Team:DTU-Denmark/Notebook/2 July 2013

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| Sodium Nitrate || 3
| Sodium Nitrate || 3
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| Sodium Nitrate | 0.3
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| Sodium Nitrate || 0.3
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| Ammonium Acetate | 3000
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| Ammonium Acetate || 3000
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| Ammonium Acetate | 300
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| Ammonium Acetate || 300
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| Ammonium Acetate | 30
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| Ammonium Acetate || 30
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| Ammonium Acetate | 3
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| Ammonium Acetate || 3
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| Sodium Nitrite | 300 (Master)
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| Sodium Nitrite || 300 (Master)
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| Sodium Nitrite | 30
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| Sodium Nitrite || 30
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| Sodium Nitrite | 3
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| Sodium Nitrite || 3
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| Sodium Nitrite | 0.3
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| Sodium Nitrite || 0.3
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| Sodium Nitrite | 0.03
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| Sodium Nitrite || 0.03
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Revision as of 14:50, 2 July 2013

Contents

208 lab

Main purposes today

Create dilution series for biolector experiment.

Weighed:

  • 2.3187g Ammonium acetate
  • 0.2557g Sodium nitrate
  • 0.2080g Sodium nitrite

Diluted each in 10mL M9 medium.

Created dilution series as in https://docs.google.com/spreadsheet/ccc?key=0AkM9Z7voM1otdFZ5SWstOWpURGs2S3M5bElMMllkenc

Salt mM
Sodium Nitrate 300
Sodium Nitrate 30
Sodium Nitrate 3
Sodium Nitrate 0.3
Ammonium Acetate 3000
Ammonium Acetate 300
Ammonium Acetate 30
Ammonium Acetate 3
Sodium Nitrite 300 (Master)
Sodium Nitrite 30
Sodium Nitrite 3
Sodium Nitrite 0.3
Sodium Nitrite 0.03

Who was in the lab

Henrike, Jakob, Kristian, Gosia

Procedure

PCR for 7 fragments:

Conclusion from today

Inconsistent results from the first PCR where obtained with bands in some of the reactions but not in the duplicates. Even the g-Block was not seen though we have easily amplified that before with very bright bands. Something must have gone wrong and that's why we made the additional 34rxns which will be analyzed on gels tomorrow.


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