Template:Kyoto/Notebook/Sep 6

From 2013.igem.org

(Difference between revisions)

Latest revision as of 03:56, 28 September 2013

Sep 6

Electrophoresis

Hirano

Lane	Sample
1	100bp ladder
2	9/4 RBS-lysis3-DT -3
3	9/4 RBS-lysis3-DT -4
4	9/4 RBS-lysis3-DT -5
5	9/4 RBS-lysis1-DT -3
6	9/4 Ptrc-KaiC -1
7	9/4 pSB4K5 -1
8	100bp ladder

Colony PCR

No name

Sample	base pair
Pconst-spinach-DT-(1~4)	563
Plac-spinach-DT-(1~4)	659
Pcon-pT181 antisense-(1~4)	468
Ptet-pT181 antisense-(1~4)	604
Pconst-pT181 attenuator-(1~4)	568
Pconst+RBS-tetR-DT-(1~4)	1121
Ptet+RBS-lacZα-DT-(1~4)	1162

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	40s	30cycles

Liquid Culture

No name

Sample	medium
9/4 RBS-Lysis3+DT3	Plusgrow medium(+CP)
9/4 RBS-Lysis1+DT3	Plusgrow medium(+CP)
9/4 pSB4K5	Plusgrow medium(+Kan)

Electrophoresis

No name

Lane	Sample
1	100bp ladder
2	Pconst-spinach-DT -(1)
3	Pconst-spinach-DT -(2)
4	Pconst-spinach-DT -(3)
5	Pconst-spinach-DT -(4)
6	Plac-spinach-DT -(1)
7	Plac-spinach-DT -(2)
8	Plac-spinach-DT -(3)
9	Plac-spinach-DT -(4)
10	Pcon-pT181 antisense -(1)
11	Pcon-pT181 antisense -(2)
12	Pcon-pT181 antisense -(3)
13	Pcon-pT181 antisense -(4)

Lane	Sample
1	100bp ladder
2	Ptet-pT181 antisense -(1)
3	Ptet-pT181 antisense -(2)
4	Ptet-pT181 antisense -(3)
5	Ptet-pT181 antisense -(4)
6	Plac-pT181 attenuator -(1)
7	Plac-pT181 attenuator -(2)
8	Plac-pT181 attenuator -(3)
9	Plac-pT181 attenuator -(4)

Miniprep

Honda and Kojima

DNA	concentration [µg/mL]	260/280	260/230
pT181 antisense-(1)	238.7	1.68	1.44
pT181 antisense-(2)	248.8	1.83	1.77
pT181 antisense-(3)	223.2	1.78	1.61
pT181 antisense-(4)	220.8	1.89	1.92
tetR aptamer-(1)	252.5	1.89	1.90
tetR aptamer-(2)	211.2	1.90	1.99
DT181 attenuator	102.2	1.98	2.17
121R aptamer-DT	251.7	1.92	2.01
pSB4K5	212.9	1.80	1.90
RBS-lacZα-DT	185.7	1.78	1.79
RBS-lysis2-DT-(1)	84.8	1.85	2.24

Restriction Enzyme Digestion

Tatsui

	9/6 RBS-lysis2-DT (78.3 µg/mL)	XbaI	PstI	BSA	Buffer	MilliQ	total
2 cuts	22 µL	1 µL	1 µL	3 µL	3 µL	0 µL	30 µL
NC	1.3 µL	0 µL	0 µL	1 µL	1 µL	6.7 µL	10 µL

	aptamer-DT (251.7 µg/mL)	XbaI	PstI	BSA	Buffer	MilliQ	total
2 cuts	0.4 µL	1 µL	1 µL	3 µL	3 µL	14.1 µL	30 µL
NC	0.4 µL	0 µL	0 µL	1 µL	1 µL	7.6 µL	10 µL

	Pλ-luxI (86.4 µg/mL)	EcoRI	SpeI	BSA	Buffer	MilliQ	total
2 cuts	23.1 µL	1 µL	1 µL	3 µL	3 µL	1.9 µL	30 µL
NC	1.2 µL	0 µL	0 µL	1 µL	1 µL	6.8 µL	10 µL

at 37 °C, for 1h

Electrophoresis

Hirano

Lane	Sample
1	100bp ladder
2	Pconst+RBS-tetR-DT -(1)
3	Pconst+RBS-tetR-DT -(2)
4	Pconst+RBS-tetR-DT -(3)
5	Pconst+RBS-tetR-DT -(4)
6	Ptet+RBS-lacZα-DT -(1)
7	Ptet+RBS-lacZα-DT -(2)
8	100bp ladder
9	Ptet+RBS-lacZα-DT -(3)
10	Ptet+RBS-lacZα-DT -(4)
11	100bp ladder

