"
Team:Glendale CC AZ/Protocols/SurivalGrowth
From 2013.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 315: | Line 315: | ||
body { | body { | ||
font-family: "Source Sans Pro"; | font-family: "Source Sans Pro"; | ||
| - | font-size:1. | + | font-size:1.4rem; |
line-height: 2.3rem; | line-height: 2.3rem; | ||
color:#444; | color:#444; | ||
| Line 415: | Line 415: | ||
<div class="content-area"> <article> | <div class="content-area"> <article> | ||
</article> | </article> | ||
| + | <img style="border: 0px solid ; width: 50px; height: 40px;" alt="iGEM" src="http://s21.postimg.org/ff5nkjy9v/IGEM_basic_Logo_stylized.png" align="left"> | ||
<h4>Glendale Community College Arizona<img | <h4>Glendale Community College Arizona<img | ||
style="width: 200px; height: 58px;" alt="GCC" | style="width: 200px; height: 58px;" alt="GCC" | ||
| Line 428: | Line 429: | ||
| - | < | + | <h2><p>Protocols</p></h2><img style="border: 0px solid ; width: 400px; height: 200px;" alt="iGEM" src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" align="right"> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve Assay</a></p> | <a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve Assay</a></p> | ||
| Line 469: | Line 446: | ||
</html> | </html> | ||
| - | == Survival Growth Assay == | + | ==== Survival Growth Assay ==== |
| - | == Materials == | + | ==== Materials ==== |
-P200 micropipette | -P200 micropipette | ||
| Line 483: | Line 460: | ||
| - | == Procedure == | + | ==== Procedure ==== |
1. Label all plates (Keep upside down to avoid condensation on agar) | 1. Label all plates (Keep upside down to avoid condensation on agar) | ||
Latest revision as of 02:34, 28 September 2013
Glendale Community College Arizona
Protocols
Growth Curve Assay
NaCl Growth Curve Assay
Survival Growth Assay
Alkaline Lysis Plasmid Miniprep
Restriction Digest
DNA Isolation
Bioinformatics
Ligation
Transformation
Survival Growth Assay
Materials
-P200 micropipette -10µl bacteria -90µl LB media -Agar plates with appropriate antibiotic -Spreader -20mL ethanol (to sterilize spreader) -Incubator set to 37°C.
Procedure
1. Label all plates (Keep upside down to avoid condensation on agar)
2. Pipette LB media into tube containing bacteria; aspirate.
3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
5. Spread bacteria-LB solution all over plate.
6. Incubate at 37°C for up to 24 hours.
