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Team:UT Dallas/project part2
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<span class="title_spans">Dextranase and Norspermidine</span> <br><br> | <span class="title_spans">Dextranase and Norspermidine</span> <br><br> | ||
<b>How It works</b><br> | <b>How It works</b><br> | ||
| - | Another way to detect S. mutans in the mouth is to detect dextran through dextranase. Dextranase is an enzyme | + | Another way to detect S. mutans in the mouth is to detect dextran through dextranase. Dextranase is an enzyme and surface protein that contains a binding domain for Dextran, a very complex, branched polymer of glucans. When Dextranase is on the surface of bacteria, it binds to Dextran and holds the polymer to the bacteria. We presently believe that Dextranase constitutively works as a surface protein in E. Coli. <br><br> |
<b>What We Did</b><br> | <b>What We Did</b><br> | ||
| - | By using dextranase, we may be able to keep E. Coli in close proximity to S. Mutans, thereby effectively delivering destructive factors to S. Mutans. The dex gene would be included in the E. Coli plasmid to transcribe as a surface protein.<br><br> | + | By using dextranase, we may be able to keep E. Coli in close proximity to S. Mutans, thereby effectively delivering destructive factors to S. Mutans. The dex gene would be included in the E. Coli plasmid to transcribe as a surface protein.<br><br> |
<font size=5>NspC/Norspermidine</font><br><br> | <font size=5>NspC/Norspermidine</font><br><br> | ||
<b>How It Works</b><br> | <b>How It Works</b><br> | ||
| - | Norspermidine is a compound that breaks down biofilm by directly interacting with the | + | Norspermidine is a compound that breaks down biofilm by directly interacting with the exopolysaccharides of S. mutans. Norspermidine does this by interacting with charged or polar groups in secondary structure of the exopolysaccharide. <br><br> |
<b>What We Did</b><br> | <b>What We Did</b><br> | ||
| - | + | By determining the presence of S. Mutans in the mouth, we can deliver molecules to stop bacteria growth. We have now determined two ways to accomplish this: <br> | |
| - | By destroying the biofilm | + | By destroying the biofilm in which the S. Mutans grows through the action of NspC<br> |
By tightly controlling the populations of S. Mutans through CSP and effectively killing all bacteria. <br> | By tightly controlling the populations of S. Mutans through CSP and effectively killing all bacteria. <br> | ||
The gene NspC codes for Norspermidine and could be added to our detection systems.<br> | The gene NspC codes for Norspermidine and could be added to our detection systems.<br> | ||
<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/dexnspc_zpsc539e9fb.png" border="0" alt=" photo dexnspc_zpsc539e9fb.png"/ width=500> | <img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/dexnspc_zpsc539e9fb.png" border="0" alt=" photo dexnspc_zpsc539e9fb.png"/ width=500> | ||
Revision as of 20:31, 27 September 2013
Dextranase and Norspermidine
How It works
Another way to detect S. mutans in the mouth is to detect dextran through dextranase. Dextranase is an enzyme and surface protein that contains a binding domain for Dextran, a very complex, branched polymer of glucans. When Dextranase is on the surface of bacteria, it binds to Dextran and holds the polymer to the bacteria. We presently believe that Dextranase constitutively works as a surface protein in E. Coli.
What We Did
By using dextranase, we may be able to keep E. Coli in close proximity to S. Mutans, thereby effectively delivering destructive factors to S. Mutans. The dex gene would be included in the E. Coli plasmid to transcribe as a surface protein.
NspC/Norspermidine
How It Works
Norspermidine is a compound that breaks down biofilm by directly interacting with the exopolysaccharides of S. mutans. Norspermidine does this by interacting with charged or polar groups in secondary structure of the exopolysaccharide.
What We Did
By determining the presence of S. Mutans in the mouth, we can deliver molecules to stop bacteria growth. We have now determined two ways to accomplish this:
By destroying the biofilm in which the S. Mutans grows through the action of NspC
By tightly controlling the populations of S. Mutans through CSP and effectively killing all bacteria.
The gene NspC codes for Norspermidine and could be added to our detection systems.
<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/dexnspc_zpsc539e9fb.png" border="0" alt=" photo dexnspc_zpsc539e9fb.png"/ width=500>
