Team:Glendale CC AZ/Protocols/Ligation
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<h4>Glendale Community College Arizona<img | <h4>Glendale Community College Arizona<img | ||
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- | < | + | <h2><p>Protocols</p></h2><img style="border: 0px solid ; width: 400px; height: 200px;" alt="iGEM" src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" align="right"> |
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<a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve Assay</a></p> | <a href="https://2013.igem.org/Team:Glendale_CC_AZ/Protocols/GrowthCurve">Growth Curve Assay</a></p> | ||
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- | + | ==== Ligation Protocol ==== | |
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- | === Ligation Protocol === | + | |
Protocol from: http://parts.igem.org/Help:Protocols/Ligation | Protocol from: http://parts.igem.org/Help:Protocols/Ligation | ||
- | === Materials === | + | ==== Materials ==== |
-digested plasmid backbone | -digested plasmid backbone | ||
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-thermal cycler | -thermal cycler | ||
- | === Procedure === | + | ==== Procedure ==== |
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1. Add 2µL of digested plasmid backbone | 1. Add 2µL of digested plasmid backbone |
Latest revision as of 02:36, 28 September 2013
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Glendale Community College Arizona
Protocols
Ligation Protocol
Protocol from: http://parts.igem.org/Help:Protocols/Ligation
Materials
-digested plasmid backbone -equimolar amount digested Part 1 -equimolar amount digested Part 2 -T4 DNA ligase buffer -T4 DNA ligase -dH2O -thermal cycler
Procedure
1. Add 2µL of digested plasmid backbone
2. Add equimolar amount of digested Part 1
3. Add equimolar amount of digested Part 2
4. Add 1µL T4 DNA ligase buffer.
5. Add 0.5µL T4 DNA ligase.
6. Add water to 10µL.
7. Place in pre-programmed thermal cycler at 16°C for 30 minutes then at 80°C for 20 minutes.