Team:UCSF/lily2

From 2013.igem.org

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<!--Main Head from Calgary 2012-->
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{{Template:UCSF/MainHeader}}
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{{Template:UCSF/SlideShow}}
<html>
<html>
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<!--
 
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Currently unable to implement the CSS switching due to the stupidity of the wiki. Will work on other things instead.
 
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New header structure
 
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-->
 
<head>
<head>
<!--CSS styles: global-->
<!--CSS styles: global-->
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<!--<link rel='stylesheet' type='text/css' href="https://2012.igem.org/Team:Calgary/static/basicpageglobal.css?action=raw&ctype=text/css" />-->
 
<style type="text/css">
<style type="text/css">
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    /***
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/***
Minimal header: removes the search bar and header image and readjusts font colours in the menus.
Minimal header: removes the search bar and header image and readjusts font colours in the menus.
-
Thanks a lot to the 2011 Brown-Stanford, 2012 Lethbridge iGEM and 2012 Calgary teams for snippets of their code!
+
Thanks a lot to the 2011 Brown-Stanford and 2012 Lethbridge iGEM teams for snippets of their code!
Check out their wikis at:
Check out their wikis at:
https://2011.igem.org/Team:Brown-Stanford
https://2011.igem.org/Team:Brown-Stanford
https://2012.igem.org/Team:Lethbridge
https://2012.igem.org/Team:Lethbridge
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https://2012.igem.org/Team:Calgary
 
***/
***/
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border: none;
border: none;
}
}
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h1, h2, h3, h4, #css-full, #css-mobi{
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h1, h2,h3, h4, #css-full, #css-mobi{
font-family: Myriad Pro, Gill Sans MT, Trebuchet MS, Arial, Sans-Serif;
font-family: Myriad Pro, Gill Sans MT, Trebuchet MS, Arial, Sans-Serif;
border: 0;
border: 0;
font-weight: 400;
font-weight: 400;
}
}
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p, div.thumb div div.thumbcaption{
p, div.thumb div div.thumbcaption{
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font-family: Georgia, Serif;
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font-family: Calibri, Sans-Serif;
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font-size: 1.1em;
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font-weight: normal;
font-weight: normal;
color: black;
color: black;
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margin: 10px;
margin: 10px;
}
}
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</style>
 
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<!--desktop-->
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#textlink a:link,
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<!--<link rel='stylesheet' type='text/css' href='https://2012.igem.org/Team:Calgary/static/basicpagedesktop.css?action=raw&ctype=text/css' />-->
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a:visited{
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<style type="text/css">
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font-family: Calibri, Sans-Serif;
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    /*======
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font-weight: normal;
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Desktop Styling
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color: #008000;
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Thanks a lot to the 2012 Calgary team for snippets of their code!
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text-decoration:none;  
-
Check out their wiki at:
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-
https://2012.igem.org/Team:Calgary
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-
 
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-
======*/
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-
 
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-
/***Nav styling***/
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header{
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position: relative;
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top: -45px;
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z-index: 999;
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}
}
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#nav-wrap{
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#textlink a:hover,
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height: 0px;
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a:active{
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margin-top: -45px;
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font-family: Calibri, Sans-Serif;
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font-weight: normal;
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color: #008000;
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text-decoration:underline;
}
}
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#nav, #nav ul{
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/*======
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list-style: none;
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Desktop Styling
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margin: 0;
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======*/
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padding: 0;
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width: 965px;
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height: 100%;
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display: table;
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-
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-
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}
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/*To be moved to JQ block*/
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display: none;
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}
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-
 
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/*menu*/
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#nav li{
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height: auto;
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padding: 0;
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list-style: none;
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float: left;
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width: auto;
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margin: 0;
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background: #333333;
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position: relative;
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}
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#nav > li a{
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padding: 0 15px;
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}
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#nav > li{
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background: transparent;
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}
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-
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/*submenu*/
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#nav li ul {
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position: absolute;
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width: 200px;
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display: none;
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}
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#nav li:hover ul {
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display: block;
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/*sub-submenu*/
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#nav li ul ul{
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margin-left: 230px;
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margin-top: -15px;
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-
}
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display: none;
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#nav li:hover ul, #nav li li:hover ul{
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#nav a{
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display: block;
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font-family: Myriad Pro, Gill Sans MT, Trebuchet MS, Arial, Sans-Serif;
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color: white;
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#nav li a{
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line-height: 1.4em; /*centers the text vertically*/
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font-size: 2em;
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}
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#nav ul li > a{
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width: 200px;
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}
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/*color change after rollover*/
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#nav li a:hover, #nav li li a.drop:hover::after{
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display: block;
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text-decoration: none;
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color: #43bbff;
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}
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#nav li ul li ul{
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margin-top: -32px;
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position: absolute;
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}
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/*submenu and sub-submenu*/
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#nav li ul li ul li a, #nav li ul li a{
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font-size: 1.8em;
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#nav li li a.drop:after{
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content: '>';
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padding-left: 20px;
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color: #BBB;
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display: inline;
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float: right;
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}
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-
 
