Team:Penn/Project

From 2013.igem.org

(Difference between revisions)
 
(93 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
+
<html lang="en">
-
  <head>
+
<head>
-
     <title></title>
+
     <title>modeling</title>
-
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
+
      <link href="https://googledrive.com/host/0B4ZBZOYYKBzEVHRaZEdUVGo5cjA" type="text/css" rel="stylesheet"/>
-
    <!-- Bootstrap and mainstyle css -->
+
    <script src="https://googledrive.com/host/0B4ZBZOYYKBzETkFqdnhMeV9fMzA" ></script>
-
    <link href="https://googledrive.com/host/0B4ZBZOYYKBzEeUFaR1lNWFZWMnc" rel="stylesheet" media="screen">
+
     <script src="https://googledrive.com/host/0B4ZBZOYYKBzEZTdBSFdUV19LYjQ" type="text/javascript"></script>
-
    <link href="https://googledrive.com/host/0B4ZBZOYYKBzEYUtsRS1ZWHM2bTQ" type="text/css" rel="stylesheet"/>
+
<script src="https://googledrive.com/host/0B4ZBZOYYKBzEblVWdXkta245Y0k/" type="text/javascript"> </script> <!--javascript-->
-
  <script src="https://googledrive.com/host/0B4ZBZOYYKBzETkFqdnhMeV9fMzA" ></script>
+
<script>
-
     <script src="https://googledrive.com/host/0B4ZBZOYYKBzEZTdBSFdUV19LYjQ"></script>
+
        $(document).ready(function($) {
-
    <script src="https://googledrive.com/host/0B4ZBZOYYKBzEOFVQV0ZLWEZqN2M"></script>
+
-
   
+
-
    <script>
+
-
        jQuery(document).ready(function($) {
+
-
       
+
-
        $('body').scrollspy({ target: '#nav-circles' });
+
-
       
+
/*load in the sidebar*/
-
        });
+
$('.left_wrap').load('https://googledrive.com/host/0B4ZBZOYYKBzEclFHMmpZcVlydmc');
-
    </script>
+
-
    <style>
+
-
        /*hero unit positioning*/
+
-
        #text {
+
-
            margin-left: 20px;
+
-
            padding: 50px;
+
-
           
+
-
        }
+
-
       
+
-
        /*navigation circles*/
+
-
        #nav-circles {
+
-
            position: fixed;
+
-
            top: 100px;
+
-
            right: 20px;
+
-
            z-index: 9999;
+
-
        }
+
-
       
+
-
        #nav-circles li {
+
-
            display: block;
+
-
            height: 30px;
+
-
            width: 30px;
+
-
            border-radius: 100%;
+
-
            margin:10px;
+
-
 
+
-
            top: 100px;
+
-
            right: 100px;
+
-
            background-color: gray;
+
-
        }
+
-
       
+
-
        /*Sections*/
+
-
        .section {
+
-
            min-height: 700px;
+
-
            min-width: 500px;
+
-
        }
+
-
       
+
-
        /*rows with images*/
+
-
        .row{
+
-
            width: 100%;
+
-
            margin-top: 100px;
+
-
        }
+
-
       
+
-
      .span4 {
+
-
            height: 150px;
+
-
            width: 150px;
+
-
            position: relative;
+
-
              -moz-border-radius: 100%;
+
-
    -webkit-border-radius: 100%;
+
-
    -khtml-border-radius: 100%;
+
-
            border-radius: 100%;
+
-
            overflow: hidden;
+
-
            background-size: 300px;
+
-
            background-position: -70px -20px;
+
-
            opacity: .7;
+
-
            margin: 50px;
+
-
            display: inline-block;
+
-
           
+
-
        }
+
-
       
+
-
        .span4 img {
+
-
            height: 150px;
+
-
         
+
-
            position: relative;
+
-
        }
+
-
       
+
-
                .span4:hover {
+
-
            opacity: 1;
+
-
        }
+
-
       
+
-
        .img-span {
+
-
    overflow: hidden;
+
-
    height: 100px;
+
-
width: 100%;
+
-
background-size: 100%;
+
-
    background-image: url("https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/uN-wv6h07i/Unified%20Artwork%20Styles%20Here/science%21%21%21/20130513135201-0.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg");
+
-
    background-attachment: fixed;
+
-
    background-repeat: no-repeat;
+
-
margin-left: 0px;
+
-
}
+
-
.hero-unit{
+
          });
-
    margin-top:10px;
+
     </script>  
-
    width: 400px;
+
<style>
-
}
+
           
