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| | <div class="text" style="top: 160px;"> | | <div class="text" style="top: 160px;"> |
| - | <h2>uOttawa iGEM 2013</h2>
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| | <h3>What is fold-change detection?</h3> | | <h3>What is fold-change detection?</h3> |
| | <p style="width: 800px;"> | | <p style="width: 800px;"> |
| - | Most detection systems will look for the absolute concentration of a certain molecule. This year, we designed and built a <b>fold-change detector</b>, which is responsive to relative changes in input. Using this method, we can make our detector resistant to background noise, expand dynamic range, and reduce dependence on expensive analysis methods such as flow cytometry or fluorescence microscopy. | + | Many synthetic gene networks are susceptible to cellular noise, as they rely upon the absolute levels of gene regulators which can vary greatly between individual cells. To address this, uOttawa has engineered a network in <i>S. cerevisiae</i> that is responsive to <b>fold-changes</b> as opposed to absolute changes in stimulus. This allows the network to maintain sensitivity despite noise, and also permits response to stimuli in a much larger dynamic range. By modifying the promoter driving the stimulus, the network can be engineered to detect fold-changes of any molecule with a responsive promoter, thereby serving as a <b>structural chassis for the next generation of molecule detectors</b>. |
| | <a href="/Team:uOttawa/project">Learn more.</a> | | <a href="/Team:uOttawa/project">Learn more.</a> |
| | </p> | | </p> |
| | </div> | | </div> |
| - | <div id="text1-2" style="top: 430px;"> | + | <div id="text1-2" style="top: 480px;"> |
| | Scroll down | | Scroll down |
| | <img src="https://static.igem.org/mediawiki/2013/1/19/Uo2013-arrow.png" alt="" /> | | <img src="https://static.igem.org/mediawiki/2013/1/19/Uo2013-arrow.png" alt="" /> |
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| | <h3>How can we improve the process of designing genes?</h3> | | <h3>How can we improve the process of designing genes?</h3> |
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| - | Efficient tools for synthetic biology are often expensive. This year, we developed a web-based application that intelligently queries the iGEM registry and allows you to construct genes from available parts. This application, called "bricklayer", will also automatically suggest assembly methods, construct primers, and calculate chemical properties of desired DNA.<br /> | + | Efficient tools for synthetic biology are often expensive. This year, we developed a web-based application that intelligently queries the iGEM registry and allows you to construct genes from available parts. This application, called <i>Bricklayer</i>, will also automatically suggest assembly methods, construct primers, and calculate chemical properties of desired DNA.<br /> |
| | <a href="/Team:uOttawa/software">Learn more.</a> | | <a href="/Team:uOttawa/software">Learn more.</a> |
| | </p> | | </p> |
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| | <div class="text text-center" style="top: 1350px;"> | | <div class="text text-center" style="top: 1350px;"> |
| - | <h3>Can we promote science and synthetic biology among schoolchildren?</h3> | + | <h3>Can we promote science and synthetic biology among school children?</h3> |
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| | Our Human Practices team wrote, illustrated, and published a children's book following the adventures of Mr. Cool, a hilariously klutzy scientist who, with the help of his yeast colonies and some clever genetic engineering, can solve all kinds of problems on the microscopic scale. | | Our Human Practices team wrote, illustrated, and published a children's book following the adventures of Mr. Cool, a hilariously klutzy scientist who, with the help of his yeast colonies and some clever genetic engineering, can solve all kinds of problems on the microscopic scale. |
| | <a href="/Team:uOttawa/humanpractices">Learn more.</a> | | <a href="/Team:uOttawa/humanpractices">Learn more.</a> |
| | </p> | | </p> |
| - | </div>
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| - | <div id="footer" class="text-right" style="top: 1550px;">
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| - | © uOttawa iGEM Team 2013. This is frigging awesoooooome.
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| | </div> | | </div> |
| | </div> | | </div> |
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| | <h3>How can we improve the process of designing genes?</h3> | | <h3>How can we improve the process of designing genes?</h3> |
| | <p> | | <p> |
| - | Efficient tools for synthetic biology are often expensive. This year, we developed a web-based application that intelligently queries the iGEM registry and allows you to construct genes from available parts. This application, called "bricklayer", will also automatically suggest assembly methods, construct primers, and calculate chemical properties of desired DNA. | + | Efficient tools for synthetic biology are often expensive. This year, we developed a web-based application that intelligently queries the iGEM registry and allows you to construct genes from available parts. This application, called <i>Bricklayer</i>, will also automatically suggest assembly methods, construct primers, and calculate chemical properties of desired DNA. |
| | <a href="/Team:uOttawa/software">Learn more.</a> | | <a href="/Team:uOttawa/software">Learn more.</a> |
| | </p> | | </p> |
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