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/***End nav styling***/
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-
 
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/***Headerimage***/
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#headerimage{
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width: 968px;
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position: relative;
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margin-left: 0px;
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margin-bottom: 10px;
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top: 0px;
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}
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#css-full{
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#css-mobi{
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}
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/***Logo styling***/
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#logo{
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position: absolute;
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top: 10px;
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right: 20px;
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float: right;
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}
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#logo img{
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width: 260px;
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}
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/***Body styling***/
/***Body styling***/
h1{
h1{
-
font-size: 2.3em;
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font-size: 2.5em;
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line-height: 1em;
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}
}
h2{
h2{
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font-size: 1.8em;
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font-size: 1.7em;
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line-height: 1.2em;
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}
}
h3{
h3{
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font-size: 1.6em;
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font-size: 1.7em;
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margin: 0px 15px;
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font-weight: bold;
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}
}
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h4{
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font-size: 1.4em;
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#box1{
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color: #333333;
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width: 980px;
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margin: 0px 20px;
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margin-top: -8px;
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font-weight: bold;
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background: #fffff;
-
}
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float: left;
-
#bodycontainer p{
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-
font-size: 1.2em;
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-
}
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#pagetitle{
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border-bottom: 2px solid black;
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padding-bottom: 10px;
padding-bottom: 10px;
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padding-left: 10px;
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margin-bottom: 5px;
}
}
-
#bodycontainer h2{
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#box1 h2{
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margin-left: 10px;
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}
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margin-right: 10px;
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#box1 p{
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}
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font-size: 1.25em;
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#bodycontainer p{
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-
margin-left: 20px;
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margin-right: 10px;
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-
}
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#bodycontainer{
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margin-left: 220px;
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-
}
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#bodycontainer ul{
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margin-left: 5.0em;
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-
}
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-
#bodycontainer li{
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font-family: Georgia, Serif;
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-
}
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-
#sidebar{
+
-
position: absolute;
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-
width: 210px;
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-
z-index: 0;
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-
}
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-
/*
+
-
#sidebarimg{
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width: 214px;
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height: 144px;
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background: #43bbff;
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-
margin: 5px 0px;
+
-
border: 3px solid #333333;
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}
}
-
*/
 
