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Team:DTU-Denmark/Notebook/21 August 2013
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==Main purpose== | ==Main purpose== | ||
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| + | *USER reaction | ||
| + | *running gels | ||
==Who was in the lab== | ==Who was in the lab== | ||
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==Conclusion== | ==Conclusion== | ||
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| + | PCR: | ||
| + | *got pZA21::RFP:araBAD linearized and Sec2 | ||
| + | *the gradient PCR did not work | ||
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Latest revision as of 17:48, 28 September 2013
21 August 2013
Contents |
lab 208
Main purpose
- USER reaction
- running gels
Who was in the lab
Kristian, Henrike
Procedure
USER ligation and transformation
- with opened pZA21::ara and HAO, AMO, Cyc
- also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends
Results
Gels
- 1 kb ladder
- pZA21::RFP:araBAD linearized
- pZA21::RFP:araBAD linearized (duplicate)
- test PCR Sec2
- test PCR Sec2 (duplicate)
- screening sample A4
- screening sample A5
- screening sample A6
- screening sample A7
- screening sample A8
- screening sample A9
- screening sample A10
- screening sample A11
- screening sample A12
- screening sample B1
- screening sample B2
- screening sample B3
- screening sample B4
- 1 kb ladder
- 1 kb ladder
- screening sample B5
- screening sample B6
- screening sample B7
- screening sample B8
- screening sample B9
- screening sample B10
- screening sample B11
- screening sample B12
- screening sample C1
- screening sample C2
- screening sample C3
- screening sample C4
- screening sample C5
- 1 kb ladder
- 1 kb ladder
- screening sample C6
- screening sample C7
- screening sample C8
- screening sample C9
- screening sample C10
- screening sample C11
- screening sample C12
Conclusion
PCR:
- got pZA21::RFP:araBAD linearized and Sec2
- the gradient PCR did not work
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