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Team:BYU Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog
From 2013.igem.org
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| - | + | : '''Large Phage July - August Notebook: September 16 - September 27 Daily Log'''</font> | |
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| + | : <u> '''Large Phage''' </u> </font> | ||
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| + | : [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]] | ||
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| + | : [[Team:BYU Provo/Notebook/LargePhage/Springexp|May-June]] | ||
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| + | : [[Team:BYU Provo/Notebook/LargePhage/Summerexp|July-August]] | ||
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| + | : [[Team:BYU Provo/Notebook/LargePhage/Fallexp|September-October]] | ||
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| + | <font size="4"> '''9/16/13''' </font> | ||
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| + | Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight. | ||
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| + | <font size="4"> '''9/18/13''' </font> | ||
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| + | We looked at the results from the spot test today. We found that: | ||
| + | All fractions had phage at 10^0 | ||
| + | Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. | ||
| + | Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4. | ||
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| + | We also measured the capsids and tails of the TEM images. | ||
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| + | [[File:September18-1.jpg|400px]] | ||
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| + | <font size="4"> '''9/23/13''' </font> | ||
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| + | We spent our time in class and over the weekend measuring the phage capsids and tails to see if there is a correlation between their sizes before and after our mutagenesis. | ||
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| + | <font size="4"> '''9/25/13''' </font> | ||
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| + | -Today we worked on the project poster image for the phage diagram | ||
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Latest revision as of 20:00, 27 September 2013
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9/16/13 Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.
9/18/13 We looked at the results from the spot test today. We found that: All fractions had phage at 10^0 Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4. We also measured the capsids and tails of the TEM images.
9/23/13 We spent our time in class and over the weekend measuring the phage capsids and tails to see if there is a correlation between their sizes before and after our mutagenesis.
9/25/13 -Today we worked on the project poster image for the phage diagram
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