-
   
+
-
          /*jumbotron colors*/
+
-
      .jumbotron {
+
-
  position: relative;
+
-
  min-height: 600px;
+
-
  padding: 40px 0;
+
-
  color: #fff;
+
-
  text-align: center;
+
-
  text-shadow: 0 1px 3px rgba(0,0,0,.4), 0 0 30px rgba(0,0,0,.075);
+
-
  background: #380060; /* Old browsers */
+
-
  background: -moz-linear-gradient(45deg,  #380060 0%, #3fffe6 100%); /* FF3.6+ */
+
-
  background: -webkit-gradient(linear, left bottom, right top, color-stop(0%,#094b42), color-stop(100%,#3fffe6)); /* Chrome,Safari4+ */
+
-
  background: -webkit-linear-gradient(45deg,  #380060 0%,#3fffe6 100%); /* Chrome10+,Safari5.1+ */
+
-
  background: -o-linear-gradient(45deg,  #380060 0%,#3fffe6 100%); /* Opera 11.10+ */
+
-
  background: -ms-linear-gradient(45deg,  #380060 0%,#3fffe6 100%); /* IE10+ */
+
-
  background: linear-gradient(45deg,  #380060 0%,#3fffe6 100%); /* W3C */
+
-
  filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#380060', endColorstr='#3fffe6',GradientType=1 ); /* IE6-9 fallback on horizontal gradient */
+
-
  -webkit-box-shadow: inset 0 3px 7px rgba(0,0,0,.2), inset 0 -3px 7px rgba(0,0,0,.2);
+
-
    -moz-box-shadow: inset 0 3px 7px rgba(0,0,0,.2), inset 0 -3px 7px rgba(0,0,0,.2);
+
-
          box-shadow: inset 0 3px 7px rgba(0,0,0,.2), inset 0 -3px 7px rgba(0,0,0,.2);
+
-
}
+
-
     
+
-
     </style>
+
-
  </head>
+
-
 
+
-
    <body data-spy="scroll" data-target="#nav-circles">
+
-
      <div class="navbar-wrapper">
+
-
      <!-- Wrap the .navbar in .container to center it within the absolutely positioned parent. -->
+
-
      <div class="container">
+
-
 
+
-
        <div class="navbar navbar-inverse navbar-fixed-top">
+
-
          <div class="navbar-inner" style="padding-left: 50px;">
+
-
            <!-- Responsive Navbar Part 1: Button for triggering responsive navbar (not covered in tutorial). Include responsive CSS to utilize. -->
+
-
            <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
+
-
              <span class="icon-bar"></span>
+
-
              <span class="icon-bar"></span>
+
-
              <span class="icon-bar"></span>
+
-
            </button>
+
-
            <a class="brand" href="https://2013.igem.org/Team:Penn">Penn's iGEM Team</a>
+
-
            <!-- Responsive Navbar Part 2: Place all navbar contents you want collapsed withing .navbar-collapse.collapse. -->
+
-
            <div class="nav-collapse collapse">
+
-
              <ul class="nav">
+
-
             
+
-
                <!-- Read about Bootstrap dropdowns at http://twitter.github.com/bootstrap/javascript.html#dropdowns -->
+
-
                <li class="dropdown">
+
-
                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">About <b class="caret"></b></a>
+
-
                  <ul class="dropdown-menu">
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Team">Team</a></li>
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Sponsors">Sponsors</a></li>
+
-
                    <li><a href="https://igem.org/Team.cgi?id=1128">Official Team Profile</a></li>
+
-
                    <!--<li class="divider"></li>
+
-
                    <li class="nav-header">Nav header</li>
+
-
                    <li><a href="#">Separated link</a></li>
+
-
                    <li><a href="#">One more separated link</a></li>-->
+
-
                  </ul>
+
-
                </li>
+
-
                <li><a href="https://2013.igem.org/Team:Penn/HumanPractices">Human Practices</a></li>
+
-
<li><a href="https://2013.igem.org/Team:Penn/Notebook">Notebook</a></li>
+
-
                <li class="dropdown">
+
-
                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
+
-
                  <ul class="dropdown-menu">
+
-
                 
