Team:NJU NJUT China

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<body>
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<img src="https://static.igem.org/mediawiki/igem.org/e/ee/NJU_NJUT_CHINA_Bg1.jpg" style="width:100%; height:auto"/>
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+
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<!-- end #header -->
+
-
        <ul id="navigation">
+
-
            <li class="home" style="width: 100px;"><a href=""><span>Home</span></a></li>
+
-
            <li class="about" style="width: 115px;"><a href="" style="width:115px;"><span>TEAM</span></a></li>
+
-
            <li class="search" style="width: 135px;"><a href="" style="width:135px;"><span>PROJECT</span></a></li>
+
-
            <li class="photos" style="width: 150px;"><a href="" style="width:150px;"><span>MODELING</span></a></li>
+
-
            <li class="rssfeed" style="width: 115px;"><a href="" style="width:115px;"><span>DATA</span></a></li>
+
-
            <li class="podcasts" style="width: 125px;"><a href=""style="width:125px;"><span>SAFETY</span></a></li>
+
-
            <li class="contact" style="width: 205px;"><a href=""style="width:205px;"><span>HUMANPRACTICE</span></a></li>
+
-
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<li><img src="http://igem.3vfree.us/images/5.jpg" alt="Fifth Image" /></li>
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<li><img src="http://igem.3vfree.us/images/6.jpg" alt="Sixth Image" /></li>
+
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<span class="pxs_prev"></span>
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-
<li><img src="http://igem.3vfree.us/images/thumbs/6.jpg" alt="Sixth Image" /></li>
+
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+
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+
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-
    <div style="clear: both;">&nbsp;</div>
+
<center>
-
<!-- end #menu -->
+
<div id="guide"><img src="https://static.igem.org/mediawiki/igem.org/3/3c/NJU_NJUT_CHINA_Guide1.png" usemap="#Map"  style="width:100%"/>
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<!--焦点图-->
 +
<div class="film_focus">
 +
 
