Template:Kyoto/Notebook/Aug 15

From 2013.igem.org

(Difference between revisions)
(→Aug 15)
(→Miniprep)
 
(4 intermediate revisions not shown)
Line 1: Line 1:
==Aug 15==
==Aug 15==
===Liquid Culture===
===Liquid Culture===
-
<!-- ここから -->
 
<div class="experiment">
<div class="experiment">
-
<span class="author">No name</span>
+
<span class="author">Nakamoto</span>
{| class="wikitable"
{| class="wikitable"
!Sample||medium
!Sample||medium
|-
|-
-
|8/10 BBa_B0034(1)|RBS|  
+
|8/10 BBa_B0034(1)||LB (+Amp)
|-
|-
-
|8/10 BBa_B0034(2) |RBS|　
+
|8/10 BBa_B0034(2)||LB (+Amp)
|-
|-
-
|8/10 BBa_R0011(1)|Plac|　
+
|8/10 BBa_R0011(1)||LB (+CP)
|-
|-
-
|8/10 BBa_R0011(2)|Plac|　
+
|8/10 BBa_R0011(2)||LB (+CP)
|-
|-
-
|8/10 BBa_P0412(1) |LacI|　
+
|8/10 BBa_P0412(1)||LB (+CP)
|-
|-
-
|8/10 BBa_P0412(2) |LacI|　
+
|8/10 BBa_P0412(2)||LB (+CP)
|}
|}
</div>
</div>
-
<!-- ここまでをコピーしてね -->
+
 
-
===LB Medium Plate===
+
===Transformation===
<div class="experiment">
<div class="experiment">
<span class="author">Nakamoto</span>
<span class="author">Nakamoto</span>
{| class="wikitable"
{| class="wikitable"
-
!volume||200ml
+
!Name||Sample||Competent Cells||Total||Plate
|-
|-
-
|Bacto T2ypton || 2g
+
|BBa_I0500(Pbad/araC)||2||17||19||LB (+Kan)
|-
|-
-
|Bacto yeast extract ||1g
+
|BBa_F1610(RBS-luxI-DT)||2||17||19||LB (+Amp)
|-
|-
-
|NaCl||1g
+
|BBa_R0061(Plux)||2||17||19||LB (+CP)
|-
|-
-
|0.4&micro; NaOHaq||50&micro;L
+
|BBa_I13504(RBS-GFP-DT)||2||17||19||LB (+CP)
|-
|-
-
|Agar Pouder||2g　
+
|BBa_I0462(RBS-luxR-DT)||2||17||19||LB (+Amp)
 +
|-
 +
|BBa_K117000(lysis1)||2||17||19||LB (+CP)
 +
|-
 +
|BBa_K112806(lysis2)||2||17||19||LB (+CP)
 +
|-
 +
|BBa_K748002(lysis3)||2||17||19||LB (+CP)
 +
|-
 +
|BBa_B0015(DT)||2||17||19||LB (+CP)
 +
|-
 +
|BBa_Q04400(RBS-tetR-DT-Ptet)||2||17||19||LB (+Kan)
 +
|-
 +
|BBa_I732020(RBS-lacZ&alpha;-DT)||2||17||19||LB (+CP)
 +
|-
 +
|BBa_J23100||2||17||19||LB (+Amp)
|}
|}
</div>
</div>
 +
 +
===Miniprep===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{|class="wikitable"
 +
!DNA||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|BBa_B0034(1)||84.1||2.11||2.01
 +
|-
 +
|BBa_B0034(2)||86.9||2.03||2.02
 +
|-
 +
|BBa_R0011(1)||78.3||2.26||2.02
 +
|-
 +
|BBa_R0011(2)||88.9||2.24||2.02
 +
|-
 +
|BBa_P0412(1)||15.61||2.10||1.93
 +
|-
 +
|BBa_P0412(2)||12.93||2.18||1.91
 +
|}
 +
</div>
 +
<!-- ここまでをコピーしてね -->

Latest revision as of 03:42, 28 September 2013

Contents

Aug 15

Liquid Culture

Nakamoto

Samplemedium
8/10 BBa_B0034(1)LB (+Amp)
8/10 BBa_B0034(2)LB (+Amp)
8/10 BBa_R0011(1)LB (+CP)
8/10 BBa_R0011(2)LB (+CP)
8/10 BBa_P0412(1)LB (+CP)
8/10 BBa_P0412(2)LB (+CP)

Transformation

Nakamoto

NameSampleCompetent CellsTotalPlate
BBa_I0500(Pbad/araC)21719LB (+Kan)
BBa_F1610(RBS-luxI-DT)21719LB (+Amp)
BBa_R0061(Plux)21719LB (+CP)
BBa_I13504(RBS-GFP-DT)21719LB (+CP)
BBa_I0462(RBS-luxR-DT)21719LB (+Amp)
BBa_K117000(lysis1)21719LB (+CP)
BBa_K112806(lysis2)21719LB (+CP)
BBa_K748002(lysis3)21719LB (+CP)
BBa_B0015(DT)21719LB (+CP)
BBa_Q04400(RBS-tetR-DT-Ptet)21719LB (+Kan)
BBa_I732020(RBS-lacZα-DT)21719LB (+CP)
BBa_J2310021719LB (+Amp)

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
BBa_B0034(1)84.12.112.01
BBa_B0034(2)86.92.032.02
BBa_R0011(1)78.32.262.02
BBa_R0011(2)88.92.242.02
BBa_P0412(1)15.612.101.93
BBa_P0412(2)12.932.181.91
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Aug_15"