Exeter/14 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "==MiniPrep== We Miniprepped the following parts: * Promoter and RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002]) * Magenta pigment ([http://part...")
Line 56: Line 56:
| KAN plasmid || 23.6 || 10.60 || 5.4
| KAN plasmid || 23.6 || 10.60 || 5.4
|}
|}
 +
 +
==Gel of digest==
 +
 +
{| class="wikitable"
 +
|-
 +
! Lane !! Part
 +
|-
 +
| 1 || empty (well looked damaged)
 +
|-
 +
| 2 || 1kb GeneRuler Ladde
 +
|-
 +
| 3 || Terminator
 +
|-
 +
| 4 || RFP control
 +
|-
 +
| 5 || skipped, looked damaged
 +
|-
 +
| 6 || skipped, looked damaged
 +
|-
 +
| 7 || Magenta pigment
 +
|-
 +
| 8 || KAN plasmid
 +
|-
 +
| 9 || Yellow pigment w/ RBS
 +
|-
 +
| 10 || Yellow pigment
 +
|-
 +
| 11 || 1kb GeneRuler Ladder
 +
|}
 +
 +
[[Image:14.8.13.jpg|center||500px|Image: 500 pixels]]

Revision as of 10:43, 29 September 2013

Contents

MiniPrep

We Miniprepped the following parts:

  • Promoter and RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K608002 BBa_K608002])
  • Magenta pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592012 BBa_K592012])
  • Yellow pigment ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K592010 BBa_K592010])
  • Yellow pigment with RBS ([http://parts.igem.org/wiki/index.php?title=Part:BBa_S05058 BBa_S05058])
  • Terminator ([http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015])
  • RFP control ([http://parts.igem.org/wiki/index.php?title=Part:BBa_J04450 BBa_KJ04450])

NanoDrop results

Part ng/ul
RFP control 80.7
Yellow w/ RBS 60.8
Promoter and RBS 127.8
Magenta pigment 46.9
Terminator 89.9
Yellow pigment 59.0
KAN plasmid 23.6

Digestion

Part ng/ul from NanoDrop ul need to have 250ng DNA 16ul - required ul DNA (amount of water needed for digestion)
RFP control 80.7 3.10 12.9
Yellow w/ RBS 60.8 4.11 11.89
Promoter and RBS 127.8 2.0 14.0
Magenta pigment 46.9 5.33 10.67
Terminator 89.9 2.80 13.2
Yellow pigment 59.0 4.24 11.76
KAN plasmid 23.6 10.60 5.4

Gel of digest

Lane Part
1 empty (well looked damaged)
2 1kb GeneRuler Ladde
3 Terminator
4 RFP control
5 skipped, looked damaged
6 skipped, looked damaged
7 Magenta pigment
8 KAN plasmid
9 Yellow pigment w/ RBS
10 Yellow pigment
11 1kb GeneRuler Ladder
Image: 500 pixels
Retrieved from "http://2013.igem.org/Exeter/14_August_2013"