | We can order both sets of primers (transmembrane region from either protein) | |||
| NarX_R | ||||
| Nar-EnvZ_R | ||||
| HemAT | ||||
| HemAT_F | aaaaaagaattctaaagatctaaggagatataTTGTTATTTAAAAAAGACAGAAAACAAG | 52 | Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct | Ordered 6/12 |
| HemAT_R | ggcgctggttatccatcggactggttgtactcgctttgaaacg | 58 | Overlapping PCR primers | Ordered 6/12 |
| HemAT_NtrB_F | cgtttcaaagcgagtacaaccagtccgatggataaccagcgcc | 61 | Overlapping PCR primers | Ordered 6/12 |
| TodS | ||||
| RcoM | ||||
| RcoM_F | aaaaaagaattctaaagatctaaggagatataTTAGTTTTTACTTGCTTCAGTTGG | 54 | Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct | Ordered 6/12 |
| RcoM_R | ggcgctggttatccatcggaatgtttaaatattttgcaattgcttc | 52 | Overlapping PCR primers | Ordered 6/12 |
| RcoM_NtrB_F | gaagcaattgcaaaatatttaaacattccgatggataaccagcgcc | 61 | Overlapping PCR primers | Ordered 6/12 |
| LasR | ||||
| Cytoplasmic Construct | ||||
| NtrC_F | aaaaaacatatgatgcaacgagggatagtctgg | 58 | Restriction site: NdeI-catatg | Ordered 6/12 |
| NtrC_R | agatccgagctggggatggagtgactcgagaaaaaa | 57 | XhoI-ctcgag | Ordered 6/12 |
| NtrB_R | aaaaaactcgagttaggatccTTATTTCCTGATAGGCAGGTAAAC | 54 | Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc | Ordered 6/12 |
| The backbone will be cut with NedI on the OmpR and NtrC side and BamHI on the EnvZ and NtrB side |
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