-
#sidebar #list{
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#UCSFLogo{
-
background: #333333;
+
-
}
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-
#sidebar h2{
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-
color: white;
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-
padding: 20px 15px 0px 15px;
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-
font-size: 2.0em;
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-
}
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-
#sidebar ul{
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-
list-style: none;
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-
margin: 0px 15px;
+
-
}
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-
#sidebar #list > ul{
+
-
padding-bottom: 20px;
+
-
}
+
-
#sidebar a{
+
-
color: white;
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-
font-family: Georgia, Serif;
+
-
font-size: 1.4em;
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display: block;
display: block;
-
line-height: 1.4em;
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float: left;
 +
width: 250px;
 +
height:200px;
 +
background: url('https://static.igem.org/mediawiki/2013/6/6b/UCSF2013_Logonew.png');
 +
background-position: top;
 +
margin: 40px;
}
}
-
#sidebar a:hover{
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-
text-decoration: none;
+
/*Floatbox styling*/
-
color: #43BBFF;
+
.floatbox
-
}
+
              {
 +
                    width:980px;
 +
                    float:left;
 +
                    background-color: #fffff;
 +
                    margin-top: 10px;
 +
                    padding-bottom: 10px;
 +
                    margin-bottom: 5px;
 +
                    text-align:justify;
 +
              }
 +
/*Showbox Styling*/
-
/* thumbnails */
+
      .showbox tr:first-child td {  
-
div.thumb {
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          border-top: 0px;
-
    margin-bottom: .5em;
+
          padding-top: 1px
-
    border-style: solid;
+
      }
-
    border-color: transparent;
+
      .showbox tr:last-child td {
-
    width: auto;
+
          border-bottom: 0px;
-
}
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          padding-bottom: 1px
-
div.thumb div {
+
      }
-
    border: 0px;
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      .showbox tr td:first-child {
-
    padding: 3px !important;
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          border-left: 0px;
-
    font-size: 94%;
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          padding-left: 1px;
-
    text-align: center;
+
      }
-
    overflow: hidden;
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      .showbox tr td:last-child {
-
background: transparent;
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          border-right: 0px;
-
}
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          padding-right: 1px;
-
div.thumb div a img {
+
      }
-
    border: none;
+
-
}
+
-
div.thumb div div.thumbcaption {
+
-
    border: none;
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-
    text-align: left;
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-
    line-height: 1.4em;
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-
    padding: .3em 0 .1em 0;
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-
+
-
}
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-
div.magnify {
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-
    float: right;
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    border: none !important;
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-
    background: none !important;
+
-
}
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-
div.magnify a, div.magnify img {
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-
    display: block;
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-
    border: none !important;
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-
    background: none !important;
+
-
}
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-
div.tright {
+
-
    clear: right;
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-
    float: right;
+
-
    border-width: .5em 0 .8em 1.4em;
+
-
}
+
-
div.tleft {
+
-
    float: left;
+
-
    margin-right: .5em;
+
-
    border-width: .5em 1.4em .8em 0;
+
-
}
+
-
/*colouring*/
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      .showbox {
-
/*oranges: current page and all sidebar rollovers*/
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          margin-top: -18px;   
-
#home header #homelink, #home #sidebar #list a:hover, #home #nav li a:hover, #home #nav li a.drop:hover::after,
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          background:none;
-
#team header #teamlink, #team #sidebar #list a:hover, #team #nav li a:hover, #team #nav li a.drop:hover::after,
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          color:#ffffff;
-
#proj_hp header #projectlink, #proj_hp #sidebar #list a:hover, #proj_hp #nav li a:hover, #proj_hp #nav li a.drop:hover::after,
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          vertical-align:baseline;
-
#parts header #partslink, #parts #sidebar #list a:hover, #parts #nav li a:hover, #parts #nav li a.drop:hover::after,
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          border-collapse:collapse;
-
#notebook header #notebooklink, #notebook #sidebar #list a:hover, #notebook #nav li a:hover, #notebook #nav li a.drop:hover::after,
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          }
-
#outreach header #outreachlink, #outreach #sidebar #list a:hover, #outreach #nav li a:hover, #outreach #nav li a.drop:hover::after,
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      .showbox td {
-
#sponsors header #sponsorslink, #sponsors #sidebar #list a:hover, #sponsors #nav li a:hover, #sponsors #nav li a.drop:hover::after{
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          margin-top: -18px;         
-
color: #F6A82D;
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          background:none;
-
}
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          border:none;
-
/*oranges: sidebar border*/
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          padding:10px;
-
#home #sidebar #list,
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          width:50%;
-
#team #sidebar #list,
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          }
-
#proj_hp #sidebar #list,
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      .showbox p {
-
#parts #sidebar #list,
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          text-align:top;
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#notebook #sidebar #list,
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          color:black;
-
#outreach #sidebar #list,
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          margin:0px;
-
#sponsors #sidebar #list{
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          font-size: 1.25em;
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border-left: 10px solid #F6A82D;
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          }
-
}
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      .showbox img {
-
/*oranges: links*/
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          height:150px;
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#team #bodycontainer a,
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          width:180px;
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#proj_hp #bodycontainer a,
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          float:left;
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#parts #bodycontainer a,
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          border-radius:4px;
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#notebook #bodycontainer a,
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          margin-right:10px;
-
#outreach #bodycontainer a,
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          }
-
#sponsors #bodycontainer a{
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      a:hover {
-
color: #FF7A00;
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          text-decoration:none;
-
}
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          }
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#team #bodycontainer a:visited,
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-
#proj_hp #bodycontainer a:visited,
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-
#parts #bodycontainer a:visited,
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-
#notebook #bodycontainer a:visited,
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-
#outreach #bodycontainer a:visited,
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-
#sponsors #bodycontainer a:visited{
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-
color: #C40;
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-
}
+
-
/*blues: current page, all rollover links in nav, all sidebar hovers and dropdown menus*/
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  /*end showboxstyling */
-
#proj_o header #projectlink, #proj_o #sidebar #list a:hover, #proj_o #nav li a:hover, #proj_o #nav li a.drop:hover::after,
+
-
#note_o header #notebooklink, #note_o #sidebar #list a:hover, #note_o #nav li a:hover, #note_o #nav li a.drop:hover::after{
+
-
color: #43BBFF;
+
-
}
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-
/*blues: sidebar border*/
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#proj_o #sidebar #list,
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#note_o #sidebar #list{
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border-left: 10px solid #43BBFF;
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-
}
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-
/*blues: links*/
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#proj_o #bodycontainer a,
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#note_o #bodycontainer a{
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-
color: #1088CC;
+
-
}
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-
#proj_o #bodycontainer a:visited,
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-
#note_o #bodycontainer a:visited{
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color: #05B;
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-
}
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-
/*greens: current page, all rollover links in nav, all sidebar hovers, and dropdown menus*/
 
