Team:Osaka/Achievement

From 2013.igem.org

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<p class="aaaa2">Although we did not encounter any issues with biosafety during the course of this project, a discussion of safety is mandated by the iGEM requirements so we included it here.</p>
 
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Q1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety or environmental safety ?</p>
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Our Achievements
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No, our project ideas do not raise any safety issue in terms of researcher safety, public safety or environmental safety. We use E.coli(K12) strain DH5α, DH10b, DH1, and DNA used in our laboratory were all of safe genes, moreover, our experiment was controlled under P1 level room. They are safe for the researcher and the environment, and they do not have any hazard outside the lab.</p>
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Using the iGEM 2013 Judging Form as a guide, we summarize our achievements below.
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・Requirement for a Bronze medal:
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Q2. Can your project auses risks to security through malicious misuse by individuals, groups, or countries?</p>
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1, Register the team, have a great summer, and plan to have fun at the Jamboree.
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→We had a great summer, and are looking forward to having fun at the Jamboree!!
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No, within the compass of our imagination, our project ideas cannot be used for some malicious misuse.</p>
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2, Successfully complete and submit the iGEM 2013 Judging form.
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→Submitted!
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Q3, If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise?</p>
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3, Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.
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→Done!
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Our project idea contains drug resistance gene. So if our project ideas widely used in society, they can accelerate the evolution of the drug-resistant bacterium.</p>
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4, Plan to present a Poster and Talk at the iGEM Jamboree.
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→Design of the poster is underway; the members are already practicing their presentations.
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Q4, Does your project include any design features to address safety risks?</p>
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５, Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts.
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5-1, Description of function
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5-2, Quantitative data showing the Part or Device function
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In our project, we use –ilvE E.coli strain which cannot produce isoleucine. In addition to that, we have construct apart that codes ilvE in biobrick format. They canbe used to construct an auxotrophic chassis system to prevent bio-hazard.</p>
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5-3, Acknowedgment of sources and references
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→We have submitted 8 parts (BBa_K1040005~K1040013) to the Registry, shown its references, and see quantitative data showing its function ( BBa_K1040005 )
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Q5, What safety training have you received?</p>
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・Additional Requirements for a Silver Medal:
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１, Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.
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1-1, Description of function
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Our faculty adviser Prof. Kakimoto gave us safety training. It was about how we safely conduct experiments in a lab, and how we treat and dispose genetically engineered orginisms to prevent bio-hazard.</p>
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1-2, Quantitative data showing the Part or Device function
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1-3, Acknowedgment of sources and references
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→We demonstrated that E.coli, ilvE gene improves E.coli’s(-ilvE strain) growth in M63 medium. See documentation at Registry or the Project-Result page.
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Q6, Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.</p>
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Additional Requirements for a Gold Medal: (one OR more)
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Yes, our university has a biosafety committee which requires students and faculty alike to take a biosafety course as well as submit an application before engaging in any research activity involving genetically-modified organisms. In general, as we stuck to the 'safe' organisms of E. coli , the committee did not show any objection to our project. Here is the link to biosafety guidelines of our institution. ( http://www.osaka-u.ac.jp/jp/about/kitei/reiki_honbun/u035RG00000478.html )</p>
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→We produced “E.col-interior” as our human practice. To have ordinary people feel familiar with synthetic biology, it is important to tell them what is E.coli and how fantastic they are, because E.coli has been playing important role in many aspect of synthetic biology. Therefore, our project contribute to make E.coli and synthetic biology familiar to general public!</p>
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Revision as of 18:02, 27 September 2013

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Our Achievements Using the iGEM 2013 Judging Form as a guide, we summarize our achievements below. ・Requirement for a Bronze medal: 1, Register the team, have a great summer, and plan to have fun at the Jamboree. →We had a great summer, and are looking forward to having fun at the Jamboree!! 2, Successfully complete and submit the iGEM 2013 Judging form. →Submitted! 3, Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts. →Done! 4, Plan to present a Poster and Talk at the iGEM Jamboree. →Design of the poster is underway; the members are already practicing their presentations. ５, Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). A new application of, and outstanding documentation (quantitative data showing the Part's/ Device's function), of a previously existing BioBrick part in the 'Experience' page of that part's Registry entry also counts. 5-1, Description of function 5-2, Quantitative data showing the Part or Device function 5-3, Acknowedgment of sources and references →We have submitted 8 parts (BBa_K1040005~K1040013) to the Registry, shown its references, and see quantitative data showing its function ( BBa_K1040005 ) ・Additional Requirements for a Silver Medal: １, Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. 1-1, Description of function 1-2, Quantitative data showing the Part or Device function 1-3, Acknowedgment of sources and references →We demonstrated that E.coli, ilvE gene improves E.coli’s(-ilvE strain) growth in M63 medium. See documentation at Registry or the Project-Result page. Additional Requirements for a Gold Medal: (one OR more) →We produced “E.col-interior” as our human practice. To have ordinary people feel familiar with synthetic biology, it is important to tell them what is E.coli and how fantastic they are, because E.coli has been playing important role in many aspect of synthetic biology. Therefore, our project contribute to make E.coli and synthetic biology familiar to general public!

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