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Project">Project</a></li>
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Parts">Parts</a></li>
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Modeling">Modeling</a></li>
+
-
                    <li><a href="https://2013.igem.org/Team:Penn/Safety">Safety</a></li>
+
-
                  </ul>
+
-
                </li>
+
-
              </ul>
+
-
            </div><!--/.nav-collapse -->
+
-
          </div><!-- /.navbar-inner -->
+
-
        </div><!-- /.navbar -->
+
-
 
+
-
      </div> <!-- /.container -->
+
-
    </div><!-- /.navbar-wrapper -->
+
-
   
+
-
    <!--link circles-->
+
-
<div id="nav-circles">
+
-
<ul class="nav">
+
          
          
-
<a href="#jumbotron"><li></li></a>
 
-
<a href="#first-section"><li></li></a>
 
-
<a href="#second-section"><li></li></a>
 
-
<a href="#third-section"><li></li></a>
 
-
</ul>
 
-
    </div>
 
-
 
-
    <!--Giant Image At the Top-->
 
-
    <div class="jumbotron masthead">
 
-
  <div class="container">
 
-
    <h1>Project</h1>
 
-
    <p>Lorem ipsum dolor sit amet.</p>
 
-
    <p>
 
-
  </div>
 
-
</div>
 
      
      
-
<div class="img-span">
 
-
</div>
 
-
 
-
    <!--First section-->
 
-
    <div class="section" id="first-section">
 
          
          
-
     <div class="row">
+
     </style>
-
      <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/jtmF8YkJ_P/device%20photo.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 5%;">
+
</head>
-
        </div><!-- /.span4 -->
+
<body>
-
    <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/WTlON78GgW/IMG_0264.JPG?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 15%;">
+
<img src="http://upload.wikimedia.org/wikipedia/en/d/d6/IGEM_official_logo.png" id="igem"/><!--igem logo-->
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/WTlON78GgW/IMG_0264.JPG?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
+
<img src="http://collegediabetesnetwork.org/wp-content/uploads/2012/07/UPenn_logo1.png" id="penn"/> <!--penn logo-->
-
         </div><!-- /.span4 -->
+
  <div class="left_wrap">
 +
    </div> </div>
 +
  <div class="section1" style="background-position: top;">
 +
         <div class="text">
 +
            <b><center><h1>
 +
Project Description
 +
</b></center></h1>
 +
<br>
 +
The code of life is more than a sequence of A’s, C’s, T’s and G’s; epigenetic modifications, such as DNA methylation, are powerful and heritable regulators of gene expression. Targeted methyltransferases are enzymes that catalyze sequence-specific methylation – the most useful tool for engineering the epigenome. With a synthetic biology approach, we developed an assay to test targeted methyltransferases without expensive, time-consuming traditional methods. Our modular single-plasmid system allows methyltransferases to be easily cloned and tested via inexpensive digestion assays, quickly measuring the existence and extent of targeted methylation. Additionally, our plasmid contains standardized primer-binding sites for methylation-sensitive sequencing, and our E. coli chassis effectively eliminated noise associated with methylation studies. We are using this assay to characterize our novel targeted methyltransferases, which could be used to study epigenetic modifications. In the future, synthetic biologists could embrace these tools to explore the next frontier in engineering biological systems: the epigenome.</center>
 +
<br><br>
-
<div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/Y5d0PLu26o/photo-7.JPG?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg');left: 30%;">
 
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/APrj2Se2i-/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2811.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
 
-
        </div><!-- /.span4 --></div>
 
-
     
+
         </div>
-
     
+
-
 
+
-
    <!--first hero unit-->
+
-
    <div class="hero-unit" id="text">
+
-
         <h4>Abstract</h4>
+
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
    </div><div class="hero-unit" id="text">
+
-
                                <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                                        </div><div class="hero-unit" id="text">
+
-
                                        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                                                <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                                                        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                                                                <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
+
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
+
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
+
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
+
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
+
-
                                                               
