Team:ITB Indonesia

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<img src="https://static.igem.org/mediawiki/igem.org/6/65/ITB_Indonesia-slide1.jpg" alt=""/>
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<div class="carousel-caption">
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<p><a href="https://2013.igem.org/Team:ITB_Indonesia/BehindTheScene/DangerAflatoxin">How Dangerous Aflatoxin for Our Bodies?</a></p>
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<img src="https://static.igem.org/mediawiki/igem.org/4/40/ITB_Indonesia-slide2.jpg" alt=""/>
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<div class="carousel-caption">
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<p><a href="https://2013.igem.org/Team:ITB_Indonesia/BehindTheScene/AflatoxinWholeCellBiosensor">Aflatoxin Whole Cell Biosensor : Towards Indonesian Food Security</a></p>
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<a class="carousel-control left" href="#slider" data-slide="prev">‹</a>
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<div class="post-box post">
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<h2 class="title">How Dangerous Aflatoxin for Our Bodies?</h2>
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<div class="entry clearfix">
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<div class="featured-image-container">
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<img src="https://static.igem.org/mediawiki/igem.org/b/bf/ITB_Indonesia-sample1.jpg" alt=""/>
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<p>
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Aflatoxins are carcinogenic and mutagenic, heat stable
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    (237-289&deg;C), and can accumulate through the food chain.
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The presence of aflatoxins especially aflatoxin B1 on 
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food such as peanuts...
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</p>
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<div class="readmore">
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<a href="https://2013.igem.org/Team:ITB_Indonesia/BehindTheScene/DangerAflatoxin">Readmore</a>
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</div>
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<div class="post-box post">
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<h2 class="title">Aflatoxin Whole Cell Biosensor : Towards Indonesian Food Security</h2>
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<div class="entry clearfix">
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<img src="https://static.igem.org/mediawiki/igem.org/f/f9/ITB_Indonesia-sample2.jpg" alt=""/>
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<p>
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Not only for our health, Aflatoxin exposure can become
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serious problems for economics and food security in
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certain developing countries. Legumes is still an
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alternative staple food after rice and tubers...
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</p>
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</div>
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<div class="readmore">
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<a href="https://2013.igem.org/Team:ITB_Indonesia/BehindTheScene/AflatoxinWholeCellBiosensor">Readmore</a>
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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:Ehime-Japan"><img src="https://static.igem.org/mediawiki/2012/b/b4/Ecolingual%E3%82%A6%E3%82%A3%E3%82%AD%E7%94%A8%EF%BC%92.png" width="800px"></a></p>
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<h2 class="title">Why Must be Whole Cells Biosensor ?</h2>
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<img src="https://static.igem.org/mediawiki/igem.org/f/f1/ITB_Indonesia-sample3.jpg" alt=""/>
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Accuracy and rapidity in monitoring toxin compounds, pollutants,
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or the presence of a pathogen in the environment became an
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important point from a measuring device (sensor). In the process
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of monitoring a toxin...
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<div class="readmore">
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<a href="https://2013.igem.org/Team:ITB_Indonesia/BehindTheScene/WhyWholeCellBiosensor">Readmore</a>
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<div class="post-box post">
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<h2 class="title">Aflatoxin Whole Biosensor Safetiness</h2>
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<img src="https://static.igem.org/mediawiki/igem.org/8/80/ITB_Indonesia-Sample4.jpg" alt=""/>
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<p>
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The presence  a living cell as biosensor will automatically regulate
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the introduction of a critical environmental point for a biosensor.
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So a whole cell biosensor is more prefered and more advantageous as
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biosensor device...
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<div class="readmore">
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<a href="https://2013.igem.org/Team:ITB_Indonesia/Safety">Readmore</a>
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li> 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>
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<ul>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li>
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                        <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li>
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li>
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li>
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<li><a href="#">Data</a>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li>
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<li><a href="#">Human Practice</a>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li>
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<li><a href="#">Other</a>
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li>
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li> 
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Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know <i>E. coli</i> feelings. It consists of three parts: Main, Connection, and Screen.<br>
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To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
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“E.co-mail” is a communication tool with <i>E. coli</i> and an optical fiber. We would be able to transfer digital information depicted in <i>E.coli</i> through a distance, and probably could send e-mails with this tool. 
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    We tried to reconstruct toppling dominoes with <i>E. coli</i>,
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    and we would be able to draw a movie of firework on LB plate!
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    Main is comprised of two different kinds of <i>E. coli</i> transformants.
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    One is that feels heat and cold and respond differently, producing different
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    signaling molecules. The other catches the signaling molecules and convert
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    the signal into the form of light. As a result, we can see a temperature change
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    around the <i>E. coli</i>. The light signals could be transferred through a distance
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    and be displayed if the Connection and Screen systems work.
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a>
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    It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which
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    we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc…
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    Also, by an optical fiber, the information of those feelings is transmitted
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    to someone in a distant place from a lab. Actually, we focused on <i>E. coli</i>
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    feelings, “hot” and “cold”.
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→  New degradation system
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    We used two different <i>ssrA</i> tags to make a circulatable communication system. One is from
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    <i>M. florum</i> recognized by the Lon protease from <i>M. florum</i>. The other is <i>E. coli ssrA</i> tag (LVA).
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    Because the sequences of those tags are not similar, the degradation systems should function
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→ New parts
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    We made new BioBrick parts!
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  ・BBa_K794000 : This part is cold shock promoter + <i>lasI</i>.
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→ Human Practice
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Ehime-Japan did a lot of projects as Human Practice!
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・Ecreate !
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    We are enjoying gene recombination, and to tell interests of synthetic biology for
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    high school students, we made “E.create”!! It is a card game that people can enjoy
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    building genetic circuits. Also, we did educational activities for high school students
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    and played them with E.create! 
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・Questionnaire
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    Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
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    Thus, we were able to obtain a lot of replies, and found more general opinions
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    about synthetic biology in Japan.
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Ehime-Japan|Home]]
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!align="center"|[[Team:Ehime-Japan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan Official Team Profile]
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!align="center"|[[Team:Ehime-Japan/Project|Project]]
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!align="center"|[[Team:Ehime-Japan/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Ehime-Japan/Modeling|Modeling]]
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!align="center"|[[Team:Ehime-Japan/Notebook|Notebook]]
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!align="center"|[[Team:Ehime-Japan/Safety|Safety]]
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!align="center"|[[Team:Ehime-Japan/Attributions|Attributions]]
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Latest revision as of 01:56, 28 September 2013

How Dangerous Aflatoxin for Our Bodies?

Aflatoxins are carcinogenic and mutagenic, heat stable (237-289°C), and can accumulate through the food chain. The presence of aflatoxins especially aflatoxin B1 on food such as peanuts...

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Aflatoxin Whole Cell Biosensor : Towards Indonesian Food Security

Not only for our health, Aflatoxin exposure can become serious problems for economics and food security in certain developing countries. Legumes is still an alternative staple food after rice and tubers...

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Why Must be Whole Cells Biosensor ?

Accuracy and rapidity in monitoring toxin compounds, pollutants, or the presence of a pathogen in the environment became an important point from a measuring device (sensor). In the process of monitoring a toxin...

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Aflatoxin Whole Biosensor Safetiness

The presence a living cell as biosensor will automatically regulate the introduction of a critical environmental point for a biosensor. So a whole cell biosensor is more prefered and more advantageous as biosensor device...

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