Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period8/Dailylog

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| colspan="3" | <font color="#333399" size="5" font face="Calibri">'''Phage Purification May - June Notebook: May 27 - June 9 Daily Log'''</font>
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| colspan="3" | <font color="#333399" size="5" font face="Calibri">'''Phage Purification July Notebook: July 1 - July 15 Daily Log'''</font>
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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''5/29/13''' </font>
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<font size="4"> '''7/1/13''' </font>
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- Finished the PEG purification.
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Reviewed results of the titer from last class.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.26_PEG_Purification|5.26 PEG Purification]]
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<font size="4"> '''5/31/13''' </font>
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<font size="4"> '''7/3/13''' </font>
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- Worked on setting up our notebook online.
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Prepared phage for an EM.
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[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.3 EM Phage Preparation |7.3 EM Phage Preparation]]
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<font size="4"> '''6/3/13''' </font>
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<font size="4"> '''7/5/13''' </font>
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- Ran the CsCl purification step.
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Prepared streaks of E. coli B. and BL21 using streaks from last Friday.
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[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.5 Bacterial Preparation|7.5 Bacterial Preparation]]
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]]
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<font size="4"> '''7/8/13''' </font>
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- Next class we will attempt extracting the phage from the gradient.
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Worked on the wiki.  Prepared for our outreach to the community activity.
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<font size="4"> '''6/5/13''' </font>
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<font size="4"> '''7/10/13''' </font>
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- Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle.   
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Took pictures of our purified phage using an EM.  We noticed large amounts of crystalline structure in the solution that we believe to be CsCl. We believe that increasing the dialysis time will help purify the CsCl better and remove these crystalline structures.  
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: Only extracted T4 because the T7 gradient was destroyed in the refrigerator.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.10 T7 Phage Pictures|7.10 T7 Phage Pictures]]
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: Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.
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: Placed these in the refrigerator.
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- Prepared stocks of bacteria for future use.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.5 Bacteria Cultures|6.5 Bacteria Cultures]]
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<font size="4"> '''6/7/13''' </font>
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<font size="4"> '''7/12/13''' </font>
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- Made 1 L of 10 X phage suspension buffer.
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: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation|6.7 Phage Suspension Buffer Preparation]]
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Prepared streaks of E. coli B. and BL21 using streaks from last Friday.
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[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.12 Bacterial Preparation|7.12 Bacterial Preparation]]
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Latest revision as of 00:18, 28 September 2013


Phage Purification July Notebook: July 1 - July 15 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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7/1/13

Reviewed results of the titer from last class.


7/3/13

Prepared phage for an EM. 7.3 EM Phage Preparation


7/5/13

Prepared streaks of E. coli B. and BL21 using streaks from last Friday. 7.5 Bacterial Preparation


7/8/13

Worked on the wiki. Prepared for our outreach to the community activity.


7/10/13

Took pictures of our purified phage using an EM. We noticed large amounts of crystalline structure in the solution that we believe to be CsCl. We believe that increasing the dialysis time will help purify the CsCl better and remove these crystalline structures.

7.10 T7 Phage Pictures


7/12/13

Prepared streaks of E. coli B. and BL21 using streaks from last Friday. 7.12 Bacterial Preparation

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