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#proj_f header #projectlink, #proj_f #sidebar #list a:hover, #proj_f #nav li a:hover, #proj_f #nav li a.drop:hover::after,
 
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#note_f header #notebooklink, #note_f #sidebar #list a:hover, #note_f #nav li a:hover, #note_f #nav li a.drop:hover::after{
 
-
color: #58CD45;
 
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}
 
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/*greens: sidebar border*/
 
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#proj_f #sidebar #list,
 
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#note_f #sidebar #list{
 
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border-left: 10px solid #58CD45;
 
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}
 
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/*greens: links*/
 
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#proj_f #bodycontainer a,
 
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#proj_f #bodycontainer a{
 
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color: #159900;
 
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}
 
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/*greens: links*/
 
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#proj_f #bodycontainer a:visited,
 
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#proj_f #bodycontainer a:visited{
 
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color: #06660B;
 
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}
 
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/*hub page CSS*/
 
-
a.hublink{
 
-
text-decoration: none;
 
-
margin-left: 15px;
 
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margin-right: 15px;
 
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}
 
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div.hubbox{
 
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margin-left: 15px;
 
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}
 
-
div.hubbox img{
 
-
float: left;
 
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padding: 15px;
 
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}
 
-
div.hubbox h2{
 
-
color: white;
 
-
padding: 15px 15px 0px 15px;
 
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font-size: 2.0em;
 
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margin-left: 120px !important;
 
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}
 
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div.hubbox p{
 
-
color: white;
 
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padding: 0px 15px 15px 15px;
 
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margin-left: 120px !important;
 
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}
 
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div.hubbox b{
 
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font-size: 1.1em;
 
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}
 
</style>
</style>
-
 
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<script type="text/javascript">
 
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    jQuery(document).ready(function ($) {
 
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        //eliminate jsnotice
 
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        $('#jsnotice').hide();
 
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        //prepend menu icon
 
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        $('#nav-wrap').prepend('<div id="menu-icon">Menu</div>');
 
-
 
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        //toggle nav
 
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        $("#menu-icon").click(function () {
 
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            $("#nav").slideToggle('fast');
 
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            $(this).toggleClass("active");
 
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        });
 
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        //hide url bar
 
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        window.scrollTo(0, 1);
 
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    });
 
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</script>
 
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<!--switching function: thanks to http://www.digital-web.com/articles/strategies_for_css_switching/-->
 
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<script type="text/javascript">
 
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    var _gaq = _gaq || [];
 
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    _gaq.push(['_setAccount', 'UA-32774032-1']);
 
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-
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-
 
