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Team:Groningen/3 July 2013
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'''Mirjam and Sander''' | '''Mirjam and Sander''' | ||
| - | The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. | + | <br/>The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. |
| + | Turned out that the one with DMSO had better results. Did a gel purification (standard protocol). | ||
| + | |||
| + | '''Sander and Inne''' | ||
| + | <br/>Did a PCR for the silk template, in duplo. | ||
| + | With the following protocol: | ||
| + | <br>1 2 3 4 5 6 | ||
| + | <br>98 98 85 72 72 4 | ||
| + | <br>2:00 0:30 0:25 0:27 10:00 forever | ||
| + | |||
| + | So two with DMSO and two without. | ||
| + | NB: another change is that we used 5% DMSO this time | ||
| + | |||
| + | Ran a gel which had more bigger bands them the previous pcr. | ||
| + | The samples without DMSO were clearer than those with DMSO. | ||
Latest revision as of 08:42, 4 July 2013
Mirjam
Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.
Mirjam and Sander
The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO.
Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).
Sander and Inne
Did a PCR for the silk template, in duplo.
With the following protocol:
1 2 3 4 5 6
98 98 85 72 72 4
2:00 0:30 0:25 0:27 10:00 forever
So two with DMSO and two without. NB: another change is that we used 5% DMSO this time
Ran a gel which had more bigger bands them the previous pcr. The samples without DMSO were clearer than those with DMSO.
