Team:SJTU-BioX-Shanghai

From 2013.igem.org

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Revision as of 22:26, 27 September 2013

Abstract

How to regulate an entire metabolic pathway in vivo, delicately, accurately and conveniently, simultaneously controlling the expression of several genes? And how to optimize metabolic fluxes so as to maximize desired products? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the genome, or have been implemented into the genome.

So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating CRISPRi, the newly developed expression interference tool, with three light sensors (namely red light sensor, green light sensor and blue light sensor), we expect to provide a versatile platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to change the target, simply change the small guide RNA (sgRNA)!

In addition, with the luminous device and accompanying software, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct flux balance analysis (FBA), giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into intensity of lights that are finally to be exerted on cell cultures. It is -- just convenient! :)

Luminous System provide us a fascinating tool to adjust expression on genomic level.

Independent luminous sources work through a unbelievable project

Achievements

The breakthrough we made: The quantitative regulation of gene expression in the level of genome.

The system we built: Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.

The device we created : An electronic device constructed to measure the parameters of LED.

Helping other teams: sending parts to Zhejiang University and the Chinese University of Hong Kong, helping improving the modeling of the projects of NTU-Taida and NTU Taiwan in the 2013 conference, help students from Shandong University to construct their own team.

The experiment we did: having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.

Parts we submitted: .

Human practise: involvement in an official academic elite cultivation plan supported by national education ministry. Cooperation with corporation to try applying our project into industry. A survey associated with subject cross and a detailed relevant analysis of this survey.

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 				<p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> The quantitative regulation of gene expression in the level of genome. </p>
 				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The system we built:</font></strong> Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.</p>
-				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created :</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p>
+				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created :</font></strong> An electronic device constructed to measure the parameters of LED.</p>
-				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> device we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched. </p>
+				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Helping other teams:</font></strong> sending parts to Zhejiang University and the Chinese University of Hong Kong, helping improving the modeling of the projects of NTU-Taida and NTU Taiwan in the 2013 conference, help students from Shandong University to construct their own team. </p>
 				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The experiment we did:</font></strong> having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.</p>
 				<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> .</p>