Team:Northwestern
From 2013.igem.org
SamsonFong (Talk | contribs) |
SamsonFong (Talk | contribs) |
||
(47 intermediate revisions not shown) | |||
Line 18: | Line 18: | ||
/* Thanks to Matthew Mombrea for the following code!! Link: http://www.itworld.com/development/354027/how-build-drop-down-menu-css-and-html"*/ | /* Thanks to Matthew Mombrea for the following code!! Link: http://www.itworld.com/development/354027/how-build-drop-down-menu-css-and-html"*/ | ||
- | #main-nav {width: | + | #main-nav {width: 850px;height: 50px;background: #C3C3E5; margin:auto; margin-top: 0px; border-radius: 20px} |
#main-nav .subnav { | #main-nav .subnav { | ||
display: none; | display: none; | ||
Line 75: | Line 75: | ||
/** END OF HEADER **/ | /** END OF HEADER **/ | ||
+ | body { | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | background: #222; | ||
+ | color: #EEE; | ||
+ | text-align: center; | ||
+ | font: normal 9pt Verdana; | ||
+ | } | ||
+ | a:link, a:visited { | ||
+ | color: #EEE; | ||
+ | } | ||
+ | img { | ||
+ | border: none; | ||
+ | } | ||
+ | #normal2, #normal3, #normal4, #normal5 { | ||
+ | display: none; | ||
+ | } | ||
+ | #gallery { | ||
+ | margin: 0 auto; | ||
+ | width: 800px; | ||
+ | } | ||
+ | #thumbs { | ||
+ | margin: 10px auto 10px auto; | ||
+ | text-align: center; | ||
+ | width: 800px; | ||
+ | } | ||
+ | #bigimages { | ||
+ | width: 770px; | ||
+ | float: left; | ||
+ | } | ||
+ | #thumbs img { | ||
+ | width: 130px; | ||
+ | height: 130px; | ||
+ | } | ||
+ | #bigimages img { | ||
+ | border: 4px solid #555; | ||
+ | margin-top: 5px; | ||
+ | width: 750px; | ||
+ | } | ||
+ | #thumbs a:link, #thumbs a:visited { | ||
+ | width: 130px; | ||
+ | height: 130px; | ||
+ | border: 6px solid #555; | ||
+ | margin: 6px; | ||
+ | float: left; | ||
+ | } | ||
+ | #thumbs a:hover { | ||
+ | border: 6px solid #888; | ||
+ | } | ||
- | + | /**END of gallery**/ | |
- | + | ||
- | + | ||
#picture { | #picture { | ||
Line 95: | Line 142: | ||
#project-description { | #project-description { | ||
- | width: | + | width: 850px; |
- | height: | + | height: 500px; |
- | + | margin : auto; | |
display: block; | display: block; | ||
font-size: 16px; | font-size: 16px; | ||
font-family: "Georgia"; | font-family: "Georgia"; | ||
+ | text-align: left; | ||
} | } | ||
.first-row { | .first-row { | ||
width: 100%; | width: 100%; | ||
- | height: | + | height: 350px; |
display: block; | display: block; | ||
background-color: #C972D0; | background-color: #C972D0; | ||
Line 209: | Line 257: | ||
border-radius: 20px} | border-radius: 20px} | ||
+ | p{ | ||
+ | text-indent: 40px;} | ||
</style> | </style> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
+ | <script type="text/javascript"> | ||
+ | |||
+ | function changeImage(current) { | ||
+ | var imagesNumber = 5; | ||
+ | |||
+ | for (i=1; i<=imagesNumber; i++) { | ||
+ | if (i == current) { | ||
+ | document.getElementById("normal" + current).style.display = "block"; | ||
+ | } else { | ||
+ | document.getElementById("normal" + i).style.display = "none"; | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | |||
+ | </script> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | + | </br> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
+ | <body> | ||
- | <p id = "project-description-title"> <font size=1>N</font> <font size=2>N</font> <font size=3>N</font> <font size=4>N</font> <font size=5>N</font> NUtralize! </p> | + | <div id="gallery"> |
+ | <div id="bigimages"> | ||
+ | <div id="normal1"> | ||
+ | <a href = "https://2013.igem.org/Team:Northwestern/Project"> <img src="https://static.igem.org/mediawiki/2013/c/cc/NorthwesternDual-state-gallery.gif" alt=""/> </a> | ||
+ | </div> | ||
+ | |||
+ | <div id="normal2"> | ||