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</script>
 
-
 
</head>
</head>
<body>
<body>
 +
<!--Abstract-->
 +
<div id="box1" align="justify">
 +
<a id="UCSFLogo">
 +
</a>
 +
<h2><center>Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome</h2></center>
 +
<p>In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. The goal of our project is to take advantage of a natural horizontal gene transfer mechanism in bacteria to precisely affect gene expression in selected strains. We combine bacterial conjugation with CRISPRi, an RNAi-like repression system developed from bacteria, to regulate gene expression in targeted strains within a complex microbial community. One possible application is to selectively repress pathogenic genes in a microbiome, leaving the community makeup unaffected. In addition, we use CRISPRi to lay the groundwork for transferring large circuits that enable complex functionality and decision-making in cells. </p>
-
<header>
+
</div>
-
<!--
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                    <a href="https://2013.igem.org/Team:UCSF/About_Us"><h3>Team</h3></a>
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                    <p>Meet our dedicated team of under-graduates who conceived of, planned, and carried out the whole project.</p></a>
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                    <p>Discover our precision guided tools to target unique species in a complex microbiome and our groundwork for transferring large circuits that enable complex functionality and decision-making in cells.</p>           
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(p = n.split("."), n = p.shift(), j.namespace = p.slice(0).sort().join(".")) : (p = [], j.namespace = ""), j.type = n, j.guid || (j.guid = f.guid); var q = l[n], r = d.event.special[n] || {}; if (!q) { q = l[n] = []; if (!r.setup || r.setup.call(c, g, p, m) === !1) c.addEventListener ? c.addEventListener(n, m, !1) : c.attachEvent && c.attachEvent("on" + n, m) } r.add && (r.add.call(c, j), j.handler.guid || (j.handler.guid = f.guid)), q.push(j), d.event.global[n] = !0 } c = null } }, global: {}, remove: function (a, c, e, f) { if (a.nodeType !== 3 && a.nodeType !== 8) { e === !1 && (e = v); var g, h, i, j, k = 0, l, m, n, o, p, q, r, s = d.hasData(a) && d._data(a), t = s && s.events; if (!s || !t) return; c && c.type && (e = c.handler, c = c.type); if (!c || typeof c === "string" && c.charAt(0) === ".") { c = c || ""; for (h in t) d.event.remove(a, h + c); return } c = c.split(" "); while (h = c[k++]) { r = h, q = null, l = h.indexOf(".") < 0, m = [], l || (m = h.split("."), h = m.shift(), n = new RegExp("(^|\\.)" + d.map(m.slice(0).sort(), u).join("\\.(?:.*\\.)?") + "(\\.|$)")), p = t[h]; if (!p) continue; if (!e) { for (j = 0; j < p.length; j++) { q = p[j]; if (l || n.test(q.namespace)) d.event.remove(a, r, q.handler, j), p.splice(j--, 1) } continue } o = d.event.special[h] || {}; for (j = f || 0; j < p.length; j++) { q = p[j]; if (e.guid === q.guid) { if (l || n.test(q.namespace)) f == null && p.splice(j--, 1), o.remove && o.remove.call(a, q); if (f != null) break } } if (p.length === 0 || f != null && p.length === 1) (!o.teardown || o.teardown.call(a, m) === !1) && d.removeEvent(a, h, s.handle), g = null, delete t[h] } if (d.isEmptyObject(t)) { var w = s.handle; w && (w.elem = null), delete s.events, delete s.handle, d.isEmptyObject(s) && d.removeData(a, b, !0) } } }, trigger: function (a, c, e) { var f = a.type || a, g = arguments[3]; if (!g) { a = typeof a === "object" ? a[d.expando] ? a : d.extend(d.Event(f), a) : d.Event(f), f.indexOf("!") >= 0 && (a.type = f = f.slice(0, -1), a.exclusive = !0), e || (a.stopPropagation(), d.event.global[f] && d.each(d.cache, function () { var b = d.expando, e = this[b]; e && e.events && e.events[f] && d.event.trigger(a, c, e.handle.elem) })); if (!e || e.nodeType === 3 || e.nodeType === 8) return b; a.result = b, a.target = e, c = d.makeArray(c), c.unshift(a) } a.currentTarget = e; var h = d._data(e, "handle"); h && h.apply(e, c); var i = e.parentNode || e.ownerDocument; try { e && e.nodeName && d.noData[e.nodeName.toLowerCase()] || e["on" + f] && e["on" + f].apply(e, c) === !1 && (a.result = !1, a.preventDefault()) } catch (j) { } if (!a.isPropagationStopped() && i) d.event.trigger(a, c, i, !0); else if (!a.isDefaultPrevented()) { var k, l = a.target, m = f.replace(p, ""), n = d.nodeName(l, "a") && m === "click", o = d.event.special[m] || {}; if ((!o._default || o._default.call(e, a) === !1) && !n && !(l && l.nodeName && d.noData[l.nodeName.toLowerCase()])) { try { l[m] && (k = l["on" + m], k && (l["on" + m] = null), d.event.triggered = !0, l[m]()) } catch (q) { } k && (l["on" + m] = k), d.event.triggered = !1 } } }, handle: function (c) { var e, f, g, h, i, j = [], k = d.makeArray(arguments); c = k[0] = d.event.fix(c || a.event), c.currentTarget = this, e = c.type.indexOf(".") < 0 && !c.exclusive, e || (g = c.type.split("."), c.type = g.shift(), j = g.slice(0).sort(), h = new RegExp("(^|\\.)" + j.join("\\.(?:.*\\.)?") + "(\\.|$)")), c.namespace = c.namespace || j.join("."), i = d._data(this, "events"), f = (i || {})[c.type]; if (i && f) { f = f.slice(0); for (var l = 0, m = f.length; l < m; l++) { var n = f[l]; if (e || h.test(n.namespace)) { c.handler = n.handler, c.data = n.data, c.handleObj = n; var o = n.handler.apply(this, k); o !