Team:Glendale CC AZ/Protocols/SurivalGrowth

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== Survival Growth Assay ==
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<img style="border: 0px solid ; width: 50px; height: 40px;" alt="iGEM" src="http://s21.postimg.org/ff5nkjy9v/IGEM_basic_Logo_stylized.png" align="left">
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<h4>Glendale Community College Arizona<img
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style="width: 200px; height: 58px;" alt="GCC"
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  <h2><p>Protocols</p></h2><img style="border: 0px solid ; width: 400px; height: 200px;" alt="iGEM" src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" align="right">
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve  Assay</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/NaCl">NaCl Growth Curve Assay</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth">Survival Growth  Assay</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/RestrictionDigest">Restriction Digest</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/DNAIsolation">DNA Isolation</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Bioinformatics">Bioinformatics</a></p>
 +
<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation">Ligation</a></p>
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Transformation">Transformation</a></p>
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==== Survival Growth Assay ====
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==== Materials ====
     -P200 micropipette
     -P200 micropipette
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     -10 microliters bacteria
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     -10µl bacteria
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     -90 mictroliters LB media
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     -90µl LB media
     -Agar plates with appropriate antibiotic
     -Agar plates with appropriate antibiotic
     -Spreader
     -Spreader
     -20mL ethanol (to sterilize spreader)
     -20mL ethanol (to sterilize spreader)
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     -Incubator set to 37 degrees celcius.
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     -Incubator set to 37°C.
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== Procedure ==
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==== Procedure ====
1. Label all plates (Keep upside down to avoid condensation on agar)
1. Label all plates (Keep upside down to avoid condensation on agar)
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2. Pipette LB media into tube containing bacteria; aspirate.
2. Pipette LB media into tube containing bacteria; aspirate.
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3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate.
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3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
4. Briefly flame the spreader and let cool momentarily.
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5. Spread bacteria-LB solution all over plate.
5. Spread bacteria-LB solution all over plate.
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6. Incubate at 37 degrees celcius for up to 24 hours.
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6. Incubate at 37°C for up to 24 hours.

Latest revision as of 02:34, 28 September 2013

iGEM

Glendale Community College ArizonaGCC

Protocols

iGEM Growth Curve Assay

NaCl Growth Curve Assay

Survival Growth Assay

Alkaline Lysis Plasmid Miniprep

Restriction Digest

DNA Isolation

Bioinformatics

Ligation

Transformation

Survival Growth Assay

Materials 

   -P200 micropipette
   -10µl bacteria
   -90µl LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37°C.


Procedure

1. Label all plates (Keep upside down to avoid condensation on agar)

2. Pipette LB media into tube containing bacteria; aspirate.

3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.

4. Briefly flame the spreader and let cool momentarily.

5. Spread bacteria-LB solution all over plate.

6. Incubate at 37°C for up to 24 hours.

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/SurivalGrowth"