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Team:Washington/Protocols
From 2013.igem.org
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<b>Light Sensing Media:</b> | <b>Light Sensing Media:</b> | ||
| - | <li><a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></li> | + | <ul><li><a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></li> |
<li><a href = "https://2013.igem.org/Team:Washington/M9Media">M9 Media + Casamino Acid Recipe</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/M9Media">M9 Media + Casamino Acid Recipe</a></li> | ||
| - | <li><a href = "https://2013.igem.org/Team:Washington/M9Plate">M9 + Casamino Acid Plate</a></li> | + | <li><a href = "https://2013.igem.org/Team:Washington/M9Plate">M9 + Casamino Acid Plate</a></li></ul> |
| - | <b>Light Testing:</b> | + | <b>Light Testing:</b><ul> |
<li><a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">General Protocol</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">General Protocol</a></li> | ||
<li><a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a></li> | ||
| Line 48: | Line 48: | ||
<li><a href = "https://2013.igem.org/Team:Washington/GFPExpression">GFP Expression on Agar Plates</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/GFPExpression">GFP Expression on Agar Plates</a></li> | ||
<li><a href = "https://2013.igem.org/Team:Washington/LightIntensityTest">Light Intensity Test</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/LightIntensityTest">Light Intensity Test</a></li> | ||
| - | <li><a href = "https://2013.igem.org/Team:Washington/BlinkingTest">Blinking Test</a></li> | + | <li><a href = "https://2013.igem.org/Team:Washington/BlinkingTest">Blinking Test</a></li></ul> |
| - | <b>Setting Up The App:</b> | + | <b>Setting Up The App:</b><ul> |
| - | <li><a href = "https://2013.igem.org/Team:Washington/appsetup">E.colight Setup</a></li> | + | <li><a href = "https://2013.igem.org/Team:Washington/appsetup">E.colight Setup</a></li></ul> |
| - | <b>App Testing:</b> | + | <b>App Testing:</b><ul> |
<li><a href = "https://2013.igem.org/Team:Washington/BleedThroughTest">Bleed-Through Test</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/BleedThroughTest">Bleed-Through Test</a></li> | ||
| + | </ul> | ||
</body> | </body> | ||
<br> | <br> | ||
</html> | </html> | ||
Revision as of 21:35, 27 September 2013
Cloning Protocols Workflow:
- 1) Isolation of Plasmid DNA (miniprep)
- 2) General PCR Protocol (also see PCR GoTaq - product (30-200ng/ ul) Check on gel PCR Purification and/or Dpnl Digest
- 3) General Digestion Protocol Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)
- 4) Agarose Gel Electrophoresis
- 5) General Ligation Protocol (Don’t forget background control plates) Heat Inactivation (optional - up to 10 fold increase) - 65° for 10 minutes
- 6) Heat shock/chemical competent transformation
- 7) Colony PCR with Green taq Miniprep (stocks can be made from this culture - add 1ml extra)
- 9) Making Glycerol Frozen Stocks
Light Sensing Protocols
Light Sensing Media:
Light Testing:Setting Up The App: App Testing:
- General Protocol
- 96-Well Plate Assay
- 60 mm Petri Dish Assay
- Fluorescent Analysis Protocol
- GFP Expression on Agar Plates
- Light Intensity Test
- Blinking Test