 +
<div class="film_focus_imgs_wrap">
 +
<ul class="film_focus_imgs">
 +
<li><a target="_blank" href="#"><img width="722" height="310" src="https://static.igem.org/mediawiki/igem.org/3/35/NJU_NJUT_CHINA_Pic1.jpg" alt="TEAM PHOTO" /></a></li>
 +
<li><a target="_blank" href="#"><img width="722" height="310" src="https://static.igem.org/mediawiki/igem.org/4/47/NJU_NJUT_CHINA_Pic2.jpg" alt="CRISPR/Cas " /></a></li>
 +
<li><a target="_blank" href="#"><img width="722" height="310" src="https://static.igem.org/mediawiki/igem.org/1/1c/NJU_NJUT_CHINA_Pic3.jpg" alt="PCR product" /></a></li>
 +
<li><a target="_blank" href="#"><img width="722" height="310" src="https://static.igem.org/mediawiki/igem.org/b/b6/MG_0042.JPG" alt="Doing the experiment" /></a></li>
 +
</ul>
 +
</div>
 +
<div class="film_focus_desc">
 +
<h3>photo introduction</h3>
 +
<ul class="film_focus_nav">
 +
<li class="cur">
 +
<a target="_blank" ><img width="83" height="55" src="https://static.igem.org/mediawiki/igem.org/9/90/NJU_NJUT_CHINA_S1.jpg" alt="" /></a>
 +
<p align="left" style="font-size:15px">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;TEAM</p>
 +
</li>
 +
<li>
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<a target="_blank" ><img width="83" height="55" src="https://static.igem.org/mediawiki/igem.org/1/12/NJU_NJUT_CHINA_S2.jpg" alt="" /></a>
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<p align="left" style="font-size:15px">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;CRISPR</p>
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<a target="_blank" ><img width="83" height="55" src="https://static.igem.org/mediawiki/igem.org/d/dd/NJU_NJUT_CHINA_S3.jpg" alt="" /></a>
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                <p align="left" style="font-size:15px">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;PCR
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<a target="_blank" ><img width="83" height="55" src="https://static.igem.org/mediawiki/igem.org/b/b6/MG_0042.JPG" alt="" /></a>
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<p align="left"style="font-size:15px">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;experiment</p>
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  <h2 class="title"><a href="#" >Project  </a></h2>
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<br>
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<p> &nbsp;&nbsp;&nbsp;&nbsp;Most of the bacteria and archaea acquire virus resistance by integrating short viral nucleotide acid fragments into the clusters of regularly interspaced short palindromic repeats (CRISPRs). <img src="http://igem.3vfree.us/images/ind1.jpg" width="212" height="164"  width="237" align='right'/> And CRISPR-based defense system can also protects them against the invading DNA and/or RNA elements. It is believed that the integrated CRISPR sequences have the ability to form a genetic memory which prevents the host from being infected. The CRISPRs and Cas (CRISPR-associated) interact and form this prokaryotic adaptive immune system....</p>
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<p align="left" style="margin-left:5px; margin-right:5px;"> &nbsp;&nbsp;&nbsp;&nbsp;All cellular systems evolve ways to combat predators and genomic parasites. In bacteria and archaea, numerous resistance mechanisms have been developed against phage.  
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<p>&nbsp;</p>
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<img src="https://static.igem.org/mediawiki/igem.org/d/de/NJU_NJUT_CHINA_Ind1.jpg" width="212" height="164"  width="237" align='right'/>Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) adaptive immune systems are found in bacteria and archaea to protect the hosts against the invasion of viruses and plasmids. Most of the bacteria and archaea acquire virus resistance by integrating short viral nucleotide acid fragments into the CRISPR sequences.....
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  <h2 class="title"><a href="#">Introduction to our experiment</a></h2>
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  <a href="https://2013.igem.org/Team:NJU_NJUT_China/team"><img src="https://static.igem.org/mediawiki/igem.org/9/9f/NJU_NJUT_CHINA_Teamtitle.jpg" width="80" height="201" /></a></div>
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<p align="left" style=" margin:3px 8px 3px 8px; text-shadow:#00FF00">Combing the outstanding students from Nanjing University and Nanjing University of Technology, we are trying to make the CRISPR/Cas systems work in the eukaryote cell with the help of the synthetic biology methods. Furthermore, considering the spacers’ sequence as the result of evolution, our team is developing a highly-probable algorithm to direct the synthesis and seeking of spacers or proto-spacers. </p>
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<p>Based on the fundamental synthetic biology methods, include the molecular cloning, long PCR, recombinant construction, restriction digestion and yeast transformation, our project aims to enable  Saccharomyces cerevisiae to resist viruses utilizing the CRISPR system found in most bacteria and archaea lately. </p>
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  </div>
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<p>To ensure the accurate output, we divide the original linear gene into several elements , and also purify and propagate the complete target sequence to make the both two models work in the host cell. To present the actual effect of CRISPR in yeast, we design our own mimic virus construction based on the homologous sequences shown in the specific viral genome database, which can be recognized, and control the baseline both for anti-virus ability and CRISPR's effect in its original form.</p>
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<div id="contentD">
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                    <p>More specifically, we first obtain our CRISPR system by genome-extraction from E.coli str W3110, and clone the respective target genes as modules. The construction, as well as the mimic virus construction, IS made by restriction-ligase way. We transform the mimic virus plasmid first by lithium acetate method and verify the availability by several antibiotics. We then import CRISPR system in the same way, utilizing the Ochre mutation and GFP switch-off to evaluate the anti-virus feasibility.</p>
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  <p align="left" style="font-size:16px">support</p>
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  <p>Thanks to Dr.Xingxu Huang for his kind support in supplying us the cas9 gene.</p>
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      <p> Thanks to KeyGene Biotech for its help in providing technical support.</p>
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  <p> Thanks to Fundamental Biological Experiment Education Center（南大生科教学实验中心） for its support in providing the equipment. </p>
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  <p>Attributions:</p>
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<p>Ruitao Jin Min Xi</p>
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<p>NJUT Ning Meng Xiali Luan Jie Tang Sitong He</p>
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parentNode:e.removeChild(e.appendChild(v.createElement("div"))).parentNode===null,deleteExpando:true,checkClone:false,scriptEval:false,noCloneEvent:true,boxModel:null};b.type="text/javascript";try{b.appendChild(v.createTextNode("window."+g+"=1;"))}catch(k){}a.insertBefore(b,a.firstChild);if(p[g]){c.support.scriptEval=true;delete p[g]}try{delete b.test}catch(s){c.support.deleteExpando=false}a.removeChild(b);if(e.attachEvent&&e.fireEvent){e.attachEvent("onclick",function m(){c.support.noCloneEvent=
-
$thumbs = $pxs_thumbnails.children(),
+
false;e.detachEvent("onclick",m)});e.cloneNode(true).fireEvent("onclick")}e=v.createElement("div");e.innerHTML="<input type='radio' name='radiotest' checked='checked'/>";a=v.createDocumentFragment();a.appendChild(e.firstChild);c.support.checkClone=a.cloneNode(true).cloneNode(true).lastChild.checked;c(function(){var m=v.createElement("div");m.style.width=m.style.paddingLeft="1px";v.body.appendChild(m);c.boxModel=c.support.boxModel=m.offsetWidth===2;v.body.removeChild(m).style.display="none"});a=function(m){var r=