+ | <a href = "https://2013.igem.org/Team:Northwestern/Human_Practice"> <img src="https://static.igem.org/mediawiki/2013/1/1c/NorthwesternHuman-practices-gallery.gif" alt=""/> </a> | ||
+ | </div> | ||
+ | |||
+ | <div id="normal3"> | ||
+ | <a href = "https://2013.igem.org/Team:Northwestern/Team"> <img src="https://static.igem.org/mediawiki/2013/8/89/Meet-the-team.gif" alt=""/> </a> | ||
+ | </div> | ||
+ | |||
+ | <div id="normal4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/3/39/NorthwesternOral-health-gallery.gif" alt=""/> | ||
+ | </div> | ||
+ | |||
+ | <div id="normal5"> | ||
+ | <a href = "https://2013.igem.org/Team:Northwestern/methods"> <img src="https://static.igem.org/mediawiki/2013/7/73/NorthwesternMETHODS-GALLERY.gif" alt=""/> </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="thumbs"> | ||
+ | <a href="javascript: changeImage(1);"><img src="https://static.igem.org/mediawiki/2013/e/ef/Dual-state-gallery-small.gif" alt="" /></a> | ||
+ | <a href="javascript: changeImage(2);"><img src="https://static.igem.org/mediawiki/2013/1/13/NorthwesternHuman-practices-gallery-small.gif" alt="" /></a> | ||
+ | <a href="javascript: changeImage(3);"><img src="https://static.igem.org/mediawiki/2013/b/b6/Meet-the-team-small.gif" alt="" /></a> | ||
+ | <a href="javascript: changeImage(4);"><img src="https://static.igem.org/mediawiki/2013/4/4b/Oral-health-gallery-small.gif" alt="" /></a> | ||
+ | <a href="javascript: changeImage(5);"><img src="https://static.igem.org/mediawiki/2013/b/b5/METHODS-GALLERY-small.gif" alt="" /></a> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | |||
+ | <p id = "project-description-title"> <!--<font size=1>N</font> <font size=2>N</font> <font size=3>N</font> <font size=4>N</font> <font size=5>N</font>--> NUtralize! </p> | ||
<div id = "project-description"> | <div id = "project-description"> | ||
- | + | <p> Poor oral health (tooth decay and infection) can lead to many painful symptoms and complicated treatments. Mouth bacteria are a primary culprit in tooth decay, as lactic acid secreted by natural bacteria in the human mouth decreases pH, leading to cavity formation. The enamel demineralization threshold is known to be around pH 5.5. The overall vision of our project is to prevent mouth pH from falling below 5.5 by engineering bacteria to sense pH and respond by increasing the alkalinity. To do this, we aimed to create genetic pH sensing elements that would work in the mouth. <p/> | |
+ | <p>Our project laid the foundation for a dual-state promoter consisting of two promoters in series: a constitutive promoter active at a (tunable) basal level and an upstream, pH-inducible promoter. Previous research has found that the E. coli genes Asr and GadA are highly expressed when pH drops below 5.5. This research drove us to choose the promoter elements for GadA and Asr as our pH-inducible promoters. </p> | ||
+ | <p>We have characterized a new pH-responsive promoter (Asr) as well as an iGEM-characterized promoter (GadA) and a constitutive promoter (Lpp). Ongoing work will evaluate how promoters in series affect each other and further characterize the dual state phenomenon. Here we used E. coli for the ease at which it is genetically manipulated to provide proof of principle. Additionally, we tested these promoters with GFP for an easy visual output. Ultimately the aim is to engineer bacteria native to the oral microbiome to sense and respond to drops in pH. We envision that these bacteria will induce enzymes that catalyze reactions to counteract the decrease in pH, preventing the demineralization of the tooth. </p> | ||
+ | |||
</div> | </div> | ||
Line 271: | Line 330: | ||
<div id = "problem"> | <div id = "problem"> | ||
<p id = "first-row-title"> Summary of Problem </p> | <p id = "first-row-title"> Summary of Problem </p> | ||
