Team:UPenn/Notebook

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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!align="center"|[[Team:UPenn|Home]]
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!align="center"|[[Team:UPenn/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=UPenn Official Team Profile]
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!align="center"|[[Team:UPenn/Project|Project]]
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!align="center"|[[Team:UPenn/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UPenn/Modeling|Modeling]]
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!align="center"|[[Team:UPenn/Notebook|Notebook]]
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!align="center"|[[Team:UPenn/Safety|Safety]]
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!align="center"|[[Team:UPenn/Attributions|Attributions]]
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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    <div class="outer">
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    <ul class="nav">
 +
        <li><a href="https://2013.igem.org/Team:UPenn">Home</a></li>
 +
        <li><a hred="https://2013.igem.org/Team:UPenn#three">Team</a></li>
 +
        <li>Official Team Profile</li>
 +
        <li><a href="https://2013.igem.org/Team:UPenn/Project">Project</a></li>
 +
        <li><a href="https://2013.igem.org/Team:UPenn/Parts">Parts</a></li>
 +
        <li><a href="https://2013.igem.org/Team:UPenn/Modeling">Modeling</a></li>
 +
        <li><a href="https://2013.igem.org/Team:UPenn/Notebook">Notebook</a></li>
 +
          <li><a href="https://2013.igem.org/Team:UPenn/Safety">Safety</a></li>
 +
       
 +
    </ul>
 +
   
 +
 
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<iframe src='http://embed.verite.co/timeline/?source=0AoZBZOYYKBzEdDA1dUFNaU93QzQ4LURURjJfdzRiVFE&font=Bevan-PotanoSans&maptype=toner&lang=en&height=650' width='100%' height='650' frameborder='0'></iframe>
 +
   
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<p style="text-align:center;color:white;">June 2012 Notebook</p>
 +
 
 +
<div id="accordion-container">
 +
<h2 class="accordion-header">Week 1</h2>
 +
 +
<div class="accordion-content">
 +
<p><b>June 6th</b></p>
 +
                                                <ul>
 +
                                                  <li>Set up some lab equipment</li>
 +
                                                  <li>Autoclaved for a while</li>
 +
                                                  <li>Organized biobrick stuff</li>
 +
                                                  <li>Called Vinoo about DNA planning</li>
 +
 
 +
                                                </ul> 
 +
<br>
 +
 
 +
<p><b>June 7th</b></p>
 +
                                                <ul>
 +
                                                  <li>Transformed Cph8, pLsr, and LuxS</li>
 +
                                                  <li>Placed order with Vinoo</li>
 +
<li>Developed idea using PGY/PCN system to activate a gene</li>
 +
                                                </ul>                   
 +
 +
 +
 +
</div>
 +
 +
<h2 class="accordion-header">Week 2</h2>
 +
 +
<div class="accordion-content">
 +
 +
<p><b>June 11th</b></p>
 +
                            &nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>PCR'd mCherry from NAS157</li>
 +
                                                  <li>Ran 1% Gel and purified product</li>
 +
 
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Designed primers for LsR promoter</li>
 +
<li>Meeting with Dr. Sarkar</li>
 +
</ul>
 +
<br>
 +
<p><b>June 12th</b></p>
 +
                            &nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>Digested mCherry PCR product with BamHI and NotI</li>
 +
                                                  <li>Column purified mCherry and ligated into NAS152 backbone</li>
 +
<li>Transformed NAS152-mCherry into DH5alpha</li>
 +
<li>Poured 25 LB-Kan plates</li>
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Research more information about bacterial drug delivery system</li>
 +
<li>More research into biofilm project</li>
 +
</ul>
 +
<br>
 +
<p><b>June 14th</b></p>
 +
 
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Met with Dr. Goulian, obtained pDawn and pDusk</li>
 +
<li>Identified inaK as a surface display gene we can use</li>
 +
</ul>
 +
</div>
 +
 +
<h2 class="accordion-header">Week 3</h2>
 +
 +
<div class="accordion-content">
 +
 +
<p>June 18th</p>
 +
 
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
<ul>
 +
<li>Miniprep pDawn and pDusk</li>
 +
<li>Test cut pDawn and pDusk with XmaI, analytical gel was correct</li>
 +
<li>Prep cut pDawn and pDusk with BamHI and NotI, gel purified</li>
 +
</ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Ordered and picked up PCR purification kit from cell center</li>
 +
<li>Additional orders through cell center</li>
 +
<li>Designed primers for one of Peter's components (forgot which)</li>
 +
</ul>
 +
<br>
 +
 
 +
<p>June 20</p>
 +
 
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
<ul>
 +
<li>Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan</li>
 +
<li>PCR purified fragments (Peter), then ran gel?</li>
 +
</ul>
 +
 
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Researched DARPin binding domains and linkers</li>
 +
<li>Finalized some biobrick orders</li>
 +
<li>Finalized synthesis order (minus linker)</li>
 +
</ul>
 +
 
 +
</div>
 +
 
 +
 
 +
</div>
 +
 
 +
</body>
 +
</html>

Latest revision as of 15:18, 1 July 2013

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

 

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

 

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Retrieved from "http://2013.igem.org/Team:UPenn/Notebook"