-
//the interval for the autoplay mode
+
v.createElement("div");m="on"+m;var y=m in r;if(!y){r.setAttribute(m,"return;");y=typeof r[m]==="function"}return y};c.support.submitBubbles=a("submit");c.support.changeBubbles=a("change");a=b=e=f=l=null}})();c.props={"for":"htmlFor","class":"className",readonly:"readOnly",maxlength:"maxLength",cellspacing:"cellSpacing",rowspan:"rowSpan",colspan:"colSpan",tabindex:"tabIndex",usemap:"useMap",frameborder:"frameBorder"};var W="jQuery"+z(),Za=0,Ha={};c.extend({cache:{},expando:W,noData:{embed:true,object:true,
-
slideshow,
+
applet:true},data:function(a,b,e){if(!(a.nodeName&&c.noData[a.nodeName.toLowerCase()])){a=a==p?Ha:a;var g=a[W],f=c.cache;if(!g&&typeof b==="string"&&e===j)return null;g||(g=++Za);if(typeof b==="object"){a[W]=g;f[g]=c.extend(true,{},b)}else if(!f[g]){a[W]=g;f[g]={}}a=f[g];if(e!==j)a[b]=e;return typeof b==="string"?a[b]:a}},removeData:function(a,b){if(!(a.nodeName&&c.noData[a.nodeName.toLowerCase()])){a=a==p?Ha:a;var e=a[W],g=c.cache,f=g[e];if(b){if(f){delete f[b];c.isEmptyObject(f)&&c.removeData(a)}}else{if(c.support.deleteExpando)delete a[c.expando];
-
//the loading image
+
else a.removeAttribute&&a.removeAttribute(c.expando);delete g[e]}}}});c.fn.extend({data:function(a,b){if(typeof a==="undefined"&&this.length)return c.data(this[0]);else if(typeof a==="object")return this.each(function(){c.data(this,a)});var e=a.split(".");e[1]=e[1]?"."+e[1]:"";if(b===j){var g=this.triggerHandler("getData"+e[1]+"!",[e[0]]);if(g===j&&this.length)g=c.data(this[0],a);return g===j&&e[1]?this.data(e[0]):g}else return this.trigger("setData"+e[1]+"!",[e[0],b]).each(function(){c.data(this,
-
$pxs_loading = $('.pxs_loading',$pxs_container),
+
a,b)})},removeData:function(a){return this.each(function(){c.removeData(this,a)})}});c.extend({queue:function(a,b,e){if(a){b=(b||"fx")+"queue";var g=c.data(a,b);if(!e)return g||[];if(!g||c.isArray(e))g=c.data(a,b,c.makeArray(e));else g.push(e);return g}},dequeue:function(a,b){b=b||"fx";var e=c.queue(a,b),g=e.shift();if(g==="inprogress")g=e.shift();if(g){b==="fx"&&e.unshift("inprogress");g.call(a,function(){c.dequeue(a,b)})}}});c.fn.extend({queue:function(a,b){if(typeof a!=="string"){b=a;a="fx"}if(b===
-
$pxs_slider_wrapper = $('.pxs_slider_wrapper',$pxs_container);
+
j)return c.queue(this[0],a);return this.each(function(){var e=c.queue(this,a,b);a==="fx"&&e[0]!=="inprogress"&&c.dequeue(this,a)})},dequeue:function(a){return this.each(function(){c.dequeue(this,a)})},delay:function(a,b){a=c.fx?c.fx.speeds[a]||a:a;b=b||"fx";return this.queue(b,function(){var e=this;setTimeout(function(){c.dequeue(e,b)},a)})},clearQueue:function(a){return this.queue(a||"fx",[])}});var Ia=/[\n\t]/g,sa=/\s+/,$a=/\r/g,ab=/href|src|style/,bb=/(button|input)/i,cb=/(button|input|object|select|textarea)/i,
-
+
db=/^(a|area)$/i,Ja=/radio|checkbox/;c.fn.extend({attr:function(a,b){return o(this,a,b,true,c.attr)},removeAttr:function(a){return this.each(function(){c.attr(this,a,"");this.nodeType===1&&this.removeAttribute(a)})},addClass:function(a){if(c.isFunction(a))return this.each(function(r){var y=c(this);y.addClass(a.call(this,r,y.attr("class")))});if(a&&typeof a==="string")for(var b=(a||"").split(sa),e=0,g=this.length;e<g;e++){var f=this[e];if(f.nodeType===1)if(f.className){for(var l=" "+f.className+" ",
-
//first preload all the images
+
k=f.className,s=0,m=b.length;s<m;s++)if(l.indexOf(" "+b[s]+" ")<0)k+=" "+b[s];f.className=c.trim(k)}else f.className=a}return this},removeClass:function(a){if(c.isFunction(a))return this.each(function(m){var r=c(this);r.removeClass(a.call(this,m,r.attr("class")))});if(a&&typeof a==="string"||a===j)for(var b=(a||"").split(sa),e=0,g=this.length;e<g;e++){var f=this[e];if(f.nodeType===1&&f.className)if(a){for(var l=(" "+f.className+" ").replace(Ia," "),k=0,s=b.length;k<s;k++)l=l.replace(" "+b[k]+" ",
-
var loaded = 0,
+
" ");f.className=c.trim(l)}else f.className=""}return this},toggleClass:function(a,b){var e=typeof a,g=typeof b==="boolean";if(c.isFunction(a))return this.each(function(f){var l=c(this);l.toggleClass(a.call(this,f,l.attr("class"),b),b)});return this.each(function(){if(e==="string")for(var f,l=0,k=c(this),s=b,m=a.split(sa);f=m[l++];){s=g?s:!k.hasClass(f);k[s?"addClass":"removeClass"](f)}else if(e==="undefined"||e==="boolean"){this.className&&c.data(this,"__className__",this.className);this.className=
-
$images = $pxs_slider_wrapper.find('img');
+
this.className||a===false?"":c.data(this,"__className__")||""}})},hasClass:function(a){a=" "+a+" ";for(var b=0,e=this.length;b<e;b++)if((" "+this[b].className+" ").replace(Ia," ").indexOf(a)>-1)return true;return false},val:function(a){if(a===j){var b=this[0];if(b){if(c.nodeName(b,"option"))return(b.attributes.value||{}).specified?b.value:b.text;if(c.nodeName(b,"select")){var e=b.selectedIndex,g=[],f=b.options;b=b.type==="select-one";if(e<0)return null;var l=b?e:0;for(e=b?e+1:f.length;l<e;l++){var k=
-
+
f[l];if(k.selected){a=c(k).val();if(b)return a;g.push(a)}}return g}if(Ja.test(b.type)&&!c.support.checkOn)return b.getAttribute("value")===null?"on":b.value;return(b.value||"").replace($a,"")}return j}var s=c.isFunction(a);return this.each(function(m){var r=c(this),y=a;if(this.nodeType===1){if(s)y=a.call(this,m,r.val());if(typeof y==="number")y+="";if(c.isArray(y)&&Ja.test(this.type))this.checked=c.inArray(r.val(),y)>=0;else if(c.nodeName(this,"select")){var B=c.makeArray(y);c("option",this).each(function(){this.selected=
-
$images.each(function(){
+
c.inArray(c(this).val(),B)>=0});if(!B.length)this.selectedIndex=-1}else this.value=y}})}});c.extend({attrFn:{val:true,css:true,html:true,text:true,data:true,width:true,height:true,offset:true},attr:function(a,b,e,g){if(!a||a.nodeType===3||a.nodeType===8)return j;if(g&&b in c.attrFn)return c(a)[b](e);g=a.nodeType!==1||!c.isXMLDoc(a);var f=e!==j;b=g&&c.props[b]||b;if(a.nodeType===1){var l=ab.test(b);if(b in a&&g&&!l){if(f){b==="type"&&bb.test(a.nodeName)&&a.parentNode&&c.error("type property can't be changed");
-
var $img = $(this);
+
a[b]=e}if(c.nodeName(a,"form")&&a.getAttributeNode(b))return a.getAttributeNode(b).nodeValue;if(b==="tabIndex")return(b=a.getAttributeNode("tabIndex"))&&b.specified?b.value:cb.test(a.nodeName)||db.test(a.nodeName)&&a.href?0:j;return a[b]}if(!c.support.style&&g&&b==="style"){if(f)a.style.cssText=""+e;return a.style.cssText}f&&a.setAttribute(b,""+e);a=!c.support.hrefNormalized&&g&&l?a.getAttribute(b,2):a.getAttribute(b);return a===null?j:a}return c.style(a,b,e)}});var da=/\.(.*)$/,eb=function(a){return a.replace(/[^\w\s\.\|`]/g,
-
$('<img/>').load(function(){
+
function(b){return"\\"+b})};c.event={add:function(a,b,e,g){if(!(a.nodeType===3||a.nodeType===8)){if(a.setInterval&&a!==p&&!a.frameElement)a=p;var f,l;if(e.handler){f=e;e=f.handler}if(!e.guid)e.guid=c.guid++;if(l=c.data(a)){var k=l.events=l.events||{},s=l.handle;if(!s)l.handle=s=function(){return typeof c!=="undefined"&&!c.event.triggered?c.event.handle.apply(s.elem,arguments):j};s.elem=a;b=b.split(" ");for(var m,r=0,y;m=b[r++];){l=f?c.extend({},f):{handler:e,data:g};if(m.indexOf(".")>-1){y=m.split(".");
-
++loaded;
+
m=y.shift();l.namespace=y.slice(0).sort().join(".")}else{y=[];l.namespace=""}l.type=m;l.guid=e.guid;var B=k[m],F=c.event.special[m]||{};if(!B){B=k[m]=[];if(!F.setup||F.setup.call(a,g,y,s)===false)if(a.addEventListener)a.addEventListener(m,s,false);else a.attachEvent&&a.attachEvent("on"+m,s)}if(F.add){F.add.call(a,l);if(!l.handler.guid)l.handler.guid=e.guid}B.push(l);c.event.global[m]=true}a=null}}},global:{},remove:function(a,b,e,g){if(!(a.nodeType===3||a.nodeType===8)){var f,l=0,k,s,m,r,y,B,F=c.data(a),
-
if(loaded == total_elems*2){
+
I=F&&F.events;if(F&&I){if(b&&b.type){e=b.handler;b=b.type}if(!b||typeof b==="string"&&b.charAt(0)==="."){b=b||"";for(f in I)c.event.remove(a,f+b)}else{for(b=b.split(" ");f=b[l++];){r=f;k=f.indexOf(".")<0;s=[];if(!k){s=f.split(".");f=s.shift();m=new RegExp("(^|\\.)"+c.map(s.slice(0).sort(),eb).join("\\.(?:.*\\.)?")+"(\\.|$)")}if(y=I[f])if(e){r=c.event.special[f]||{};for(H=g||0;H<y.length;H++){B=y[H];if(e.guid===B.guid){if(k||m.test(B.namespace)){g==null&&y.splice(H--,1);r.remove&&r.remove.call(a,B)}if(g!=