Gel Extraction

No name

Lane	DNA	Enzyme
1	100bp ladder	--
2	RBS-lysis2-DT	XbaI & PstI
3	RBS-lysis2-DT	XbaI & PstI
4	--	--
5	aptamer 12_1R-DT	XbaI & PstI
6	aptamer 12_1R-DT	XbaI & PstI

Lane	DNA	Enzyme
1	100bp ladder	--
2	Pλ-luxI	EcoRI & SpeI
3	Pλ-luxI	EcoRI & SpeI

Name	concentration[µg/mL]	260/280	260/230
RBS-lysis2-DT (XbaI & PstI)	7.1	1.88	0.07
aptamer 12_1R-DT (XbaI & PstI)	15.3	1.93	0.70
Pλ-luxI (EcoRI & SpeI)	4.5	1.79	0.31

Liquid Culture

No name

Sample	medium
9/5 Pconst-spinach-DT-(1)	Plusgrow medium(+Amp&CP)
9/5 Ptet-pT181 antisense-(1)	Plusgrow medium(+CP)
9/5 Pconst+RBS-tetR-DT-(1)	Plusgrow medium(+Amp&CP)
9/5 Ptet+RBS-lacZα-DT-(1)	Plusgrow medium(+CP)
9/5 Pcon-pT181 attenuator-(1)	Plusgrow medium(+CP)
9/4 pT181 antisense-(1)	Plusgrow medium(+CP)
9/4 aptamer 12_1R-(1)	Plusgrow medium(+CP)

Colony PCR

No name

Sample	base pair
9/5 Plac-spinach-DT-(5~12)	563
9/5 Pcon-antisense-(5~12)	468
9/5 Plac-attenuator-(5~12)	664
9/5 pT181 antisense+Plac-(1)	-

