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Team:Shenzhen BGIC ATCG/parts
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Revision as of 20:32, 27 September 2013
Playing with my eyes
aren't you?
Hi I am Dr. Mage!
A "budding" yeast cell!
Best BioBricks
Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306
K1051303
K1051300
K1051301
K1051302
K1051304
K1051305
K1051306
Best BioBrick (Engineered), BBa_K1051900
K1051900a
K1051900b
Best Measurement, BBa_K1051257 ~ BBa_K1051259
K1051257
K1051258
K1051259
Devices List
Final Constructions
Final Constructions of E.coli
K1051410
K1051412
K1051414
Final Constructions of Budding Yeast
K1051900a
K1051900b
K1051901
K1051902
K1051903
Promoter Verification
Verification of E.coli
K1051245
K1051246
K1051247
Verification of Budding Yeast
K1051307
K1051355
K1051356
K1051357
K1051358
K1051359
K1051365
K1051366
K1051367
K1051368
K1051369
Degradation Rate Measurement
Measurement of E.coli
K1051257
K1051258
K1051259
Measurement of Budding Yeast
K1051278
K1051279
K1051280
K1051281
K1051288
Targeting Peptides Verification
K1051150
K1051151
K1051152
K1051153
CRISPRi Verification
K1051852
Alternative Splicing Verification
K1051706
Basic Parts
Universal Parts
Reporters
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051000 | Reporter | Stop codon free RFP in RFC[23] standard | Xiang LI | 675 |
| BBa_K1051001 | Reporter | Stop codon free ECFP in RFC[23] standard | Xiang LI | 716 |
| BBa_K1051002 | Reporter | Stop codon free EYFP in RFC[23] standard | Xiang LI | 717 |
| BBa_K1051003 | Reporter | Stop codon free GFP in RFC[23] standard | Xiang LI | 714 |
| BBa_K1051004 | Reporter | Stop codon free mOrange in RFC[23] standard | Xiang LI | 738 |
Yeast Terminators
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051006 | Terminator | Saccharomycescerevisiae TBY-1 terminator with stopcodon | Xiang LI | 31 |
| BBa_K1051007 | Terminator | S. cerevisiae SSV7 terminator with stopcodon | Xiang LI | 39 |
| BBa_K1051008 | Terminator | S. cerevisiae CAA terminator with stopcodon | Xiang LI | 18 |
E. coli Terminators
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051009 | Terminator | e.coli terminator ilvGEDA_T with stop codon | Xiang LI | 92 |
Cyclin Promoters
Yeast
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051300 | Regulatory | cln2 promoter (G1 phase to S phase) | Kequan Lin | 1000 |
| BBa_K1051301 | Regulatory | clb2 promoter (during G2 phase) | Kequan Lin | 1063 |
| BBa_K1051302 | Regulatory | clb5 promoter (during G1 phase) | Kequan Lin | 648 |
| BBa_K1051304 | Regulatory | cln3 promoter (M phase to G1 phase) | Kequan Lin | 1075 |
| BBa_K1051305 | Regulatory | met16 promoter (M phase to G1 phase) | Zhu Shuang | 551 |
| BBa_K1051306 | Regulatory | met28 promoter (S phase) | Zhu Shuang | 500 |
E. coli
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051400 | Regulatory | promoter->dnaAp1 | Bingwei Zheng | 359 |
| BBa_K1051401 | Regulatory | promoter->dnaAp1 no RBS | Bingwei Zheng | 184 |
| BBa_K1051402 | Regulatory | promoter->gyrB | Bingwei Zheng | 188 |
| BBa_K1051403 | Regulatory | promoter->gyrB no RBS | Bingwei Zheng | 158 |
| BBa_K1051404 | Regulatory | promoter->ftsQp | Bingwei Zheng | 485 |
| BBa_K1051405 | Regulatory | promoter->ftsQp no RBS | Bingwei Zheng | 198 |
Degradation Tags
Yeast
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051200 | DNA | ubiquitin | wanling ZHOU | 1146 |
| BBa_K1051201 | DNA | D-BOX | wanling ZHOU | 39 |
| BBa_K1051202 | DNA | cln1-PEST | wanling ZHOU | 489 |
