Team:CU-Boulder/Project/Kit/Purification

From 2013.igem.org

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Revision as of 02:54, 28 September 2013

ELPs - Elastin-like Proteins: Elastin-like proteins are simply oligomeric repeats of Val-Pro-Gly-Xaa-Gly (Xaa being any amino acid with the exception of proline). ELPs undergo reversible, inverse phase transitions at at a transition temperature or after the addition of NaCl. Below this temperature/concentration of NaCl, ELPs are soluble. So here at CU-Boulder, we are trying to take advantage of this simple, yet interesting trait of these proteins by attempting to attach these ELPs to proteins in an attempt to come up with an easy and affordable method of protein purification. This method has been proven to work in previous experiments, so we're trying to develop a iGEM part that will include an optimized ELP that will make protein purification a simple process. We designed our ELP to be 100 amino acids long with 10% of the guest residues being tyrosine and 90% being valine. We designed the ELP to solubilize at around 40 degrees Celsius and an NaCl concentration of about .2M. We constructed the ELP because we wanted to create the easiest conditions to induce solubilization. If our method proves successful, then the only required materials to purify an ELP tagged protein would be a heat block and NaCl.

RTX - Repeats in Toxin: RTX is a structural motif consisting of a repeating set of amino acids that allows for precipitation in the presence of calcium. The RTX protein is intrinsically disordered under physiological conditions but undergoes a conformation change upon binding to calcium, otherwise known a ligand-induced disorder-to-order transition, which results in precipitation from solution. Our goal was to take advantage of this characteristic for the purpose of purifying proteins.

RTX tag Characterization

Gel Purification using Color Tags: We have developed a method of protein purification using color tags that allows us to separate proteins on a standard agarose gel.

First, in this time lapse video, AmilCP and dsRED are separated on a .5% agarose gel for 120min

To improve yield, a barrier of standard computer paper is inserted into the path of the protein. This causes the protein to concentrate at the barrier.

Finally, the concentrated protein is cut, frozen, and spun in a homemade minicolumn to extract pure DNA free protein in TAE from the agarose matrix.

Detailed Protocol
Yield and Purity Calculations

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 <dd>Elastin-like proteins are simply oligomeric repeats of Val-Pro-Gly-Xaa-Gly (Xaa being any amino acid with the exception of proline). ELPs undergo reversible, inverse phase transitions at at a transition temperature or after the addition of NaCl. Below this temperature/concentration of NaCl, ELPs are soluble. So here at CU-Boulder, we are trying to take advantage of this simple, yet interesting trait of these proteins by attempting to attach these ELPs to proteins in an attempt to come up with an easy and affordable method of protein purification. This method has been proven to work in previous experiments, so we're trying to develop a iGEM part that will include an optimized ELP that will make protein purification a simple process.
+We designed our ELP to be 100 amino acids long with 10% of the guest residues being tyrosine and 90% being valine. We designed the ELP to solubilize at around 40 degrees Celsius and an NaCl concentration of about .2M. We constructed the ELP because we wanted to create the easiest conditions to induce solubilization. If our method proves successful, then the only required materials to purify an ELP tagged protein would be a heat block and NaCl.
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