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	42s	30cycles

@@ Line 1: / Line 1: @@
 ==Sep 6==
-===Liquid Culture===
+===Electrophoresis===
-<!-- ここから -->
 <div class="experiment">
-<span class="author">No name</span>
+<span class="author">Hirano</span>
 {| class="wikitable"
-!Sample||medium
+!Lane||Sample
+|-
+|1||100bp ladder
+|-
+|2||9/4 RBS-lysis3-DT -3
+|-
+|3||9/4 RBS-lysis3-DT -4
+|-
+|4||9/4 RBS-lysis3-DT -5
+|-
+|5||9/4 RBS-lysis1-DT -3
 |-
-|9/4 RBS-lysis3＋DT3 ||Plusgrow medium(+CP)
+|6||9/4 Ptrc-KaiC -1
 |-
-|9/4 RBS-lysis1＋DT3 ||　Plusgrow medium(+CP)
+|7||9/4 pSB4K5 -1
 |-
-|9/4 psB4K5||Plusgrow medium(+K)
+|8||100bp ladder
 |}
+[[File:Igku Sep6 ColonyPCRN1.jpg]]<br>
 </div>
@@ Line 21: / Line 31: @@
 !|Sample||base pair
 |-
-|Pconst-spinach-DT-(1)||-
+|Pconst-spinach-DT-(1~4)||563
 |-
-|Pconst-spinach-DT-(2)||-
+|Plac-spinach-DT-(1~4)||659
 |-
-|Pconst-spinach-DT-(3)||-
+|Pcon-pT181 antisense-(1~4)||468
 |-
-|Pconst-spinach-DT-(4)||-
+|Ptet-pT181 antisense-(1~4)||604
 |-
-|Plac-spinach-DT-(1)||-
+|Pconst-pT181 attenuator-(1~4)||568
 |-
-|Plac-spinach-DT-(2)||-
+|Pconst+RBS-tetR-DT-(1~4)||1121
 |-
-|Plac-spinach-DT-(3)||-
+|Ptet+RBS-lacZ&alpha;-DT-(1~4)||1162
+|}
+{| class="wikitable"
+!PreDenature||Denature||Annealing||Extension||cycle
 |-
-|Plac-spinach-DT-(4)||-
+|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 |-
-|Pcon-PT181-antisense-(1)||-
+|5min||30s||30s||40s||30cycles
+|}
+</div>
+===Liquid Culture===
+<div class="experiment">
+<span class="author">No name</span>
+{| class="wikitable"
+!Sample||medium
 |-
-|Pcon-PT181-antisense-(2)||-
+|9/4 RBS-Lysis3+DT3||Plusgrow medium(+CP)
 |-
-|Pcon-PT181-antisense-(3)||-
+|9/4 RBS-Lysis1+DT3||Plusgrow medium(+CP)
 |-
-|Pcon-PT181-antisense-(4)||-
+|9/4 pSB4K5||Plusgrow medium(+Kan)
 |}
+</div>
+===Electrophoresis===
+<div class="experiment">
+<span class="author">No name</span>
 {| class="wikitable"
-!|Sample||base pair
+!Lane||Sample
 |-
-|Ptet-PT181-antisense-(1)||-
+|1||100bp ladder
 |-
-|Ptet-PT181-antisense-(2)||-
+|2||Pconst-spinach-DT -(1)
 |-
-|Ptet-PT181-antisense-(3)||-
+|3||Pconst-spinach-DT -(2)
 |-
-|Ptet-PT181-antisense-(4)||-
+|4||Pconst-spinach-DT -(3)
 |-
-|Plac-PT181-antisense-(1)||-
+|5||Pconst-spinach-DT -(4)
 |-
-|Plac-PT181-antisense-(2)||-
+|6||Plac-spinach-DT -(1)
 |-
-|Plac-PT181-antisense-(3)||-
+|7||Plac-spinach-DT -(2)
 |-
-|Plac-PT181-antisense-(4)||-
+|8||Plac-spinach-DT -(3)
-|}
-===Miniprep===
-<div class="experiment">
-<span class="author">No name</span>
-{|class="wikitable"
-!DNA||concentration[&micro;g/mL]||260/280||260/230
 |-
-|(・▽・)||　||　||
+|9||Plac-spinach-DT -(4)
+|-
+|10||Pcon-pT181 antisense -(1)
+|-
+|11||Pcon-pT181 antisense -(2)
+|-
+|12||Pcon-pT181 antisense -(3)
+|-
+|13||Pcon-pT181 antisense -(4)
 |}
-</div>
-<!-- ここまでをコピーしてね -->
-===Restriction Enzyme Digestion===
-<div class="experiment">
-<span class="author">Murata, Murakami, Nakamoto, Ashida, Matsumoto and Yoshida</span>
 {| class="wikitable"
-! ||8/11 J23100 -(2)||SpeI||PstI||Buffer||MilliQ||total
+!Lane||Sample
 |-
-|2 cuts||4.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||21&micro;L||30&micro;L
+|1||100bp ladder
 |-
-|1 cut||1.3&micro;L||0.2&micro;L||--||1.0&micro;L||7.5&micro;L||10&micro;L
