Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

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==Aug 16==
==Aug 16==
 +
===Colony PCR===
 +
<div class="experiment">
 +
<span class="author">Tatsui and Ashida</span>
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|8/15 BBa_I13504(RBS-GFP-DT)(1)||1174
 +
|-
 +
|8/15 BBa_I13504(RBS-GFP-DT)(2)||1174
 +
|-
 +
|8/15 BBa_I0462(RBS-luxR-DT)(1)||1174
 +
|-
 +
|8/15 BBa_I0462(RBS-luxR-DT)(2)||1174
 +
|-
 +
|8/15 BBa_K748002(lysis3)||1053
 +
|-
 +
|NC||--
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||1min||30cycles
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Sample||base pair
 +
|-
 +
|8/15 BBa_R0061(Plux)||344
 +
|-
 +
|8/15 BBa_K117000(lysis1)(1)||456
 +
|-
 +
|8/15 BBa_K117000(lysis1)(2)||456
 +
|-
 +
|8/15 BBa_B0015(DT)||443
 +
|-
 +
|8/15 BBa_I732020(RBS-lacZ&alpha;-DT)||703
 +
|-
 +
|NC||--
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
 +
|5min||30s||30s||40s||30cycles
 +
|}
 +
 +
===Electrophoresis===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||BBa_I13504(RBS-GFP-DT) -(1)
 +
|-
 +
|3||BBa_I13504(RBS-GFP-DT) -(2)
 +
|-
 +
|4||BBa_I0462(RBS-luxR-DT) -(1)
 +
|-
 +
|5||BBa_I0462(RBS-luxR-DT) -(2)
 +
|-
 +
|6||BBa_K748002(lysis3)
 +
|-
 +
|7||NC
 +
|-
 +
|8||100bp ladder
 +
|}
 +
[[File:igku_8161.jpg]]<br>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||BBa_R0061(Plux)
 +
|-
 +
|3||BBa_K117000(lysis1) -(1)
 +
|-
 +
|4||BBa_K117000(lysis1) -(1)
 +
|-
 +
|5||BBa_B0015(DT)
 +
|-
 +
|6||BBa_I732020(RBS-lacZ&alpha;-DT)
 +
|-
 +
|7||NC
 +
|-
 +
|8||100bp ladder
 +
|}
 +
[[File:igku_8162.jpg]]<br>
 +
</div>
 +
 +
===Master Plate===
 +
<div class="experiment">
 +
<span class="author">Tatsui</span>
 +
{| class="wikitable"
 +
!Number||Use LB plate (+CP)
 +
|-
 +
|1||BBa_I13504(RBS-GFP-DT)(1)
 +
|-
 +
|2||BBa_I13504(RBS-GFP-DT)(2)
 +
|-
 +
|3||BBa_K748002(lysis3)
 +
|-
 +
|4||BBa_R0061(Plux)
 +
|-
 +
|5||BBa_K117000(lysis1)(1)
 +
|-
 +
|6||BBa_K117000(lysis1)(2)
 +
|-
 +
|7||BBa_B0015(DT)
 +
|-
 +
|8||BBa_I732020(RBS-lacZ&alpha;-DT)
 +
|}
 +
{| class="wikitable"
 +
!Number||Use LB plate (+Amp)
 +
|-
 +
|1||BBa_I0462(RBS-luxR-DT)(1)
 +
|-
 +
|2||BBa_I0462(RBS-luxR-DT)(2)
 +
|}
 +
</div>
 +
 +
===Transformation===
 +
<div class="experiment">
 +
<span class="author">Hirano</span>
 +
{| class="wikitable"
 +
!Name||Sample||Competent Cells||Total||Plate
 +
|-
 +
|pBR322||1&micro;L||100&micro;L(XL-10gold)||101&micro;L||Amp
 +
|-
 +
|pBR322||1&micro;L||50&micro;L(takara)||51&micro;L||Amp
 +
|-
 +
|Pbad/araC||2&micro;L|||20&micro;L||22&micro;L||Kan
 +
|-
 +
|RBS-luxI-DT||2&micro;L|||20&micro;L||22&micro;L||Kan
 +
|}
 +
</div>
 +
 +
===Liquid Culture===
 +
<div class="experiment">
 +
<span class="author">Yoshdia</span>
 +
{| class="wikitable"
 +
!Sample||medium
 +
|-
 +
|8/15 BBa_I13504(RBS-GFP-DT))(1)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_I13504(RBS-GFP-DT)(2)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_I0462(RBS-luxR-DT)(2)||Plusgrow medium (+Amp)
 +
|-
 +
|8/15 BBa_K748002(lysis3)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_R0061(Plux)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_K117000(lysis1)(1)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_K117000(lysis1)(2)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_B0015(DT)||Plusgrow medium (+CP)
 +
|-
 +
|8/15 BBa_I732020(RBS-lacZ&alpha;-DT)||Plusgrow medium (+CP)
 +
|-
 +
|}
 +
</div>

Latest revision as of 19:31, 27 September 2013

Contents

Aug 16

Colony PCR

Tatsui and Ashida

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT)(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
NC--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plux)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-lacZα-DT)703
NC--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Electrophoresis

No name

LaneSample
1100bp ladder
2BBa_I13504(RBS-GFP-DT) -(1)
3BBa_I13504(RBS-GFP-DT) -(2)
4BBa_I0462(RBS-luxR-DT) -(1)
5BBa_I0462(RBS-luxR-DT) -(2)
6BBa_K748002(lysis3)
7NC
8100bp ladder

Igku 8161.jpg

LaneSample
1100bp ladder
2BBa_R0061(Plux)
3BBa_K117000(lysis1) -(1)
4BBa_K117000(lysis1) -(1)
5BBa_B0015(DT)
6BBa_I732020(RBS-lacZα-DT)
7NC
8100bp ladder

Igku 8162.jpg

Master Plate

Tatsui

NumberUse LB plate (+CP)
1BBa_I13504(RBS-GFP-DT)(1)
2BBa_I13504(RBS-GFP-DT)(2)
3BBa_K748002(lysis3)
4BBa_R0061(Plux)
5BBa_K117000(lysis1)(1)
6BBa_K117000(lysis1)(2)
7BBa_B0015(DT)
8BBa_I732020(RBS-lacZα-DT)
NumberUse LB plate (+Amp)
1BBa_I0462(RBS-luxR-DT)(1)
2BBa_I0462(RBS-luxR-DT)(2)

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
pBR3221µL100µL(XL-10gold)101µLAmp
pBR3221µL50µL(takara)51µLAmp
Pbad/araC2µL20µL22µLKan
RBS-luxI-DT2µL20µL22µLKan

Liquid Culture

Yoshdia

Samplemedium
8/15 BBa_I13504(RBS-GFP-DT))(1)Plusgrow medium (+CP)
8/15 BBa_I13504(RBS-GFP-DT)(2)Plusgrow medium (+CP)
8/15 BBa_I0462(RBS-luxR-DT)(2)Plusgrow medium (+Amp)
8/15 BBa_K748002(lysis3)Plusgrow medium (+CP)
8/15 BBa_R0061(Plux)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(1)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(2)Plusgrow medium (+CP)
8/15 BBa_B0015(DT)Plusgrow medium (+CP)
8/15 BBa_I732020(RBS-lacZα-DT)Plusgrow medium (+CP)
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