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Team:Washington/BlinkingTest
From 2013.igem.org
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1. Prepare 20 ml of <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a> in a 50 ml falcon tube.</br> | 1. Prepare 20 ml of <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a> in a 50 ml falcon tube.</br> | ||
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.</br> | 2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.</br> | ||
| - | 3. | + | 3. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the blinking timer as follows:</a></br> |
- always green light.</br> | - always green light.</br> | ||
- 600 millisecond green wait and 400 millisecond black wait.</br> | - 600 millisecond green wait and 400 millisecond black wait.</br> | ||
Revision as of 21:01, 27 September 2013
M9 + Casamino Acid Plate 1. Prepare 20 ml of M9 + Casamino Acid Media in a 50 ml falcon tube. 2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest. 3. Set the blinking timer as follows: - always green light. - 600 millisecond green wait and 400 millisecond black wait. - 125 millisecond green wait and 875 millisecond black wait. - 25 millisecond green wait and 975 millisecond black wait. - 5 millisecond green wait and 995 millisecond black wait. - always dark. 4. Follow steps of the 96-Well Plate Assay.