+|2||Ptet-pT181 antisense -(1)
 |-
-|1 cut||1.3&micro;L||--||0.2&micro;L||1.0&micro;L||7.5&micro;L||10&micro;L
+|3||Ptet-pT181 antisense -(2)
 |-
-|NC||1.3&micro;L||--||--||1.0&micro;L||7.7&micro;L||10&micro;L
+|4||Ptet-pT181 antisense -(3)
-|}
-*at 37&deg;C, for 1h
-{| class="wikitable"
-! ||8/15 B0034(RBS) -(2)||SpeI||PstI||Buffer||MilliQ||total
 |-
-|2 cuts||6.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||19&micro;L||30&micro;L
+|5||Ptet-pT181 antisense -(4)
 |-
-|1 cut||2.0&micro;L||0.2&micro;L||--||1.0&micro;L||6.8&micro;L||10&micro;L
+|6||Plac-pT181 attenuator -(1)
 |-
-|1 cut||2.0&micro;L||--||0.2&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|7||Plac-pT181 attenuator -(2)
 |-
-|NC||2.0&micro;L||--||--||1.0&micro;L||7.0&micro;L||10&micro;L
+|8||Plac-pT181 attenuator -(3)
+|-
+|9||Plac-pT181 attenuator -(4)
 |}
-*at 37&deg;C, for 1h
+[[File:igku_Sep6_COlonyPCRN1_1a.jpg]]<br>
-{| class="wikitable"
+[[File:igku_Sep6_colonyPCR_N2_1b.jpg]]<br>
-! ||8/17 K117000(lysis1) -(1)||XbaI||PstI||BSA||Buffer||MilliQ||total
+</div>
+===Miniprep===
+<div class="experiment">
+<span class="author">Honda and Kojima</span>
+{|class="wikitable"
+!DNA||concentration [&micro;g/mL]||260/280||260/230
 |-
-|2 cuts||2.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||3.0&micro;L||20&micro;L||30&micro;L
+|pT181 antisense-(1)||238.7||1.68||1.44
 |-
-|1 cut||1.0&micro;L||0.2&micro;L||--||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|pT181 antisense-(2)||248.8||1.83||1.77
 |-
-|1 cut||1.0&micro;L||--||0.2&micro;L||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|pT181 antisense-(3)||223.2||1.78||1.61
 |-
-|NC||1.0&micro;L||--||--||1.0&micro;L||1.0&micro;L||7.0&micro;L||10&micro;L
+|pT181 antisense-(4)||220.8||1.89||1.92
-|}
-*at 37&deg;C, for 1h
-{| class="wikitable"
-! ||8/17 K748002(lysis3) -(1)||XbaI||PstI||BSA||Buffer||MilliQ||total
 |-
-|2 cuts||2.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||3.0&micro;L||20&micro;L||30&micro;L
+|tetR aptamer-(1)||252.5||1.89||1.90
 |-
-|1 cut||1.0&micro;L||0.2&micro;L||--||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|tetR aptamer-(2)||211.2||1.90||1.99
 |-
-|1 cut||1.0&micro;L||--||0.2&micro;L||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|DT181 attenuator||102.2||1.98||2.17
 |-
-|NC||1.0&micro;L||--||--||1.0&micro;L||1.0&micro;L||7.0&micro;L||10&micro;L
+|121R aptamer-DT||251.7||1.92||2.01
+|-
+|pSB4K5||212.9||1.80||1.90
+|-
+|RBS-lacZ&alpha;-DT||185.7||1.78||1.79
+|-
+|RBS-lysis2-DT-(1)||84.8||1.85||2.24
 |}
-*at 37&deg;C, for 1h
+</div>
+===Restriction Enzyme Digestion===
+<div class="experiment">
+<span class="author">Tatsui</span>
 {| class="wikitable"
-! ||8/17 I13504(RBS-GFP-DT) -(1)||XbaI||PstI||BSA||Buffer||MilliQ||total
+! ||9/6 RBS-lysis2-DT (78.3 &micro;g/mL)||XbaI||PstI||BSA||Buffer||MilliQ||total
 |-
-|2 cuts||2.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||3.0&micro;L||20&micro;L||30&micro;L
+|2 cuts||22 &micro;L||1 &micro;L||1 &micro;L||3 &micro;L||3 &micro;L||0 &micro;L||30 &micro;L
 |-
-|1 cut||1.0&micro;L||0.2&micro;L||--||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|NC||1.3 &micro;L||0 &micro;L||0 &micro;L||1 &micro;L||1 &micro;L||6.7 &micro;L||10 &micro;L