- | <center> <img src="https://static.igem.org/mediawiki/2013/thumb/ | + | <center> <a href = "https://2013.igem.org/Team:Northwestern/problem"> <img src="https://static.igem.org/mediawiki/2013/thumb/2/2c/Summary-of-problem.gif/600px-Summary-of-problem.gif" width="120" height="120"/> </a></center> |
<p id = "first-row-description"> Following meals, the pH within the mouth drops substantially which leads to cavities. </p> | <p id = "first-row-description"> Following meals, the pH within the mouth drops substantially which leads to cavities. </p> | ||
Line 277: | Line 336: | ||
<div id = "pH"> | <div id = "pH"> | ||
<p id = "first-row-title"> pH </p> | <p id = "first-row-title"> pH </p> | ||
- | <center> <img src="https://static.igem.org/mediawiki/2013/thumb/1/1e/Ph-image.gif/120px-Ph-image.gif" width="120" height="120"/></center> | + | <center> <a href = "https://2013.igem.org/Team:Northwestern/detectpH"><img src="https://static.igem.org/mediawiki/2013/thumb/1/1e/Ph-image.gif/120px-Ph-image.gif" width="120" height="120"/></center> |
<p id = "first-row-description"> The first step in combating cavities is to create a bacteria that can detect pH. </p> | <p id = "first-row-description"> The first step in combating cavities is to create a bacteria that can detect pH. </p> | ||
</div> | </div> | ||
<div id = "dual-state"> | <div id = "dual-state"> | ||
<p id = "first-row-title"> Dual State Promoter </p> | <p id = "first-row-title"> Dual State Promoter </p> | ||
- | <center> <img src="https://static.igem.org/mediawiki/2013/thumb/1/14/Dual-state-image.gif/ | + | <center> <a href = "https://2013.igem.org/Team:Northwestern/dualstate"><img src="https://static.igem.org/mediawiki/2013/thumb/1/14/Dual-state-image.gif/180px-Dual-state-image.gif" width="120" height="120"/></center> </a> |
<p id = "first-row-description"> In order for a quick response from basal level, we designed the dual state promoter. </p> | <p id = "first-row-description"> In order for a quick response from basal level, we designed the dual state promoter. </p> | ||
</div> | </div> |
Latest revision as of 04:04, 28 September 2013
NUtralize!
Poor oral health (tooth decay and infection) can lead to many painful symptoms and complicated treatments. Mouth bacteria are a primary culprit in tooth decay, as lactic acid secreted by natural bacteria in the human mouth decreases pH, leading to cavity formation. The enamel demineralization threshold is known to be around pH 5.5. The overall vision of our project is to prevent mouth pH from falling below 5.5 by engineering bacteria to sense pH and respond by increasing the alkalinity. To do this, we aimed to create genetic pH sensing elements that would work in the mouth.
Our project laid the foundation for a dual-state promoter consisting of two promoters in series: a constitutive promoter active at a (tunable) basal level and an upstream, pH-inducible promoter. Previous research has found that the E. coli genes Asr and GadA are highly expressed when pH drops below 5.5. This research drove us to choose the promoter elements for GadA and Asr as our pH-inducible promoters.
We have characterized a new pH-responsive promoter (Asr) as well as an iGEM-characterized promoter (GadA) and a constitutive promoter (Lpp). Ongoing work will evaluate how promoters in series affect each other and further characterize the dual state phenomenon. Here we used E. coli for the ease at which it is genetically manipulated to provide proof of principle. Additionally, we tested these promoters with GFP for an easy visual output. Ultimately the aim is to engineer bacteria native to the oral microbiome to sense and respond to drops in pH. We envision that these bacteria will induce enzymes that catalyze reactions to counteract the decrease in pH, preventing the demineralization of the tooth.