-
$pxs_loading.hide();
+
null)break}}if(y.length===0||g!=null&&y.length===1){if(!r.teardown||r.teardown.call(a,s)===false)Ka(a,f,F.handle);delete I[f]}}else for(var H=0;H<y.length;H++){B=y[H];if(k||m.test(B.namespace)){c.event.remove(a,r,B.handler,H);y.splice(H--,1)}}}if(c.isEmptyObject(I)){if(b=F.handle)b.elem=null;delete F.events;delete F.handle;c.isEmptyObject(F)&&c.removeData(a)}}}}},trigger:function(a,b,e,g){var f=a.type||a;if(!g){a=typeof a==="object"?a[W]?a:c.extend(c.Event(f),a):c.Event(f);if(f.indexOf("!")>=0){a.type=
-
$pxs_slider_wrapper.show();
+
f=f.slice(0,-1);a.exclusive=true}if(!e){a.stopPropagation();c.event.global[f]&&c.each(c.cache,function(){this.events&&this.events[f]&&c.event.trigger(a,b,this.handle.elem)})}if(!e||e.nodeType===3||e.nodeType===8)return j;a.result=j;a.target=e;b=c.makeArray(b);b.unshift(a)}a.currentTarget=e;(g=c.data(e,"handle"))&&g.apply(e,b);g=e.parentNode||e.ownerDocument;try{if(!(e&&e.nodeName&&c.noData[e.nodeName.toLowerCase()]))if(e["on"+f]&&e["on"+f].apply(e,b)===false)a.result=false}catch(l){}if(!a.isPropagationStopped()&&
-
+
g)c.event.trigger(a,b,g,true);else if(!a.isDefaultPrevented()){g=a.target;var k,s=c.nodeName(g,"a")&&f==="click",m=c.event.special[f]||{};if((!m._default||m._default.call(e,a)===false)&&!s&&!(g&&g.nodeName&&c.noData[g.nodeName.toLowerCase()])){try{if(g[f]){if(k=g["on"+f])g["on"+f]=null;c.event.triggered=true;g[f]()}}catch(r){}if(k)g["on"+f]=k;c.event.triggered=false}}},handle:function(a){var b,e,g,f;a=arguments[0]=c.event.fix(a||p.event);a.currentTarget=this;b=a.type.indexOf(".")<0&&!a.exclusive;
-
//one images width (assuming all images have the same sizes)
+
if(!b){e=a.type.split(".");a.type=e.shift();g=new RegExp("(^|\\.)"+e.slice(0).sort().join("\\.(?:.*\\.)?")+"(\\.|$)")}f=c.data(this,"events");e=f[a.type];if(f&&e){e=e.slice(0);f=0;for(var l=e.length;f<l;f++){var k=e[f];if(b||g.test(k.namespace)){a.handler=k.handler;a.data=k.data;a.handleObj=k;k=k.handler.apply(this,arguments);if(k!==j){a.result=k;if(k===false){a.preventDefault();a.stopPropagation()}}if(a.isImmediatePropagationStopped())break}}}return a.result},props:"altKey attrChange attrName bubbles button cancelable charCode clientX clientY ctrlKey currentTarget data detail eventPhase fromElement handler keyCode layerX layerY metaKey newValue offsetX offsetY originalTarget pageX pageY prevValue relatedNode relatedTarget screenX screenY shiftKey srcElement target toElement view wheelDelta which".split(" "),
-
var one_image_w = $pxs_slider.find('img:first').width();
+
fix:function(a){if(a[W])return a;var b=a;a=c.Event(b);for(var e=this.props.length,g;e;){g=this.props[--e];a[g]=b[g]}if(!a.target)a.target=a.srcElement||v;if(a.target.nodeType===3)a.target=a.target.parentNode;if(!a.relatedTarget&&a.fromElement)a.relatedTarget=a.fromElement===a.target?a.toElement:a.fromElement;if(a.pageX==null&&a.clientX!=null){b=v.documentElement;e=v.body;a.pageX=a.clientX+(b&&b.scrollLeft||e&&e.scrollLeft||0)-(b&&b.clientLeft||e&&e.clientLeft||0);a.pageY=a.clientY+(b&&b.scrollTop||
-
+
e&&e.scrollTop||0)-(b&&b.clientTop||e&&e.clientTop||0)}if(!a.which&&(a.charCode||a.charCode===0?a.charCode:a.keyCode))a.which=a.charCode||a.keyCode;if(!a.metaKey&&a.ctrlKey)a.metaKey=a.ctrlKey;if(!a.which&&a.button!==j)a.which=a.button&1?1:a.button&2?3:a.button&4?2:0;return a},guid:1E8,proxy:c.proxy,special:{ready:{setup:c.bindReady,teardown:c.noop},live:{add:function(a){c.event.add(this,a.origType,c.extend({},a,{handler:S}))},remove:function(a){var b=true,e=a.origType.replace(da,"");c.each(c.data(this,
-
/*
+
"events").live||[],function(){if(e===this.origType.replace(da,""))return b=false});b&&c.event.remove(this,a.origType,S)}},beforeunload:{setup:function(a,b,e){if(this.setInterval)this.onbeforeunload=e;return false},teardown:function(a,b){if(this.onbeforeunload===b)this.onbeforeunload=null}}}};var Ka=v.removeEventListener?function(a,b,e){a.removeEventListener(b,e,false)}:function(a,b,e){a.detachEvent("on"+b,e)};c.Event=function(a){if(!this.preventDefault)return new c.Event(a);if(a&&a.type){this.originalEvent=
-
need to set width of the slider,
+
a;this.type=a.type}else this.type=a;this.timeStamp=z();this[W]=true};c.Event.prototype={preventDefault:function(){this.isDefaultPrevented=U;var a=this.originalEvent;if(a){a.preventDefault&&a.preventDefault();a.returnValue=false}},stopPropagation:function(){this.isPropagationStopped=U;var a=this.originalEvent;if(a){a.stopPropagation&&a.stopPropagation();a.cancelBubble=true}},stopImmediatePropagation:function(){this.isImmediatePropagationStopped=U;this.stopPropagation()},isDefaultPrevented:G,isPropagationStopped:G,
-
of each one of its elements, and of the
+
isImmediatePropagationStopped:G};var La=function(a){var b=a.relatedTarget;try{for(;b&&b!==this;)b=b.parentNode;if(b!==this){a.type=a.data;c.event.handle.apply(this,arguments)}}catch(e){}},Ma=function(a){a.type=a.data;c.event.handle.apply(this,arguments)};c.each({mouseenter:"mouseover",mouseleave:"mouseout"},function(a,b){c.event.special[a]={setup:function(e){c.event.add(this,b,e&&e.selector?Ma:La,a)},teardown:function(e){c.event.remove(this,b,e&&e.selector?Ma:La)}}});if(!c.support.submitBubbles)c.event.special.submit=
-
navigation buttons
+
{setup:function(){if(this.nodeName.toLowerCase()!=="form"){c.event.add(this,"click.specialSubmit",function(a){var b=a.target,e=b.type;if((e==="submit"||e==="image")&&c(b).closest("form").length)return R("submit",this,arguments)});c.event.add(this,"keypress.specialSubmit",function(a){var b=a.target,e=b.type;if((e==="text"||e==="password")&&c(b).closest("form").length&&a.keyCode===13)return R("submit",this,arguments)})}else return false},teardown:function(){c.event.remove(this,".specialSubmit")}};if(!c.support.changeBubbles){var ta=
-
*/
+
/textarea|input|select/i,ua,Na=function(a){var b=a.type,e=a.value;if(b==="radio"||b==="checkbox")e=a.checked;else if(b==="select-multiple")e=a.selectedIndex>-1?c.map(a.options,function(g){return g.selected}).join("-"):"";else if(a.nodeName.toLowerCase()==="select")e=a.selectedIndex;return e},va=function(a,b){var e=a.target,g,f;if(!(!ta.test(e.nodeName)||e.readOnly)){g=c.data(e,"_change_data");f=Na(e);if(a.type!=="focusout"||e.type!=="radio")c.data(e,"_change_data",f);if(!(g===j||f===g))if(g!=null||
-
setWidths($pxs_slider,
+
f){a.type="change";return c.event.trigger(a,b,e)}}};c.event.special.change={filters:{focusout:va,click:function(a){var b=a.target,e=b.type;if(e==="radio"||e==="checkbox"||b.nodeName.toLowerCase()==="select")return va.call(this,a)},keydown:function(a){var b=a.target,e=b.type;if(a.keyCode===13&&b.nodeName.toLowerCase()!=="textarea"||a.keyCode===32&&(e==="checkbox"||e==="radio")||e==="select-multiple")return va.call(this,a)},beforeactivate:function(a){a=a.target;c.data(a,"_change_data",Na(a))}},setup:function(){if(this.type===
-
$elems,
+
"file")return false;for(var a in ua)c.event.add(this,a+".specialChange",ua[a]);return ta.test(this.nodeName)},teardown:function(){c.event.remove(this,".specialChange");return ta.test(this.nodeName)}};ua=c.event.special.change.filters}v.addEventListener&&c.each({focus:"focusin",blur:"focusout"},function(a,b){function e(g){g=c.event.fix(g);g.type=b;return c.event.handle.call(this,g)}c.event.special[b]={setup:function(){this.addEventListener(a,e,true)},teardown:function(){this.removeEventListener(a,
-
total_elems,
+
e,true)}}});c.each(["bind","one"],function(a,b){c.fn[b]=function(e,g,f){if(typeof e==="object"){for(var l in e)this[b](l,g,e[l],f);return this}if(c.isFunction(g)){f=g;g=j}var k=b==="one"?c.proxy(f,function(m){c(this).unbind(m,k);return f.apply(this,arguments)}):f;if(e==="unload"&&b!=="one")this.one(e,g,f);else{l=0;for(var s=this.length;l<s;l++)c.event.add(this[l],e,k,g)}return this}});c.fn.extend({unbind:function(a,b){if(typeof a==="object"&&!a.preventDefault)for(var e in a)this.unbind(e,a[e]);else{e=
-
$pxs_bg1,
+
0;for(var g=this.length;e<g;e++)c.event.remove(this[e],a,b)}return this},delegate:function(a,b,e,g){return this.live(b,e,g,a)},undelegate:function(a,b,e){return arguments.length===0?this.unbind("live"):this.die(b,null,e,a)},trigger:function(a,b){return this.each(function(){c.event.trigger(a,b,this)})},triggerHandler:function(a,b){if(this[0]){a=c.Event(a);a.preventDefault();a.stopPropagation();c.event.trigger(a,b,this[0]);return a.result}},toggle:function(a){for(var b=arguments,e=1;e<b.length;)c.proxy(a,