+|}
+{| class="wikitable"
+! ||aptamer-DT (251.7 &micro;g/mL)||XbaI||PstI||BSA||Buffer||MilliQ||total
 |-
-|1 cut||1.0&micro;L||--||0.2&micro;L||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|2 cuts||0.4 &micro;L||1 &micro;L||1 &micro;L||3 &micro;L||3 &micro;L||14.1 &micro;L||30 &micro;L
 |-
-|NC||1.0&micro;L||--||--||1.0&micro;L||1.0&micro;L||7.0&micro;L||10&micro;L
+|NC||0.4 &micro;L||0 &micro;L||0 &micro;L||1 &micro;L||1 &micro;L||7.6 &micro;L||10 &micro;L
 |}
-*at 37&deg;C, for 1h
 {| class="wikitable"
-! ||8/17 I0462(RBS-luxR-DT) -(1)||XbaI||PstI||BSA||Buffer||MilliQ||total
+! ||P&lambda;-luxI (86.4 &micro;g/mL)||EcoRI||SpeI||BSA||Buffer||MilliQ||total
 |-
-|2 cuts||2.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||3.0&micro;L||20&micro;L||30&micro;L
+|2 cuts||23.1 &micro;L||1 &micro;L||1 &micro;L||3 &micro;L||3 &micro;L||1.9 &micro;L||30 &micro;L
 |-
-|1 cut||1.0&micro;L||0.2&micro;L||--||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|NC||1.2 &micro;L||0 &micro;L||0 &micro;L||1 &micro;L||1 &micro;L||6.8 &micro;L||10 &micro;L
-|-
-|1 cut||1.0&micro;L||--||0.2&micro;L||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
-|-
-|NC||1.0&micro;L||--||--||1.0&micro;L||1.0&micro;L||7.0&micro;L||10&micro;L
 |}
-*at 37&deg;C, for 1h
+*at 37 &deg;C, for 1h
+===Electrophoresis===
+<div class="experiment">
+<span class="author">Hirano</span>
 {| class="wikitable"
-! ||8/17 I732020(RBS-lacZ&alpha;-DT) -(1)||XbaI||PstI||BSA||Buffer||MilliQ||total
+!Lane||Sample
 |-
-|2 cuts||2.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||3.0&micro;L||20&micro;L||30&micro;L
+|1||100bp ladder
 |-
-|1 cut||1.0&micro;L||0.2&micro;L||--||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|2||Pconst+RBS-tetR-DT -(1)
 |-
-|1 cut||1.0&micro;L||--||0.2&micro;L||1.0&micro;L||1.0&micro;L||6.8&micro;L||10&micro;L
+|3||Pconst+RBS-tetR-DT -(2)
 |-
-|NC||1.0&micro;L||--||--||1.0&micro;L||1.0&micro;L||7.0&micro;L||10&micro;L
+|4||Pconst+RBS-tetR-DT -(3)
-|}
-*at 37&deg;C, for 1h
-{| class="wikitable"
-! ||8/11 R6061 -(1)||SpeI||PstI||Buffer||MilliQ||total
 |-
-|2 cuts||8.0&micro;L||1.0&micro;L||1.0&micro;L||3.0&micro;L||17&micro;L||30&micro;L
+|5||Pconst+RBS-tetR-DT -(4)
 |-
-|1 cut||3.0&micro;L||0.2&micro;L||--||1.0&micro;L||5.8&micro;L||10&micro;L
+|6||Ptet+RBS-lacZ&alpha;-DT -(1)
 |-
-|1 cut||3.0&micro;L||--||0.2&micro;L||1.0&micro;L||5.8&micro;L||10&micro;L
+|7||Ptet+RBS-lacZ&alpha;-DT -(2)
 |-
-|NC||3.0&micro;L||--||--||1.0&micro;L||6.0&micro;L||10&micro;L
+|8||100bp ladder
+|-
+|9||Ptet+RBS-lacZ&alpha;-DT -(3)
+|-
+|10||Ptet+RBS-lacZ&alpha;-DT -(4)
+|-
+|11||100bp ladder
 |}
-*at 37&deg;C, for 1h
+[[File:igku_Sep6_electrophoresis_N3.jpg]]<br>
 </div>
 ===Gel Extraction===
 <div class="experiment">
-<span class="author">Ashida</span>
+<span class="author">No name</span>
 {| class="wikitable"
 !Lane||DNA||Enzyme
@@ Line 185: / Line 224: @@
 |1||100bp ladder||--
 |-
-|3||8/18 K117000(lysis2) -1||XbaI & PstI
+|2||rowspan="2"|RBS-lysis2-DT||rowspan="2"|XbaI & PstI
+|-
+|3
+|-
+|4||--||--
+|-
+|5||rowspan="2"|aptamer 12_1R-DT||rowspan="2"|XbaI & PstI
 |-
-|5||8/18 K117000(lysis2) -1||XbaI & PstI
+|6
 |}
-[[File:igku_Aug19electrophoresis3]]<br>
