Team:Washington/BlinkingTest

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1. Prepare 20 ml of <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a> in a 50 ml falcon tube.</br>
1. Prepare 20 ml of <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a> in a 50 ml falcon tube.</br>
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.</br>
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.</br>
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3. The blinking timer were set up as follows: </br>
+
3. <a href = "https://2013.igem.org/Team:Washington/appsetup#Blinking">Set the blinking timer as follows:</a></br>
- always green light.</br>
- always green light.</br>
- 600 millisecond green wait and 400 millisecond black wait.</br>
- 600 millisecond green wait and 400 millisecond black wait.</br>

Latest revision as of 21:09, 27 September 2013


M9 + Casamino Acid Plate
1. Prepare 20 ml of M9 + Casamino Acid Media in a 50 ml falcon tube.
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.
3. Set the blinking timer as follows:
- always green light.
- 600 millisecond green wait and 400 millisecond black wait.
- 125 millisecond green wait and 875 millisecond black wait.
- 25 millisecond green wait and 975 millisecond black wait.
- 5 millisecond green wait and 995 millisecond black wait.
- always dark.
4. Follow steps of the 96-Well Plate Assay.