-
$pxs_bg2,
+
b[e++]);return this.click(c.proxy(a,function(g){var f=(c.data(this,"lastToggle"+a.guid)||0)%e;c.data(this,"lastToggle"+a.guid,f+1);g.preventDefault();return b[f].apply(this,arguments)||false}))},hover:function(a,b){return this.mouseenter(a).mouseleave(b||a)}});var Oa={focus:"focusin",blur:"focusout",mouseenter:"mouseover",mouseleave:"mouseout"};c.each(["live","die"],function(a,b){c.fn[b]=function(e,g,f,l){var k,s=0,m,r,y=l||this.selector,B=l?this:c(this.context);if(c.isFunction(g)){f=g;g=j}for(e=
-
$pxs_bg3,
+
(e||"").split(" ");(k=e[s++])!=null;){l=da.exec(k);m="";if(l){m=l[0];k=k.replace(da,"")}if(k==="hover")e.push("mouseenter"+m,"mouseleave"+m);else{r=k;if(k==="focus"||k==="blur"){e.push(Oa[k]+m);k+=m}else k=(Oa[k]||k)+m;b==="live"?B.each(function(){c.event.add(this,L(k,y),{data:g,selector:y,handler:f,origType:k,origHandler:f,preType:r})}):B.unbind(L(k,y),f)}}return this}});c.each("blur focus focusin focusout load resize scroll unload click dblclick mousedown mouseup mousemove mouseover mouseout mouseenter mouseleave change select submit keydown keypress keyup error".split(" "),
-
one_image_w,
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this[a].style.display=e||"";if(c.css(this[a],"display")==="none"){e=this[a].nodeName;var g;if(Ba[e])g=Ba[e];else{var f=c("<"+e+" />").appendTo("body");g=f.css("display");if(g==="none")g="block";f.remove();Ba[e]=g}c.data(this[a],"olddisplay",g)}}a=0;for(b=this.length;a<b;a++)this[a].style.display=c.data(this[a],"olddisplay")||"";return this}},hide:function(a,b){if(a||a===0)return this.animate(T("hide",3),a,b);else{a=0;for(b=this.length;a<b;a++){var e=c.data(this[a],"olddisplay");!e&&e!=="none"&&c.data(this[a],
-
highlight($thumbs.eq(current));
+
"olddisplay",c.css(this[a],"display"))}a=0;for(b=this.length;a<b;a++)this[a].style.display="none";return this}},_toggle:c.fn.toggle,toggle:function(a,b){var e=typeof a==="boolean";if(c.isFunction(a)&&c.isFunction(b))this._toggle.apply(this,arguments);else a==null||e?this.each(function(){var g=e?a:c(this).is(":hidden");c(this)[g?"show":"hide"]()}):this.animate(T("toggle",3),a,b);return this},fadeTo:function(a,b,e){return this.filter(":hidden").css("opacity",0).show().end().animate({opacity:b},a,e)},
-
slide(current,
+
animate:function(a,b,e,g){var f=c.speed(b,e,g);if(c.isEmptyObject(a))return this.each(f.complete);return this[f.queue===false?"each":"queue"](function(){var l=c.extend({},f),k,s=this.nodeType===1&&c(this).is(":hidden"),m=this;for(k in a){var r=k.replace(ya,za);if(k!==r){a[r]=a[k];delete a[k];k=r}if(a[k]==="hide"&&s||a[k]==="show"&&!s)return l.complete.call(this);if((k==="height"||k==="width")&&this.style){l.display=c.css(this,"display");l.overflow=this.style.overflow}if(c.isArray(a[k])){(l.specialEasing=
-
$pxs_slider,
+
l.specialEasing||{})[k]=a[k][1];a[k]=a[k][0]}}if(l.overflow!=null)this.style.overflow="hidden";l.curAnim=c.extend({},a);c.each(a,function(y,B){var F=new c.fx(m,l,y);if(Bb.test(B))F[B==="toggle"?s?"show":"hide":B](a);else{var I=Cb.exec(B),H=F.cur(true)||0;if(I){B=parseFloat(I[2]);var P=I[3]||"px";if(P!=="px"){m.style[y]=(B||1)+P;H=(B||1)/F.cur(true)*H;m.style[y]=H+P}if(I[1])B=(I[1]==="-="?-1:1)*B+H;F.custom(H,B,P)}else F.custom(H,B,"")}});return true})},stop:function(a,b){var e=c.timers;a&&this.queue([]);
-
$pxs_bg3,
+
this.each(function(){for(var g=e.length-1;g>=0;g--)if(e[g].elem===this){b&&e[g](true);e.splice(g,1)}});b||this.dequeue();return this}});c.each({slideDown:T("show",1),slideUp:T("hide",1),slideToggle:T("toggle",1),fadeIn:{opacity:"show"},fadeOut:{opacity:"hide"}},function(a,b){c.fn[a]=function(e,g){return this.animate(b,e,g)}});c.extend({speed:function(a,b,e){var g=a&&typeof a==="object"?a:{complete:e||!e&&b||c.isFunction(a)&&a,duration:a,easing:e&&b||b&&!c.isFunction(b)&&b};g.duration=c.fx.off?0:typeof g.duration===
-
$pxs_bg2,
+
"number"?g.duration:c.fx.speeds[g.duration]||c.fx.speeds._default;g.old=g.complete;g.complete=function(){g.queue!==false&&c(this).dequeue();c.isFunction(g.old)&&g.old.call(this)};return g},easing:{linear:function(a,b,e,g){return e+g*a},swing:function(a,b,e,g){return(-Math.cos(a*Math.PI)/2+0.5)*g+e}},timers:[],fx:function(a,b,e){this.options=b;this.elem=a;this.prop=e;if(!b.orig)b.orig={}}});c.fx.prototype={update:function(){this.options.step&&this.options.step.call(this.elem,this.now,this);(c.fx.step[this.prop]||
-
$pxs_bg1,
+
c.fx.step._default)(this);if((this.prop==="height"||this.prop==="width")&&this.elem.style)this.elem.style.display="block"},cur:function(a){if(this.elem[this.prop]!=null&&(!this.elem.style||this.elem.style[this.prop]==null))return this.elem[this.prop];return(a=parseFloat(c.css(this.elem,this.prop,a)))&&a>-10000?a:parseFloat(c.curCSS(this.elem,this.prop))||0},custom:function(a,b,e){function g(l){return f.step(l)}this.startTime=z();this.start=a;this.end=b;this.unit=e||this.unit||"px";this.now=this.start;
-
o.speed,
+
this.pos=this.state=0;var f=this;g.elem=this.elem;if(g()&&c.timers.push(g)&&!na)na=setInterval(c.fx.tick,13)},show:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.show=true;this.custom(this.prop==="width"||this.prop==="height"?1:0,this.cur());c(this.elem).show()},hide:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.hide=true;this.custom(this.cur(),0)},step:function(a){var b=z(),e=true;if(a||b>=this.options.duration+this.startTime){this.now=
-
o.easing,
+
this.end;this.pos=this.state=1;this.update();this.options.curAnim[this.prop]=true;for(var g in this.options.curAnim)if(this.options.curAnim[g]!==true)e=false;if(e){if(this.options.display!=null){this.elem.style.overflow=this.options.overflow;a=c.data(this.elem,"olddisplay");this.elem.style.display=a?a:this.options.display;if(c.css(this.elem,"display")==="none")this.elem.style.display="block"}this.options.hide&&c(this.elem).hide();if(this.options.hide||this.options.show)for(var f in this.options.curAnim)c.style(this.elem,
-
o.easingBg);
+
f,this.options.orig[f]);this.options.complete.call(this.elem)}return false}else{f=b-this.startTime;this.state=f/this.options.duration;a=this.options.easing||(c.easing.swing?"swing":"linear");this.pos=c.easing[this.options.specialEasing&&this.options.specialEasing[this.prop]||a](this.state,f,0,1,this.options.duration);this.now=this.start+(this.end-this.start)*this.pos;this.update()}return true}};c.extend(c.fx,{tick:function(){for(var a=c.timers,b=0;b<a.length;b++)a[b]()||a.splice(b--,1);a.length||
-
});
+
c.fx.stop()},stop:function(){clearInterval(na);na=null},speeds:{slow:600,fast:200,_default:400},step:{opacity:function(a){c.style(a.elem,"opacity",a.now)},_default:function(a){if(a.elem.style&&a.elem.style[a.prop]!=null)a.elem.style[a.prop]=(a.prop==="width"||a.prop==="height"?Math.max(0,a.now):a.now)+a.unit;else a.elem[a.prop]=a.now}}});if(c.expr&&c.expr.filters)c.expr.filters.animated=function(a){return c.grep(c.timers,function(b){return a===b.elem}).length};c.fn.offset="getBoundingClientRect"in
-
+
v.documentElement?function(a){var b=this[0];if(a)return this.each(function(f){c.offset.setOffset(this,a,f)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);var e=b.getBoundingClientRect(),g=b.ownerDocument;b=g.body;g=g.documentElement;return{top:e.top+(self.pageYOffset||c.support.boxModel&&g.scrollTop||b.scrollTop)-(g.clientTop||b.clientTop||0),left:e.left+(self.pageXOffset||c.support.boxModel&&g.scrollLeft||b.scrollLeft)-(g.clientLeft||b.clientLeft||
-
/*
+
0)}}:function(a){var b=this[0];if(a)return this.each(function(y){c.offset.setOffset(this,a,y)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);c.offset.initialize();var e=b.offsetParent,g=b,f=b.ownerDocument,l,k=f.documentElement,s=f.body;g=(f=f.defaultView)?f.getComputedStyle(b,null):b.currentStyle;for(var m=b.offsetTop,r=b.offsetLeft;(b=b.parentNode)&&b!==s&&b!==k;){if(c.offset.supportsFixedPosition&&g.position==="fixed")break;l=f?f.getComputedStyle(b,
-
clicking a thumb will slide to the respective image
+
