Template:Kyoto/Notebook/Aug 15
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==Aug 15== | ==Aug 15== | ||
+ | ===Liquid Culture=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !Sample||medium | ||
+ | |- | ||
+ | |8/10 BBa_B0034(1)||LB (+Amp) | ||
+ | |- | ||
+ | |8/10 BBa_B0034(2)||LB (+Amp) | ||
+ | |- | ||
+ | |8/10 BBa_R0011(1)||LB (+CP) | ||
+ | |- | ||
+ | |8/10 BBa_R0011(2)||LB (+CP) | ||
+ | |- | ||
+ | |8/10 BBa_P0412(1)||LB (+CP) | ||
+ | |- | ||
+ | |8/10 BBa_P0412(2)||LB (+CP) | ||
+ | |} | ||
+ | </div> | ||
+ | |||
+ | ===Transformation=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">Nakamoto</span> | ||
+ | {| class="wikitable" | ||
+ | !Name||Sample||Competent Cells||Total||Plate | ||
+ | |- | ||
+ | |BBa_I0500(Pbad/araC)||2||17||19||LB (+Kan) | ||
+ | |- | ||
+ | |BBa_F1610(RBS-luxI-DT)||2||17||19||LB (+Amp) | ||
+ | |- | ||
+ | |BBa_R0061(Plux)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_I13504(RBS-GFP-DT)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_I0462(RBS-luxR-DT)||2||17||19||LB (+Amp) | ||
+ | |- | ||
+ | |BBa_K117000(lysis1)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_K112806(lysis2)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_K748002(lysis3)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_B0015(DT)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_Q04400(RBS-tetR-DT-Ptet)||2||17||19||LB (+Kan) | ||
+ | |- | ||
+ | |BBa_I732020(RBS-lacZα-DT)||2||17||19||LB (+CP) | ||
+ | |- | ||
+ | |BBa_J23100||2||17||19||LB (+Amp) | ||
+ | |} | ||
+ | </div> | ||
+ | |||
+ | ===Miniprep=== | ||
+ | <div class="experiment"> | ||
+ | <span class="author">No name</span> | ||
+ | {|class="wikitable" | ||
+ | !DNA||concentration[µg/mL]||260/280||260/230 | ||
+ | |- | ||
+ | |BBa_B0034(1)||84.1||2.11||2.01 | ||
+ | |- | ||
+ | |BBa_B0034(2)||86.9||2.03||2.02 | ||
+ | |- | ||
+ | |BBa_R0011(1)||78.3||2.26||2.02 | ||
+ | |- | ||
+ | |BBa_R0011(2)||88.9||2.24||2.02 | ||
+ | |- | ||
+ | |BBa_P0412(1)||15.61||2.10||1.93 | ||
+ | |- | ||
+ | |BBa_P0412(2)||12.93||2.18||1.91 | ||
+ | |} | ||
+ | </div> | ||
+ | <!-- ここまでをコピーしてね --> |
Latest revision as of 03:42, 28 September 2013
Contents |
Aug 15
Liquid Culture
Sample | medium |
---|---|
8/10 BBa_B0034(1) | LB (+Amp) |
8/10 BBa_B0034(2) | LB (+Amp) |
8/10 BBa_R0011(1) | LB (+CP) |
8/10 BBa_R0011(2) | LB (+CP) |
8/10 BBa_P0412(1) | LB (+CP) |
8/10 BBa_P0412(2) | LB (+CP) |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
BBa_I0500(Pbad/araC) | 2 | 17 | 19 | LB (+Kan) |
BBa_F1610(RBS-luxI-DT) | 2 | 17 | 19 | LB (+Amp) |
BBa_R0061(Plux) | 2 | 17 | 19 | LB (+CP) |
BBa_I13504(RBS-GFP-DT) | 2 | 17 | 19 | LB (+CP) |
BBa_I0462(RBS-luxR-DT) | 2 | 17 | 19 | LB (+Amp) |
BBa_K117000(lysis1) | 2 | 17 | 19 | LB (+CP) |
BBa_K112806(lysis2) | 2 | 17 | 19 | LB (+CP) |
BBa_K748002(lysis3) | 2 | 17 | 19 | LB (+CP) |
BBa_B0015(DT) | 2 | 17 | 19 | LB (+CP) |
BBa_Q04400(RBS-tetR-DT-Ptet) | 2 | 17 | 19 | LB (+Kan) |
BBa_I732020(RBS-lacZα-DT) | 2 | 17 | 19 | LB (+CP) |
BBa_J23100 | 2 | 17 | 19 | LB (+Amp) |
Miniprep
DNA | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
BBa_B0034(1) | 84.1 | 2.11 | 2.01 |
BBa_B0034(2) | 86.9 | 2.03 | 2.02 |
BBa_R0011(1) | 78.3 | 2.26 | 2.02 |
BBa_R0011(2) | 88.9 | 2.24 | 2.02 |
BBa_P0412(1) | 15.61 | 2.10 | 1.93 |
BBa_P0412(2) | 12.93 | 2.18 | 1.91 |