== b && (c.result = o, o === !1 && (c.preventDefault(), c.stopPropagation())); if (c.isImmediatePropagationStopped()) break } } } return c.result }, props: "altKey attrChange attrName bubbles button cancelable charCode clientX clientY ctrlKey currentTarget data detail eventPhase fromElement handler keyCode layerX layerY metaKey newValue offsetX offsetY pageX pageY prevValue relatedNode relatedTarget screenX screenY shiftKey srcElement target toElement view wheelDelta which".split(" "), fix: function (a) { if (a[d.expando]) return a; var e = a; a = d.Event(e); for (var f = this.props.length, g; f; ) g = this.props[--f], a[g] = e[g]; a.target || (a.target = a.srcElement || c), a.target.nodeType === 3 && (a.target = a.target.parentNode), !a.relatedTarget && a.fromElement && (a.relatedTarget = a.fromElement === a.target ? 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function (a, b, c) { a.removeEventListener && a.removeEventListener(b, c, !1) } : function (a, b, c) { a.detachEvent && a.detachEvent("on" + b, c) }, d.Event = function (a) { if (!this.preventDefault) return new d.Event(a); a && a.type ? 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[e] : b : [] } }, l.filter.ID = function (a, b) { var c = typeof a.getAttributeNode !== "undefined" && a.getAttributeNode("id"); return a.nodeType === 1 && c && c.nodeValue === b }), e.removeChild(a), e = a = null } (), function () { var a = c.createElement("div"); a.appendChild(c.createComment("")), a.getElementsByTagName("*").length > 0 && (l.find.TAG = function (a, b) { var c = b.getElementsByTagName(a[1]); if (a[1] === "*") { var d = []; for (var e = 0; c[e]; e++) c[e].nodeType === 1 && d.push(c[e]); c = d } return c }), a.innerHTML = "<a href='#'></a>", a.firstChild && typeof a.firstChild.getAttribute !== "undefined" && a.firstChild.getAttribute("href") !== "#" && (l.attrHandle.href = function (a) { return a.getAttribute("href", 2) }), a = null } (), c.querySelectorAll && function () { var a = k, b = c.createElement("div"), d = "__sizzle__"; b.innerHTML = "<p class='TEST'></p>"; if (!b.querySelectorAll || b.querySelectorAll(".TEST").length !== 0) { k = function (b, e, f, g) { e = e || c; if (!g && !k.isXML(e)) { var h = /^(\w+$)|^\.([\w\-]+$)|^#([\w\-]+$)/.exec(b); if (h && (e.nodeType === 1 || e.nodeType === 9)) { if (h[1]) return p(e.getElementsByTagName(b), f); if (h[2] && l.find.CLASS && e.getElementsByClassName) return p(e.getElementsByClassName(h[2]), f) } if (e.nodeType === 9) { if (b === "body" && e.body) return p([e.body], f); if (h && h[3]) { var i = e.getElementById(h[3]); if (!i || !i.parentNode) return p([], f); if (i.id === h[3]) return p([i], f) } try { return p(e.querySelectorAll(b), f) } catch (j) { } } else if (e.nodeType === 1 && e.nodeName.toLowerCase() !== "object") { var m = e, n = e.getAttribute("id"), o = n || d, q = e.parentNode, r = /^\s*[+~]/.test(b); n ? o = o.replace(/'/g, "\\$&") : e.setAttribute("id", o), r && q && (e = e.parentNode); try { if (!r || q) return p(e.querySelectorAll("[id='" + o + "'] " + b), f) } catch (s) { } finally { n || m.removeAttribute("id") } } } return a(b, e, f, g) }; for (var e in a) k[e] = a[e]; b = null } } (), function () { var a = c.documentElement, b = a.matchesSelector || a.mozMatchesSelector || a.webkitMatchesSelector || a.msMatchesSelector, d = !1; try { b.call(c.documentElement, "[test!='']:sizzle") } catch (e) { d = !0 } b && (k.matchesSelector = function (a, c) { c = c.replace(/\=\s*([^'"\]]*)\s*\]/g, "='$1']"); if (!k.isXML(a)) try { if (d || !l.match.PSEUDO.test(c) && !/!=/.test(c)) return b.call(a, c) } catch (e) { } return k(c, null, null, [a]).length > 0 }) } (), function () { var a = c.createElement("div"); a.innerHTML = "<div class='test e'></div><div class='test'></div>"; if (a.getElementsByClassName && a.getElementsByClassName("e").length !== 0) { a.lastChild.className = "e"; if (a.getElementsByClassName("e").length === 1) return; l.order.splice(1, 0, "CLASS"), l.find.CLASS = function (a, b, c) { if (typeof b.getElementsByClassName !== "undefined" && !c) return b.getElementsByClassName(a[1]) }, a = null } } (), c.documentElement.contains ? k.contains = function (a, b) { return a !== b && (a.contains ? a.contains(b) : !0) } : c.documentElement.compareDocumentPosition ? k.contains = function (a, b) { return !!(a.compareDocumentPosition(b) & 16) } : k.contains = function () { return !1 }, k.isXML = function (a) { var b = (a ? a.ownerDocument || a : 0).documentElement; return b ? b.nodeName !== "HTML" : !1 }; var v = function (a, b) { var c, d = [], e = "", f = b.nodeType ? [b] : b; while (c = l.match.PSEUDO.exec(a)) e += c[0], a = a.replace(l.match.PSEUDO, ""); a = l.relative[a] ? a + "*" : a; for (var g = 0, h = f.length; g < h; g++) k(a, f[g], d); return k.filter(e, d) }; d.find = k, d.expr = k.selectors, d.expr[":"] = d.expr.filters, d.unique = k.uniqueSort, d.text = k.getText, d.isXMLDoc = k.isXML, d.contains = k.contains } (); var G = /Until$/, H = /^(?:parents|prevUntil|prevAll)/, I = /,/, J = /^.[^:#\[\.,]*$/, K = Array.prototype.slice, L = d.expr.match.POS, M = { children: !0, contents: !0, next: !0, prev: !0 }; d.fn.extend({ find: function (a) { var b = this.pushStack("", "find", a), c = 0; for (var e = 0, f = this.length; e < f; e++) { c = b.length, d.find(a, this[e], b); if (e > 0) for (var g = c; g < b.length; g++) for (var h = 0; h < c; h++) if (b[h] === b[g]) { b.splice(g--, 1); break } } return b }, has: function (a) { var b = d(a); return this.filter(function () { for (var a = 0, c = b.length; a < c; a++) if (d.contains(this, b[a])) return !0 }) }, not: function (a) { return this.pushStack(O(this, a, !1), "not", a) }, filter: function (a) { return this.pushStack(O(this, a, !0), "filter", a) }, is: function (a) { return !!a && d.filter(a, this).length > 0 }, closest: function (a, b) { var c = [], e, f, g = this[0]; if (d.isArray(a)) { var h, i, j = {}, k = 1; if (g && a.length) { for (e = 0, f = a.length; e < f; e++) i = a[e], j[i] || (j[i] = d.expr.match.POS.test(i) ? d(i, b || this.context) : i); while (g && g.ownerDocument && g !