null):b.currentStyle;m-=b.scrollTop;r-=b.scrollLeft;if(b===e){m+=b.offsetTop;r+=b.offsetLeft;if(c.offset.doesNotAddBorder&&!(c.offset.doesAddBorderForTableAndCells&&/^t(able|d|h)$/i.test(b.nodeName))){m+=parseFloat(l.borderTopWidth)||0;r+=parseFloat(l.borderLeftWidth)||0}g=e;e=b.offsetParent}if(c.offset.subtractsBorderForOverflowNotVisible&&l.overflow!=="visible"){m+=parseFloat(l.borderTopWidth)||0;r+=parseFloat(l.borderLeftWidth)||0}g=l}if(g.position==="relative"||g.position==="static"){m+=s.offsetTop;
-
*/
+
r+=s.offsetLeft}if(c.offset.supportsFixedPosition&&g.position==="fixed"){m+=Math.max(k.scrollTop,s.scrollTop);r+=Math.max(k.scrollLeft,s.scrollLeft)}return{top:m,left:r}};c.offset={initialize:function(){var a=v.body,b=v.createElement("div"),e,g,f,l=parseFloat(c.curCSS(a,"marginTop",true))||0;c.extend(b.style,{position:"absolute",top:0,left:0,margin:0,border:0,width:"1px",height:"1px",visibility:"hidden"});b.innerHTML="<div style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;'><div></div></div><table style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;' cellpadding='0' cellspacing='0'><tr><td></td></tr></table>";
-
$thumbs.bind('click',function(){
+
a.insertBefore(b,a.firstChild);e=b.firstChild;g=e.firstChild;f=e.nextSibling.firstChild.firstChild;this.doesNotAddBorder=g.offsetTop!==5;this.doesAddBorderForTableAndCells=f.offsetTop===5;g.style.position="fixed";g.style.top="20px";this.supportsFixedPosition=g.offsetTop===20||g.offsetTop===15;g.style.position=g.style.top="";e.style.overflow="hidden";e.style.position="relative";this.subtractsBorderForOverflowNotVisible=g.offsetTop===-5;this.doesNotIncludeMarginInBodyOffset=a.offsetTop!==l;a.removeChild(b);
-
var $thumb = $(this);
+
c.offset.initialize=c.noop},bodyOffset:function(a){var b=a.offsetTop,e=a.offsetLeft;c.offset.initialize();if(c.offset.doesNotIncludeMarginInBodyOffset){b+=parseFloat(c.curCSS(a,"marginTop",true))||0;e+=parseFloat(c.curCSS(a,"marginLeft",true))||0}return{top:b,left:e}},setOffset:function(a,b,e){if(/static/.test(c.curCSS(a,"position")))a.style.position="relative";var g=c(a),f=g.offset(),l=parseInt(c.curCSS(a,"top",true),10)||0,k=parseInt(c.curCSS(a,"left",true),10)||0;if(c.isFunction(b))b=b.call(a,
-
highlight($thumb);
+
e,f);e={top:b.top-f.top+l,left:b.left-f.left+k};"using"in b?b.using.call(a,e):g.css(e)}};c.fn.extend({position:function(){if(!this[0])return null;var a=this[0],b=this.offsetParent(),e=this.offset(),g=/^body|html$/i.test(b[0].nodeName)?{top:0,left:0}:b.offset();e.top-=parseFloat(c.curCSS(a,"marginTop",true))||0;e.left-=parseFloat(c.curCSS(a,"marginLeft",true))||0;g.top+=parseFloat(c.curCSS(b[0],"borderTopWidth",true))||0;g.left+=parseFloat(c.curCSS(b[0],"borderLeftWidth",true))||0;return{top:e.top-
-
//if autoplay interrupt when user clicks
+
g.top,left:e.left-g.left}},offsetParent:function(){return this.map(function(){for(var a=this.offsetParent||v.body;a&&!/^body|html$/i.test(a.nodeName)&&c.css(a,"position")==="static";)a=a.offsetParent;return a})}});c.each(["Left","Top"],function(a,b){var e="scroll"+b;c.fn[e]=function(g){var f=this[0],l;if(!f)return null;if(g!==j)return this.each(function(){if(l=V(this))l.scrollTo(!a?g:c(l).scrollLeft(),a?g:c(l).scrollTop());else this[e]=g});else return(l=V(f))?"pageXOffset"in l?l[a?"pageYOffset":"pageXOffset"]:
-
if(o.auto)
+
c.support.boxModel&&l.document.documentElement[e]||l.document.body[e]:f[e]}});c.each(["Height","Width"],function(a,b){var e=b.toLowerCase();c.fn["inner"+b]=function(){return this[0]?c.css(this[0],e,false,"padding"):null};c.fn["outer"+b]=function(g){return this[0]?c.css(this[0],e,false,g?"margin":"border"):null};c.fn[e]=function(g){var f=this[0];if(!f)return g==null?null:this;if(c.isFunction(g))return this.each(function(l){var k=c(this);k[e](g.call(this,l,k[e]()))});return"scrollTo"in f&&f.document?
-
clearInterval(slideshow);
+
f.document.compatMode==="CSS1Compat"&&f.document.documentElement["client"+b]||f.document.body["client"+b]:f.nodeType===9?Math.max(f.documentElement["client"+b],f.body["scroll"+b],f.documentElement["scroll"+b],f.body["offset"+b],f.documentElement["offset"+b]):g===j?c.css(f,e):this.css(e,typeof g==="string"?g:g+"px")}});p.jQuery=p.$=c})(window);jQuery.easing.jswing=jQuery.easing.swing;
-
current = $thumb.index();
+
jQuery.extend(jQuery.easing,{def:"easeOutQuad",swing:function(p,j,u,t,o){return jQuery.easing[jQuery.easing.def](p,j,u,t,o)},easeInQuad:function(p,j,u,t,o){return t*(j/=o)*j+u},easeOutQuad:function(p,j,u,t,o){return-t*(j/=o)*(j-2)+u},easeInOutQuad:function(p,j,u,t,o){if((j/=o/2)<1)return t/2*j*j+u;return-t/2*(--j*(j-2)-1)+u},easeInCubic:function(p,j,u,t,o){return t*(j/=o)*j*j+u},easeOutCubic:function(p,j,u,t,o){return t*((j=j/o-1)*j*j+1)+u},easeInOutCubic:function(p,j,u,t,o){if((j/=o/2)<1)return t/
-
slide(current,
+
2*j*j*j+u;return t/2*((j-=2)*j*j+2)+u},easeInQuart:function(p,j,u,t,o){return t*(j/=o)*j*j*j+u},easeOutQuart:function(p,j,u,t,o){return-t*((j=j/o-1)*j*j*j-1)+u},easeInOutQuart:function(p,j,u,t,o){if((j/=o/2)<1)return t/2*j*j*j*j+u;return-t/2*((j-=2)*j*j*j-2)+u},easeInQuint:function(p,j,u,t,o){return t*(j/=o)*j*j*j*j+u},easeOutQuint:function(p,j,u,t,o){return t*((j=j/o-1)*j*j*j*j+1)+u},easeInOutQuint:function(p,j,u,t,o){if((j/=o/2)<1)return t/2*j*j*j*j*j+u;return t/2*((j-=2)*j*j*j*j+2)+u},easeInSine:function(p,
-
$pxs_slider,
+
j,u,t,o){return-t*Math.cos(j/o*(Math.PI/2))+t+u},easeOutSine:function(p,j,u,t,o){return t*Math.sin(j/o*(Math.PI/2))+u},easeInOutSine:function(p,j,u,t,o){return-t/2*(Math.cos(Math.PI*j/o)-1)+u},easeInExpo:function(p,j,u,t,o){return j==0?u:t*Math.pow(2,10*(j/o-1))+u},easeOutExpo:function(p,j,u,t,o){return j==o?u+t:t*(-Math.pow(2,-10*j/o)+1)+u},easeInOutExpo:function(p,j,u,t,o){if(j==0)return u;if(j==o)return u+t;if((j/=o/2)<1)return t/2*Math.pow(2,10*(j-1))+u;return t/2*(-Math.pow(2,-10*--j)+2)+u},
-
$pxs_bg3,
+
easeInCirc:function(p,j,u,t,o){return-t*(Math.sqrt(1-(j/=o)*j)-1)+u},easeOutCirc:function(p,j,u,t,o){return t*Math.sqrt(1-(j=j/o-1)*j)+u},easeInOutCirc:function(p,j,u,t,o){if((j/=o/2)<1)return-t/2*(Math.sqrt(1-j*j)-1)+u;return t/2*(Math.sqrt(1-(j-=2)*j)+1)+u},easeInElastic:function(p,j,u,t,o){p=1.70158;var z=0,G=t;if(j==0)return u;if((j/=o)==1)return u+t;z||(z=o*0.3);if(G<Math.abs(t)){G=t;p=z/4}else p=z/(2*Math.PI)*Math.asin(t/G);return-(G*Math.pow(2,10*(j-=1))*Math.sin((j*o-p)*2*Math.PI/z))+u},easeOutElastic:function(p,
-
$pxs_bg2,
+
j,u,t,o){p=1.70158;var z=0,G=t;if(j==0)return u;if((j/=o)==1)return u+t;z||(z=o*0.3);if(G<Math.abs(t)){G=t;p=z/4}else p=z/(2*Math.PI)*Math.asin(t/G);return G*Math.pow(2,-10*j)*Math.sin((j*o-p)*2*Math.PI/z)+t+u},easeInOutElastic:function(p,j,u,t,o){p=1.70158;var z=0,G=t;if(j==0)return u;if((j/=o/2)==2)return u+t;z||(z=o*0.3*1.5);if(G<Math.abs(t)){G=t;p=z/4}else p=z/(2*Math.PI)*Math.asin(t/G);if(j<1)return-0.5*G*Math.pow(2,10*(j-=1))*Math.sin((j*o-p)*2*Math.PI/z)+u;return G*Math.pow(2,-10*(j-=1))*Math.sin((j*
-
$pxs_bg1,
+
o-p)*2*Math.PI/z)*0.5+t+u},easeInBack:function(p,j,u,t,o,z){if(z==undefined)z=1.70158;return t*(j/=o)*j*((z+1)*j-z)+u},easeOutBack:function(p,j,u,t,o,z){if(z==undefined)z=1.70158;return t*((j=j/o-1)*j*((z+1)*j+z)+1)+u},easeInOutBack:function(p,j,u,t,o,z){if(z==undefined)z=1.70158;if((j/=o/2)<1)return t/2*j*j*(((z*=1.525)+1)*j-z)+u;return t/2*((j-=2)*j*(((z*=1.525)+1)*j+z)+2)+u},easeInBounce:function(p,j,u,t,o){return t-jQuery.easing.easeOutBounce(p,o-j,0,t,o)+u},easeOutBounce:function(p,j,u,t,o){return(j/=
-
o.speed,
+
o)<1/2.75?t*7.5625*j*j+u:j<2/2.75?t*(7.5625*(j-=1.5/2.75)*j+0.75)+u:j<2.5/2.75?t*(7.5625*(j-=2.25/2.75)*j+0.9375)+u:t*(7.5625*(j-=2.625/2.75)*j+0.984375)+u},easeInOutBounce:function(p,j,u,t,o){if(j<o/2)return jQuery.easing.easeInBounce(p,j*2,0,t,o)*0.5+u;return jQuery.easing.easeOutBounce(p,j*2-o,0,t,o)*0.5+t*0.5+u}});
-
o.easing,
+
(function(p){function j(t){for(var o=["transform","WebkitTransform","MozTransform"],z;z=o.shift();)if(typeof t.style[z]!="undefined")return z;return"transform"}var u=p.fn.css;p.fn.css=function(t){if(typeof p.props.transform=="undefined"&&(t=="transform"||typeof t=="object"&&typeof t.transform!="undefined"))p.props.transform=j(this.get(0));if(t=="transform")t=p.props.transform;return u.apply(this,arguments)}})(jQuery);
-
o.easingBg);
+
(function(p){var j="deg";p.fn.rotate=function(o){var z=p(this).css("transform")||"none";if(typeof o=="undefined"){if(z)if((o=z.match(/rotate\(([^)]+)\)/))&&o[1])return o[1];return 0}if(o=o.toString().match(/^(-?\d+(\.\d+)?)(.+)?$/)){if(o[3])j=o[3];p(this).css("transform",z.replace(/none|rotate\([^)]*\)/,"")+"rotate("+o[1]+j+")")}};p.fn.scale=function(o){var z=p(this).css("transform");if(typeof o=="undefined"){if(z)if((o=z.match(/scale\(([^)]+)\)/))&&o[1])return o[1];return 1}p(this).css("transform",