+
     </div>
     </div>
-
</div>
 
-
 
-
      </div>
 
      
      
-
<div class="img-span">
 
-
</div>
 
-
   
 
-
    <div class="section" id="second-section">
 
-
       
 
-
           
 
-
    <div class="row">
 
-
      <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/0RorW85BOS/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2733.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 5%;">
 
-
        </div><!-- /.span4 -->
 
-
    <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/8auH76HSpC/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2721.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 15%;">
 
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/WTlON78GgW/IMG_0264.JPG?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
 
-
        </div><!-- /.span4 -->
 
-
<div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/Cqv03LbbM-/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2809.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg');left: 30%;">
 
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/APrj2Se2i-/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2811.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
 
-
        </div><!-- /.span4 --></div>
 
-
 
-
 
-
     
 
-
    <!--first hero unit-->
 
-
    <div class="hero-unit" id="text">
 
-
        <h4>Methods</h4>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
    </div>
 
-
   
 
-
    </div> <!--endsection-->
 
-
   
 
-
        </div>
 
-
   
 
-
   
 
-
<div class="img-span">
 
-
</div>
 
-
   
 
-
    <div class="section" id="third-section">
 
-
       
 
-
        <div class="row">
 
-
      <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/FsZxKlaNDL/Screen%20shot%202013-05-31%20at%2015.29.53.png?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 5%;">
 
-
        </div><!-- /.span4 -->
 
-
    <div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/-RV-MArbh8/Screen%20shot%202013-05-29%20at%2016.22.26.png?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg'); left: 15%;">
 
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/WTlON78GgW/IMG_0264.JPG?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
 
-
        </div><!-- /.span4 -->
 
-
 
-
<div class="span4" style="background-image: url('https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/-RV-MArbh8/Screen%20shot%202013-05-29%20at%2016.22.26.png?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg');left: 30%;">
 
-
          <!--<img src="https://dl.dropboxusercontent.com/sh/h4cxid18rzzjgan/APrj2Se2i-/Lynch%20Labs%20PGFI/BE_LynchLabGroup_DSC2811.jpg?token_hash=AAGlfzG2xZpKrOTI8pyH02EbYxY1vK3QLUWKsWuYdaPbYg"/>-->
 
-
        </div><!-- /.span4 --></div>
 
-
       
 
-
       
 
-
 
-
    <!--first hero unit-->
 
-
    <div class="hero-unit" id="text">
 
-
        <h4>Results</h4>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
    </div>
 
-
   
 
-
    </div> <!--endsection-->
 
-
   
 
-
   
 
-
    <div class="hero-unit" id="text">
 
-
        <h4>Results 2</h4>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
        <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor
 
-
  incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud
 
-
  exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor
 
-
  in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur
 
-
  sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p>
 
-
    </div>
 
-
   
 
-
    </div> <!--endsection--> 
 
-
       
 
-
    </body>
 
-
 
 
-
 
 
    
    
 +
</body>
 +
<script>
 +
</script>br/
</html>
</html>

Latest revision as of 03:50, 28 September 2013

modeling

Project Description


The code of life is more than a sequence of A’s, C’s, T’s and G’s; epigenetic modifications, such as DNA methylation, are powerful and heritable regulators of gene expression. Targeted methyltransferases are enzymes that catalyze sequence-specific methylation – the most useful tool for engineering the epigenome. With a synthetic biology approach, we developed an assay to test targeted methyltransferases without expensive, time-consuming traditional methods. Our modular single-plasmid system allows methyltransferases to be easily cloned and tested via inexpensive digestion assays, quickly measuring the existence and extent of targeted methylation. Additionally, our plasmid contains standardized primer-binding sites for methylation-sensitive sequencing, and our E. coli chassis effectively eliminated noise associated with methylation studies. We are using this assay to characterize our novel targeted methyltransferases, which could be used to study epigenetic modifications. In the future, synthetic biologists could embrace these tools to explore the next frontier in engineering biological systems: the epigenome.

br/

Retrieved from "http://2013.igem.org/Team:Penn/Project"