| BBa_K1051203 | DNA | Cln2-PEST | wanling ZHOU | 537 |
| BBa_K1051204 | DNA | cln3-PEST | wanling ZHOU | 588 |
E. coli
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051206 | Tag | The degradation tag in E. coil ,M0050 with TAATAA. | Rui Guan | 39 |
| BBa_K1051207 | Tag | The degradation tag in E. coil ,M0051 with TAATAA. | Rui Guan | 45 |
| BBa_K1051208 | Tag | The degradation tag in E. coil ,M0052 with TAATAA. | Rui Guan | 39 |
Targeting Peptides
Mitochondria
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051100 | Coding | targeting peptide to mitochondria inner membrane | Jinchun Zhang | 209 |
| BBa_K1051101 | Coding | Cox4 targeting peptide to mitochondria inner membrane | Jinchun Zhang | 75 |
| BBa_K1051102 | Coding | targeting peptide to mitochondria inner membrane | Jinchun Zhang | 141 |
| BBa_K1051103 | Coding | TOM22, targeting peptide to mitochondria outer membrane | Jinchun Zhang | 69 |
| BBa_K1051104 | Coding | VDAC1;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 93 |
| BBa_K1051105 | Coding | TOM40;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 147 |
| BBa_K1051106 | Coding | targeting peptide to mitochondria outer membrane | Jinchun Zhang | 96 |
| BBa_K1051107 | Coding | Tom70;targeting peptide to mitochondria outer membrane | Jinchun Zhang | 90 |
| BBa_K1051108 | Coding | targeting peptide to mitochondria outer membrane;Tom20 | Jinchun Zhang | 120 |
| BBa_K1051109 | Coding | targeting peptide to mitochondria matrix;MIA40 | Jinchun Zhang | 93 |
Centrosome
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051110 | Coding | ScSfi1;targeting peptide to centrosome | Jinchun Zhang | 63 |
| BBa_K1051111 | Coding | ScSfi1E; targeting peptide to centrosome; | Jinchun Zhang | 63 |
| BBa_K1051112 | Coding | ScKar1;targeting peptide to centrosome | Jinchun Zhang | 60 |
| BBa_K1051113 | Coding | ScSac3;targeting peptide to centrosome | Jinchun Zhang | 57 |
Nucleus
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051114 | Coding | H2A2;targeting peptide to nucleus | Jinchun Zhang | 408 |
Vacuolar
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051115 | Coding | ZRC1;Vacuolar Membrane Targeting Protein | Jinchun Zhang | 396 |
Actin
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051116 | Coding | Actin Targeting Protein | Jinchun Zhang | 1779 |
Plasma Membrane
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051117 | Coding | Plasma Membrane Targeting Sequence | Jinchun Zhang | 12 |
Peroxisomes
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051118 | Coding | ScPEX21p；baking yeast targeting peptide to peroxisome ; | Jinchun Zhang | 30 |
CRISPRi Verification
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051800 | Coding | protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon. | Shihong Chen | 4164 |
| BBa_K1051801 | Device | Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib | Shihong Chen | 388 |
| BBa_K1051801 | Device | Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib | Shihong Chen | 388 |
Alternative Splicing Verification
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1051700 | DNA | SRC1 Intron -4bp at 5' | Yang Zhou | 129 |
| BBa_K1051701 | DNA | SRC1 Intron+GG | Yang Zhou | 132 |
| BBa_K1051702 | DNA | SRC1 Intron+CGG | Yang Zhou | 133 |
| BBa_K1051703 | DNA | SRC1 Intron with additional 6bp at both directions | Yang Zhou | 142 |
Parts Sequencing
All of our parts have been sequenced to get verification.
Sequencing Example of K1051006