-
});
+
z.replace(/none|scale\([^)]*\)/,"")+"scale("+o+")")};var u=p.fx.prototype.cur;p.fx.prototype.cur=function(){if(this.prop=="rotate")return parseFloat(p(this.elem).rotate());else if(this.prop=="scale")return parseFloat(p(this.elem).scale());return u.apply(this,arguments)};p.fx.step.rotate=function(o){p(o.elem).rotate(o.now+j)};p.fx.step.scale=function(o){p(o.elem).scale(o.now)};var t=p.fn.animate;p.fn.animate=function(o){if(typeof o.rotate!="undefined"){var z=o.rotate.toString().match(/^(([+-]=)?(-?\d+(\.\d+)?))(.+)?$/);
-
+
if(z&&z[5])j=z[5];o.rotate=z[1]}return t.apply(this,arguments)}})(jQuery);
-
+
(function(p){p.fn.quicksand=function(j,u,t){var o={duration:750,easing:"swing",attribute:"data-id",adjustHeight:"auto",useScaling:true,enhancement:function(){},selector:"> *"};p.extend(o,u);if(p.browser.msie||typeof p.fn.scale=="undefined")o.useScaling=false;var z;if(typeof u=="function")z=u;else if(typeof(t=="function"))z=t;return this.each(function(G){var U,R=[],S=p(j).clone(),L=p(this);G=p(this).css("height");var Z,ea=false,fa=p(L).offset(),T=[],V=p(this).find(o.selector);if(p.browser.msie&&p.browser.version.substr(0,
-
+
1)<7)L.html("").append(S);else{var c=0,oa=function(){if(!c){L.html(N.html());typeof z=="function"&&z.call(this);ea&&L.css("height",Z);o.enhancement(L);c=1}},J=L.offsetParent(),v=J.offset();if(J.css("position")=="relative"){if(J.get(0).nodeName.toLowerCase()!="body"){v.top+=parseFloat(J.css("border-top-width"));v.left+=parseFloat(J.css("border-left-width"))}}else{v.top-=parseFloat(J.css("border-top-width"));v.left-=parseFloat(J.css("border-left-width"));v.top-=parseFloat(J.css("margin-top"));v.left-=
-
/*
+
parseFloat(J.css("margin-left"))}L.css("height",p(this).height());V.each(function(K){T[K]=p(this).offset()});p(this).stop();V.each(function(K){p(this).stop();var M=p(this).get(0);M.style.position="absolute";M.style.margin="0";M.style.top=T[K].top-parseFloat(M.style.marginTop)-v.top+"px";M.style.left=T[K].left-parseFloat(M.style.marginLeft)-v.left+"px"});var N=p(L).clone();J=N.get(0);J.innerHTML="";J.setAttribute("id","");J.style.height="auto";J.style.width=L.width()+"px";N.append(S);N.insertBefore(L);
-
activate the autoplay mode if
+
N.css("opacity",0);J.style.zIndex=-1;J.style.margin="0";J.style.position="absolute";J.style.top=fa.top-v.top+"px";J.style.left=fa.left-v.left+"px";if(o.adjustHeight==="dynamic")L.animate({height:N.height()},o.duration,o.easing);else if(o.adjustHeight==="auto"){Z=N.height();if(parseFloat(G)<parseFloat(Z))L.css("height",Z);else ea=true}V.each(function(){var K=[];if(typeof o.attribute=="function"){U=o.attribute(p(this));S.each(function(){if(o.attribute(this)==U){K=p(this);return false}})}else K=S.filter("["+
-
that option was specified
+
o.attribute+"="+p(this).attr(o.attribute)+"]");if(K.length)o.useScaling?R.push({element:p(this),animation:{top:K.offset().top-v.top,left:K.offset().left-v.left,opacity:1,scale:"1.0"}}):R.push({element:p(this),animation:{top:K.offset().top-v.top,left:K.offset().left-v.left,opacity:1}});else o.useScaling?R.push({element:p(this),animation:{opacity:"0.0",scale:"0.0"}}):R.push({element:p(this),animation:{opacity:"0.0"}})});S.each(function(){var K=[],M=[];if(typeof o.attribute=="function"){U=o.attribute(p(this));
-
*/
+
V.each(function(){if(o.attribute(this)==U){K=p(this);return false}});S.each(function(){if(o.attribute(this)==U){M=p(this);return false}})}else{K=V.filter("["+o.attribute+"="+p(this).attr(o.attribute)+"]");M=S.filter("["+o.attribute+"="+p(this).attr(o.attribute)+"]")}var ka;if(K.length===0){ka=o.useScaling?{opacity:"1.0",scale:"1.0"}:{opacity:"1.0"};d=M.clone();var $=d.get(0);$.style.position="absolute";$.style.margin="0";$.style.top=M.offset().top-v.top+"px";$.style.left=M.offset().left-v.left+"px";
-
if(o.auto != 0){
+
d.css("opacity",0);o.useScaling&&d.css("transform","scale(0.0)");d.appendTo(L);R.push({element:p(d),animation:ka})}});N.remove();o.enhancement(L);for(G=0;G<R.length;G++)R[G].element.animate(R[G].animation,o.duration,o.easing,oa)}})}})(jQuery);
-
o.circular = true;
+
-
slideshow = setInterval(function(){
+
-
$pxs_next.trigger('click');
+
-
},o.auto);
+
-
}
+
-
+
-
/*
+
-
when resizing the window,
+
-
we need to recalculate the widths of the
+
-
slider elements, based on the new windows width.
+
-
we need to slide again to the current one,
+
-
since the left of the slider is no longer correct
+
-
*/
+
-
$(window).resize(function(){
+
-
w_w = $(window).width();
+
-
setWidths($pxs_slider,$elems,total_elems,$pxs_bg1,$pxs_bg2,$pxs_bg3,one_image_w,$pxs_next,$pxs_prev);
+
-
slide(current,
+
-
$pxs_slider,
+
-
$pxs_bg3,
+
-
$pxs_bg2,
+
-
$pxs_bg1,
+
-
1,
+
-
o.easing,
+
-
o.easingBg);
+
-
});
+
-
}
+
</script>
-
}).error(function(){
+
<script type="text/javascript">
-
alert('here')
+
(function(A){
-
}).attr('src',$img.attr('src'));
+
A.fn.th_video_focus=function(E){
-
});
+
-
+
var G={
-
+
actClass:"cur",
-
+
navContainerClass:".focus_pic_preview",
-
});
+
focusContainerClass:".focus_pic",
-
};
+
animTime:600,
 +
delayTime:5000
 +
};
 +
 +
if(E){
 +
A.extend(G,E)
 +
}
 +
 +
var C=G.actClass, D=G.navContainerClass, B=G.focusContainerClass, F=G.animTime, H=G.delayTime, I=null;
 +
 +
return this.each(function(){
 +
 +
var O=A(this), M=A(D+" li",O), P=A(B+" li",O), L=M.length, K=O.height();
 +
 +
function N(R){
 +
var V=K*R*-1;
 +
var U=A(B+" li",O), W=null, T=null;
 +
for(var S=0;S<=R;S++){
 +
W=U.eq(S);
 +
T=W.find('script[type="text/templ"]');
 +
if(T.length>0){
 +
W.html(T.html())
 +
}
 +
}
 +
A(B,O).stop().animate({top:V},F,function(){
 +
var Y=O.find("h3"), X=Y.height();
 +
Y.height(0).html(A(B+" li").eq(R).find("img").attr("alt")).animate({height:X},600)
 +
});
 +
A(D+" li").eq(R).addClass(C).siblings().removeClass(C)
 +
}
 +
 +
function Q(){
 +
if(I){
 +
clearInterval(I)
 +
}
 +
I=setInterval(function(){
 +
var R=A(D+" li."+C).index();
 +
N((R+1)%L)
 +
},H)
 +
}
 +
 +
O.hover(function(){
 +
if(I){
 +
clearInterval(I)
 +
}
 +
},function(){
 +
Q()
 +
});
 +
 +
var J=null;
 +
 +
M.hover(function(){
-
//the current windows width
+
var R=A(this).index();
-
var w_w = $(window).width();
+
-
var slide = function(current,
+
if(I){
-
$pxs_slider,
+
clearInterval(I)
-
$pxs_bg3,
+
-
$pxs_bg2,
+
-
$pxs_bg1,
+
-
speed,
+
-
easing,
+
-
easingBg){
+
-
var slide_to = parseInt(-w_w * current);
+
-
$pxs_slider.stop().animate({
+
-
left : slide_to + 'px'
+
-
},speed, easing);
+
-
$pxs_bg3.stop().animate({
+
-
left : slide_to/2 + 'px'
+
-
},speed, easingBg);
+
-
$pxs_bg2.stop().animate({
+
-
left : slide_to/4 + 'px'
+
-
},speed, easingBg);
+
-
$pxs_bg1.stop().animate({
+
-
left : slide_to/8 + 'px'
+
-
},speed, easingBg);
+
}
}
-
+
J=setTimeout(function(){
-
var highlight = function($elem){
+
N(R)
-
$elem.siblings().removeClass('selected');
+
},300)
-
$elem.addClass('selected');
+
},function(){
 +
if(J){
 +
clearTimeout(J)
}
}
-
+
Q()
-
var setWidths = function($pxs_slider,
+
}).click(function(T){
-
$elems,
+
var R=A(this).index(), S=P.eq(R).find("a");
-
total_elems,
+
if(document.uniqueID||window.opera){
-
$pxs_bg1,
+
S[0].click();
-
$pxs_bg2,
+
T.stopPropagation();
-
$pxs_bg3,
+
T.preventDefault()
-
one_image_w,
+
-
$pxs_next,
+
-
$pxs_prev){
+
-
/*
+
-
the width of the slider is the windows width
+
-
times the total number of elements in the slider
+
-
*/
+
-
var pxs_slider_w = w_w * total_elems;
+
-
$pxs_slider.width(pxs_slider_w + 'px');
+
-
//each element will have a width = windows width
+
-
$elems.width(w_w + 'px');
+
-
/*
+
-
we also set the width of each bg image div.
+
-
The value is the same calculated for the pxs_slider
+
-
*/
+
-
$pxs_bg1.width(pxs_slider_w + 'px');
+
-
$pxs_bg2.width(pxs_slider_w + 'px');
+
-
$pxs_bg3.width(pxs_slider_w + 'px');
+
-
+
-
/*
+
-
both the right and left of the
+
-
navigation next and previous buttons will be:
+
-
windowWidth/2 - imgWidth/2 + some margin (not to touch the image borders)
+
-
*/
+
-
var position_nav = w_w/2 - one_image_w/2 + 3;
+
-
$pxs_next.css('right', position_nav + 'px');
+
-
$pxs_prev.css('left', position_nav + 'px');
+
}
}
-
 
