Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Explist

From 2013.igem.org

(Difference between revisions)
(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: June 10 - June 23 Experimen...")
 
(5 intermediate revisions not shown)
Line 10: Line 10:
|- valign="top"
|- valign="top"
-
| style="width: 18%; background-color: transparent;"|
+
| style="width: 20%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Small_Phage|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
Line 26: Line 28:
</font>
</font>
-
| style="width: 82%; background-color: transparent;"|
+
| style="width: 80%; background-color: transparent;"|
<font face="Calibri" size="3">
<font face="Calibri" size="3">
Line 38: Line 40:
<br>
<br>
-
<font size="4"> '''6.5 Bacteria Cultures''' </font>
+
<font size="4"> '''6.10 RNase Preparation''' </font>
-
This procedure grows bacteria for future use.
+
Prepare RNase A to be used in the purification procedure.
-
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
+
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.10RNasePreparation|6.10 RNase A Preparation]]
<br>
<br>
-
<font size="4"> '''6.7 Phage Suspension Buffer Preparation''' </font>
+
<font size="4"> '''6.12 PEG Purification''' </font>
-
This procedure creates 1 L of 10x phage suspension buffer.
+
Purify phage to be used in a CsCl gradient.
-
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.5 Phage Suspension Buffer Preparation]]
+
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.12PEGPurification|6.12 PEG Purification]]
<br>
<br>

Latest revision as of 00:12, 28 September 2013


Phage Purification May - June Notebook: June 10 - June 23 Experiment Listing



Phage Purification
March-April
May-June
July-August
September-October

6.10 Phage Preparation

This procedure prepares phage to be purified with the purification procedure.

Detailed procedure: 6.10 Phage Preparation


6.10 RNase Preparation

Prepare RNase A to be used in the purification procedure.

Detailed procedure: 6.10 RNase A Preparation


6.12 PEG Purification

Purify phage to be used in a CsCl gradient.

Detailed procedure: 6.12 PEG Purification