Team:UPenn/Notebook

From 2013.igem.org

(Difference between revisions)
m
 
(3 intermediate revisions not shown)
Line 2: Line 2:
<head>
<head>
<script src="https://googledrive.com/host/0B4ZBZOYYKBzETkFqdnhMeV9fMzA" ></script>
<script src="https://googledrive.com/host/0B4ZBZOYYKBzETkFqdnhMeV9fMzA" ></script>
 +
<link href="https://googledrive.com/host/0B4ZBZOYYKBzEYUtsRS1ZWHM2bTQ" type="text/css" rel="stylesheet"/>
     <style>
     <style>
-
 
-
.outer {
 
-
width: 100%;
 
-
text-align: center;
 
-
float: center;
 
-
height: 100%;
 
-
background-color: white;
 
-
}
 
-
 
-
#p-logo {
 
-
    position: absolute;
 
-
    display: none;
 
-
}
 
-
 
-
#content {
 
-
width: 100%;
 
-
background-color: white;
 
-
}
 
-
 
-
#top-section {
 
-
    position: absolute;
 
-
}
 
-
 
-
 
-
.firstHeading {
 
-
    position: absolute;
 
-
    display: none;
 
-
}
 
-
 
-
.thumb {
 
-
    display: none;
 
-
    position: absolute;
 
-
}
 
-
 
-
#menubar {
 
-
    display: none;
 
-
    position: absolute;
 
-
}
 
-
.noprint {
 
-
    display: none;
 
-
    position: absolute;
 
-
}
 
-
#footer {
 
-
    display: none;
 
-
    position: absolute;
 
-
}
 
-
 
-
 
-
/*navigation*/
 
-
.nav {
 
-
    padding: 0px;
 
-
    width: 100%;
 
-
    background-color: white;
 
-
    position: fixed;
 
-
    margin: auto;
 
-
    z-index: 999;
 
-
}
 
-
 
-
.nav li {
 
-
    display: inline;
 
-
    margin: 0px 30px 30px 0px;
 
-
    font-family: Verdana, sans-serif;
 
-
}
 
-
 
/*Last year's notbook style code*/
/*Last year's notbook style code*/
Line 208: Line 145:
     <ul class="nav">
     <ul class="nav">
         <li><a href="https://2013.igem.org/Team:UPenn">Home</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn">Home</a></li>
-
         <li>Team</li>
+
         <li><a hred="https://2013.igem.org/Team:UPenn#three">Team</a></li>
-
         <li>Official&nbsp;&nbsp;&nbsp;Team&nbsp;&nbsp;&nbsp;&nbsp; Profile</li>
+
         <li>Official Team Profile</li>
         <li><a href="https://2013.igem.org/Team:UPenn/Project">Project</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Project">Project</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Parts">Parts</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Parts">Parts</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Modeling">Modeling</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Modeling">Modeling</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Notebook">Notebook</a></li>
         <li><a href="https://2013.igem.org/Team:UPenn/Notebook">Notebook</a></li>
-
        <li>Safety</li>
+
          <li><a href="https://2013.igem.org/Team:UPenn/Safety">Safety</a></li>
          
          
     </ul>
     </ul>

Latest revision as of 15:18, 1 July 2013

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

 

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

 

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Retrieved from "http://2013.igem.org/Team:UPenn/Notebook"