Template:Team:SydneyUni Australia/Calendar/Events List

From 2013.igem.org

(Difference between revisions)
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},
},
// -----------------Week 3 ----------------
// -----------------Week 3 ----------------
 +
{
 +
title: 'Meeting with Yagiz',
 +
start: new Date(2013, 5, 2),
 +
description: '<b>Members:</b> Rob <br> <b>What we did: </b> WMet with Yagiz at Macquarie University to talk about Strange Nature and possible collaboration with our iGEM teams.'
 +
},
{
{
title: 'PCR analysis and GC analysis of DCA metabolism',
title: 'PCR analysis and GC analysis of DCA metabolism',
-
start: new Date(2013, 4, 3),
+
start: new Date(2013, 5, 3),
description: '<b>Members:</b> Coleman, Hugh, Rob <br> <b>What we did: </b> We ran a gel of last Friday’s PCR. The PCR was of pBS(ToMO) extracted from Wood’s E. Coli TG1, with primers iGEM 1 & 2. The banding on the gel was obscure, potentially due to too much template and could not be used. <br><br> We used the Gas Chromatographer (GC) to look at our two DCA treatments of E. Coli (transformed with either pBBR or pBS(ToMO)). Both had pretty much the same reading but it is uncertain yet whether this is because pBS(ToMO) was lost or altered during the extended incubation from Week 2, or whether ToMO was unable to degrade DCA. We also set up standardised solutions of NaCl in KP buffer for a future Cl assay.'
description: '<b>Members:</b> Coleman, Hugh, Rob <br> <b>What we did: </b> We ran a gel of last Friday’s PCR. The PCR was of pBS(ToMO) extracted from Wood’s E. Coli TG1, with primers iGEM 1 & 2. The banding on the gel was obscure, potentially due to too much template and could not be used. <br><br> We used the Gas Chromatographer (GC) to look at our two DCA treatments of E. Coli (transformed with either pBBR or pBS(ToMO)). Both had pretty much the same reading but it is uncertain yet whether this is because pBS(ToMO) was lost or altered during the extended incubation from Week 2, or whether ToMO was unable to degrade DCA. We also set up standardised solutions of NaCl in KP buffer for a future Cl assay.'
},
},
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},
},
// -----------------Week 5 ----------------
// -----------------Week 5 ----------------
 +
{
 +
title: 'Meeting Mac Uni Team',
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start: new Date(2013, 6, 1),
 +
description: '<b>Members:</b> Rob, Andrew, Viv, Shuravi, Desmond, Cyril<br> <b>What we did: </b> Lasertag and bowling with the Macquarie University iGEM team.  '
 +
},
{
{
title: 'ToMO Chloride Assay',
title: 'ToMO Chloride Assay',
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end: new Date(2013, 6, 12),
end: new Date(2013, 6, 12),
description: '<b>Members:</b> Elissa, Cyril, Desmond, Rob<br> <b>What we did: </b> We tried to PCR the origin of replication from pBBR1-MCS2 using primer iGEM 5 and 6. We digested the plasmid with EcoR1 ran a diagnostic PCR but didn’t see anything. We re-digested the plasmid with a higher concentration of DNA, and did a gradient PCR, and didn’t see the desired PCR product. We’re looking into mispriming elsewhere on pBBR. '
description: '<b>Members:</b> Elissa, Cyril, Desmond, Rob<br> <b>What we did: </b> We tried to PCR the origin of replication from pBBR1-MCS2 using primer iGEM 5 and 6. We digested the plasmid with EcoR1 ran a diagnostic PCR but didn’t see anything. We re-digested the plasmid with a higher concentration of DNA, and did a gradient PCR, and didn’t see the desired PCR product. We’re looking into mispriming elsewhere on pBBR. '
 +
},
 +
{
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title: 'Gibson Planning',
 +
start: new Date(2013, 6, 12),
 +
description: '<b>Members:</b> Nick, Rob, Andrew, Viv, Shuravi, Desmond, Evelyn<br> <b>What we did: </b> Met to dicuss Gibson Assembly and decided to pursue this method of assembly. Delegated some tasks for finding sequences, optimisation and ordering of gBlocks from IDT using the special iGEM discount. '
},
},
// -----------------Week 7 ----------------
// -----------------Week 7 ----------------

Revision as of 03:38, 28 September 2013

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