-
$.fn.parallaxSlider.defaults = {
 
-
auto : 0, //how many seconds to periodically slide the content.
 
-
//If set to 0 then autoplay is turned off.
 
-
speed : 1000,//speed of each slide animation
 
-
easing : 'jswing',//easing effect for the slide animation
 
-
easingBg : 'jswing',//easing effect for the background animation
 
-
circular : true,//circular slider
 
-
thumbRotation : true//the thumbs will be randomly rotated
 
-
};
 
-
//easeInOutExpo,easeInBack
 
-
})(jQuery);
 
-
</script>
 
-
 
-
<script type="text/javascript">
 
-
$(function() {
 
-
var $pxs_container = $('#pxs_container');
 
-
$pxs_container.parallaxSlider();
 
});
});
-
        </script>
 
-
<script type="text/javascript">
 
-
            $(function() {
 
-
                var d=300;
 
-
                $('#navigation a').each(function(){
 
-
                    $(this).stop().animate({
 
-
                        'marginTop':'-80px'
 
-
                    },d+=150);
 
-
                });
 
-
 
-
                $('#navigation > li').hover(
 
-
                function () {
 
-
                    $('a',$(this)).stop().animate({
 
-
                        'marginTop':'-2px'
 
-
                    },200);
 
-
                },
 
-
                function () {
 
-
                    $('a',$(this)).stop().animate({
 
-
                        'marginTop':'-80px'
 
-
                    },200);
 
-
                }
 
-
            );
 
-
            });
 
-
/*
+
Q()
-
function $2(element){
+
-
return element = document.getElementById(element);
+
})
-
}
+
-
function $D(){
+
-
var d=$2('class1content');
+
-
var h=d.offsetHeight;
+
-
var maxh=300;
+
-
function dmove(){
+
-
h+=50; //设置层展开的速度23sc.cn
+
-
if(h>=maxh){
+
-
d.style.height='300px';
+
-
clearInterval(iIntervalId);
+
-
}else{
+
-
d.style.display='block';
+
-
d.style.height=h+'px';
+
-
}
+
-
}
+
-
iIntervalId=setInterval(dmove,2);
+
-
}
+
-
function $D2(){
+
-
var d=$2('class1content');
+
-
var h=d.offsetHeight;
+
-
var maxh=300;
+
-
function dmove(){
+
-
h-=50;//设置层收缩的速度23sc.cn
+
-
if(h<=0){
+
-
d.style.display='none';
+
-
clearInterval(iIntervalId);
+
-
}else{
+
-
d.style.height=h+'px';
+
-
}
+
-
}
+
-
iIntervalId=setInterval(dmove,2);
+
-
}
+
-
function $use(){
+
-
var d=$2('class1content');
+
-
var sb=$2('stateBut');
+
-
if(d.style.display=='none'){
+
-
$D();
+
-
sb.innerHTML='unfold';
+
-
}else{
+
-
$D2();
+
-
sb.innerHTML='shrink';
+
-
}
+
-
}*/
+
-
 
+
-
 
+
-
 
+
-
function $aa(obj)
+
-
{
+
-
if(obj.className == "aa")
+
-
{
+
-
obj.className = "bb";
+
-
}
+
-
else
+
-
obj.className = "aa";
+
-
}
+
-
function unfold(element){
+
-
var temp=document.getElementById(element);
+
-
$aa(temp);
+
}
}
-
        </script>
+
 +
})(jQuery);
-
</body>
+
$(function(){
 +
 
 +
$(".film_focus").th_video_focus({
 +
navContainerClass:".film_focus_nav",
 +
focusContainerClass:".film_focus_imgs",
 +
delayTime:3000
 +
});
 +
 +
});
 +
</script>
</html>
</html>

Latest revision as of 03:32, 28 September 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd">

NJU_NJUT_IGEM

  • TEAM PHOTO
  • CRISPR/Cas
  • PCR product
  • Doing the experiment

photo introduction

  •             TEAM

  •             CRISPR

  •             PCR

  •             experiment



    All cellular systems evolve ways to combat predators and genomic parasites. In bacteria and archaea, numerous resistance mechanisms have been developed against phage. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) adaptive immune systems are found in bacteria and archaea to protect the hosts against the invasion of viruses and plasmids. Most of the bacteria and archaea acquire virus resistance by integrating short viral nucleotide acid fragments into the CRISPR sequences.....

Read More

Combing the outstanding students from Nanjing University and Nanjing University of Technology, we are trying to make the CRISPR/Cas systems work in the eukaryote cell with the help of the synthetic biology methods. Furthermore, considering the spacers’ sequence as the result of evolution, our team is developing a highly-probable algorithm to direct the synthesis and seeking of spacers or proto-spacers.

  • support

    Thanks to Dr.Xingxu Huang for his kind support in supplying us the cas9 gene.

  • Thanks to KeyGene Biotech for its help in providing technical support.

  •  Thanks to Fundamental Biological Experiment Education Center（南大生科教学实验中心） for its support in providing the equipment.

  • Attributions:

    Design

    Ruitao Jin Min Xi

    Modeling:

    Ruitao Jin Min Xi NJUT

    Wiki:

    NJUT Ning Meng Xiali Luan Jie Tang Sitong He

    Experiment:

    Lin Lei YunYun He Zhe Cao Tian Li

 

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Retrieved from "http://2013.igem.org/Team:NJU_NJUT_China"