== b) { for (i in j) h = j[i], (h.jquery ? h.index(g) > -1 : d(g).is(h)) && c.push({ selector: i, elem: g, level: k }); g = g.parentNode, k++ } } return c } var l = L.test(a) ? d(a, b || this.context) : null; for (e = 0, f = this.length; e < f; e++) { g = this[e]; while (g) { if (l ? l.index(g) > -1 : d.find.matchesSelector(g, a)) { c.push(g); break } g = g.parentNode; if (!g || !g.ownerDocument || g === b) break } } c = c.length > 1 ? d.unique(c) : c; return this.pushStack(c, "closest", a) }, index: function (a) { if (!a || typeof a === "string") return d.inArray(this[0], a ? d(a) : this.parent().children()); return d.inArray(a.jquery ? a[0] : a, this) }, add: function (a, b) { var c = typeof a === "string" ? d(a, b) : d.makeArray(a), e = d.merge(this.get(), c); return this.pushStack(N(c[0]) || N(e[0]) ? e : d.unique(e)) }, andSelf: function () { return this.add(this.prevObject) } }), d.each({ parent: function (a) { var b = a.parentNode; return b && b.nodeType !== 11 ? b : null }, parents: function (a) { return d.dir(a, "parentNode") }, parentsUntil: function (a, b, c) { return d.dir(a, "parentNode", c) }, next: function (a) { return d.nth(a, 2, "nextSibling") }, prev: function (a) { return d.nth(a, 2, "previousSibling") }, nextAll: function (a) { return d.dir(a, "nextSibling") }, prevAll: function (a) { return d.dir(a, "previousSibling") }, nextUntil: function (a, b, c) { return d.dir(a, "nextSibling", c) }, prevUntil: function (a, b, c) { return d.dir(a, "previousSibling", c) }, siblings: function (a) { return d.sibling(a.parentNode.firstChild, a) }, children: function (a) { return d.sibling(a.firstChild) }, contents: function (a) { return d.nodeName(a, "iframe") ? a.contentDocument || a.contentWindow.document : d.makeArray(a.childNodes) } }, function (a, b) { d.fn[a] = function (c, e) { var f = d.map(this, b, c), g = K.call(arguments); G.test(a) || (e = c), e && typeof e === "string" && (f = d.filter(e, f)), f = this.length > 1 && !M[a] ? d.unique(f) : f, (this.length > 1 || I.test(e)) && H.test(a) && (f = f.reverse()); return this.pushStack(f, a, g.join(",")) } }), d.extend({ filter: function (a, b, c) { c && (a = ":not(" + a + ")"); return b.length === 1 ? d.find.matchesSelector(b[0], a) ? 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<!-- =======================================
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THE ACTUAL ORBIT SLIDER CONTENT
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======================================= -->
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<a href=""><img src="https://static.igem.org/mediawiki/2013/d/d9/Overflow.jpg" /></a>
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<!--Abstract Part-->
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<html>
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<div id="box1" align="justify">
<div id="box1" align="justify">
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</a>
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<h2><center>Special Thanks to Our 2013 iGEM Team Sponsors!</h2></center>
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<h2>ABSTRACT</h2>
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<center><img style="margin-bottom:0px; width: 500px; padding:2;"src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Logos/2012%20Sponsors.jpg" usemap="#sponsor">
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<p>
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<map name="sponsor">
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    The human race is growing fast. The world population is projected to top 10 billion by 2050. One response to the food supply needs of an increasing world population is creating better crop yield through crop survival. Pathogens and pests can cause catastrophic crop loss. Currently there is no widely used synthetic biological mechanism to help plants fight off bacterial pathogens and pests, so the main way large-scale growers protect crops is by using pesticides. We have engineered two possible solutions to address this problem.
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              <area shape="rect" coords="0,0,200,100" href="http://www.lifetechnologies.com/us/en/home.html" />
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              <area shape="rect" coords="200,0,450,100" href="http://systemsbiology.ucsf.edu/" />
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    In our first project, we have incorporated catalytically dead CAS9 (dCAS9) in conjunction with synthetic gRNAs complementary to pathogenic bacterial genes into a plasmid that naturally conjugates into neighboring bacterial cells. The specificity of the gRNAs will potentially allow us to target specific populations of bacterial pathogens in soil while leaving positive bacterial populations unharmed.
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    Our second project will provide plants with a sugar-mediated synthetic circuit that boosts a plant’s natural immune system and pesticide-producing capabilities in response to different levels of sugar a plant naturally produces in response to pests. The repression capabilities of dCAS9 will be used to regulate the synthetic circuit promoters that sense high or low sugar levels, reducing metabolic strain.
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<br>
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    </p>
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    <p>
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    </p>
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</div>
</div>
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<h2>Special Thanks to our 2013 iGEM Team Sponsors!</h2>
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<br><p>
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</html>
</html>