-[[File:igku_Aug19electrophoresis4]]<br>
 {| class="wikitable"
-!Name||concentration[&micro;g/mL]||260/280||260/230
+!Lane||DNA||Enzyme
 |-
-|8/18 lysis2-1(XbaI & PstI)||3.1||3.64||0.19
+|1||100bp ladder||--
 |-
-|8/18 lysis2-1(XbaI & PstI)||3.4||1.89||0.13
+|2||rowspan="2"|P&lambda;-luxI||rowspan="2"|EcoRI & SpeI
+|-
+|3
 |}
+[[File:igku_Sep6_gel_extraction_N4_2.jpg]]<br>
-</div>
+[[File:igku_Sep6_gel_extraction_N4_3.jpg]]<br>
-===Liquid Culture===
-<div class="experiment">
-<span class="author">Nakamoto</span>
 {| class="wikitable"
-!Sample||medium
+!Name||concentration[&micro;g/mL]||260/280||260/230
 |-
-|8/15 BBa_K112806(lysis2) -(1)||Plusgrow medium(+CP)
+|RBS-lysis2-DT (XbaI & PstI)||7.1||1.88||0.07
 |-
-|8/15 BBa_Q04400(tetR) -(1)||Plusgrow medium(+Amp)
+|aptamer 12_1R-DT (XbaI & PstI)||15.3||1.93||0.70
 |-
+|P&lambda;-luxI (EcoRI & SpeI)||4.5||1.79||0.31
 |}
+[[File:igku_Sep6_gel_extraction_N5_1.jpg]]<br>
+[[File:igku_Sep6_gel_extraction_N5_2.jpg]]<br>
 </div>
+===Liquid Culture===
-===Colony PCR===
 <div class="experiment">
-<span class="author">Nakamoto</span>
+<span class="author">No name</span>
 {| class="wikitable"
-!|Sample||base pair
+!Sample||medium
 |-
-|8/15 BBa_K112806(lysis2) -(1)||828
+|9/5 Pconst-spinach-DT-(1)||Plusgrow medium(+Amp&CP)
 |-
-|negative control||--
+|9/5 Ptet-pT181 antisense-(1)||Plusgrow medium(+CP)
-|}
-{| class="wikitable"
-!PreDenature||Denature||Annealing||Extension||cycle
 |-
-|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+|9/5 Pconst+RBS-tetR-DT-(1)||Plusgrow medium(+Amp&CP)
 |-
-|5min||30s||30s||1min||30cycles
+|9/5 Ptet+RBS-lacZ&alpha;-DT-(1)||Plusgrow medium(+CP)
-|}
-{| class="wikitable"
-!Sample||base pair
 |-
-|8/10 BBa_Q0400 -(1)||3995
+|9/5 Pcon-pT181 attenuator-(1)||Plusgrow medium(+CP)
 |-
-|negative control||--
+|9/4 pT181 antisense-(1)||Plusgrow medium(+CP)
-|}
-{| class="wikitable"
-!PreDenature||Denature||Annealing||Extension||cycle
 |-
-|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+|9/4 aptamer 12_1R-(1)||Plusgrow medium(+CP)
-|-
-|5min||30s||30s||30s||30cycles
 |}
+</div>
 ===Colony PCR===
 <div class="experiment">
-<span class="author">Nakamoto</span>
+<span class="author">No name</span>
 {| class="wikitable"
 !|Sample||base pair
 |-
-|8/15 BBa_K112806(lysis2) -(1)||828
+|9/5 Plac-spinach-DT-(5~12)||563
 |-
-|negative control||--
+|9/5 Pcon-antisense-(5~12)||468
-|}
-{| class="wikitable"
-!PreDenature||Denature||Annealing||Extension||cycle
 |-
-|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+|9/5 Plac-attenuator-(5~12)||664
 |-
-|5min||30s||30s||1min||30cycles
+|9/5 pT181 antisense+Plac-(1)||-
-|}
-{| class="wikitable"
-!Sample||base pair
-|-
-|8/10 BBa_Q0400 -(1)||3995
-|-
-|negative control||--
 |}
 {| class="wikitable"
@@ Line 277: / Line 299: @@
 |94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 |-
-|5min||30s||30s||30s||30cycles
+|5min||30s||30s||42s||30cycles
 |}
+</div>

Template:Kyoto/Notebook/Sep 6

From 2013.igem.org

Latest revision as of 03:56, 28 September 2013

Contents

Sep 6

Electrophoresis

Colony PCR

Liquid Culture

Electrophoresis

Miniprep

Restriction Enzyme Digestion

Electrophoresis

Gel Extraction

Liquid Culture

Colony PCR