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Team:Imperial College/PHB production
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<h2 class="clear">Nile red staining</h2> | <h2 class="clear">Nile red staining</h2> | ||
| - | [[File:Nilered.JPG|thumbnail|right|400px|<b>left</b> control cells <b>right phaCAB transformed MG1655</b>]] <br><br><br> | + | {| class="wikitable" style="margin: 1em auto 1em auto;" |
| + | |[[File:Nilered.JPG|thumbnail|right|400px|<b>left</b> control cells <b>right phaCAB transformed MG1655</b>]] <br><br><br> | ||
O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB. | O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB. | ||
| - | + | |[[File:27-9-13phaCABall.jpg|thumbnail|right|400px|text]] | |
| - | + | |} | |
Revision as of 12:14, 28 September 2013
PHB production
Nile red staining
 O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB. |  |
Purification of P3HB
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