Latest revision as of 19:15, 27 September 2013

UCSF Home

UCSF iGEM 2013 Group Photo
Brainstorming project ideas during our second week of Bootcamp
Presenting a poster at the UCSF Center for Systems and Synthetic Biology retreat
Having a skype meeting with one of our mentors!
Human Practices: Speaking about Synthetic Biology at the SF Exploratorium
Having fun outside of the lab making ice cream with dry ice

Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome

In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. The goal of our project is to take advantage of a natural horizontal gene transfer mechanism in bacteria to precisely affect gene expression in selected strains. We combine bacterial conjugation with CRISPRi, an RNAi-like repression system developed from bacteria, to regulate gene expression in targeted strains within a complex microbial community. One possible application is to selectively repress pathogenic genes in a microbiome, leaving the community makeup unaffected. In addition, we use CRISPRi to lay the groundwork for transferring large circuits that enable complex functionality and decision-making in cells.

Team

Meet our dedicated team of under-graduates who conceived of, planned, and carried out the whole project.

Project

Discover our precision guided tools to target unique species in a complex microbiome and our groundwork for transferring large circuits that enable complex functionality and decision-making in cells.

Modeling

Take a look at how we use mathematical modeling to facilitate the design and construct of our project.

Human Practice

Examine how we teach Synthetic Biology to the general public through night event at Exploratorium and to high school students through a collaboration with Lincoln High School.

Special Thanks to Our 2013 iGEM Team Sponsors!


Retrieved from "http://2013.igem.org/Team:UCSF/lily2"