Template:Kyoto/Notebook/Sep 28

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(Difference between revisions)
(→Electrophoresis)
(→Colony PCR)
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===Colony PCR===
===Colony PCR===
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<div class="experiment">
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<span class="author"></span>
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{| class="wikitable"
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!Sample||base pair
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|-
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|9/27 pT181 attenuatorC(C3)1||1174
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|-
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|9/27 pT181 attenuatorC(C3)2||1174
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|-
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|9/27 Pcon antisense-DT1||1174
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|-
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|9/27 Pcon antisense-DT2||1174
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|}
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{| class="wikitable"
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!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
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|-
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|5min||30s||30s||24min||
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|}
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</div>

Revision as of 03:31, 28 September 2013

Sep 28

Electrophoresis

Tatsui

LaneSample
1100bp ladder
2Spinach-DT+GAPDH+A1
3Spinach-DT+Spinach+C1
4Spinach-DT+gfp+E1
5Spinach-DT+aptamer+G1
6Pcon-tetR aptamer-DT+GADPH+A3
7Pcon-tetR aptamer-DT+Spinach+C3
8Pcon-tetR aptamer-DT+gfp+E3
9Pcon-tetR aptamer-DT+aptamer+G3
10Pcon-RBS-GFP-DT+GADPH+A5
11Pcon-RBS-GFP-DT+Spinach+C5
12Pcon-RBS-GFP-DT+gfp+E5
13Pcon-RBS-GFP-DT+aptamer+G5
14100bp ladder

File:Igku xxxxxx.xxx

LaneSample
1Pcon-antisense-Spinach-DT+GAPDH+A7
2Pcon-antisense-Spinach-DT+Spinach+C7
3Pcon-antisense-Spinach-DT+gfp+E7
4Pcon-antisense-Spinach-DT+aptamer+G7
5100bp ladder
6Pcon-Spinach-DT+GAPDH+A9
7Pcon-Spinach-DT+Spinach+C9
8Pcon-Spinach-DT+gfp+E9
9Pcon-Spinach-DT+aptamer+G9

File:Igku xxxxxx.xxx

Colony PCR

Samplebase pair
9/27 pT181 attenuatorC(C3)11174
9/27 pT181 attenuatorC(C3)21174
9/27 Pcon antisense-DT11174
9/27 Pcon antisense-DT21174
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s24min
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