Team:UNITN-Trento/Notebook/Labposts/07/78

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Revision as of 13:49, 29 September 2013

{ "date":"2013-07-06", "author":"viola", "title":"cloning of EFE in pSpac and pXyl vectors", "content":" Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1|

LIGATION	EFE+B0015 in pSPAC	EFE+B0015 in pXYL
PLASMID CONC.	20 µl	20 µl
PLASMID LENGHT	7082 BP	3594 BP
INSERT CONCENTRATION	20 µl	20 µl
INSERT LENGHT	1251 BP	1251 BP
PLASMID USED	50 ng/µl	50 ng/µl
REACTION VOLUME	20 µl	20 µl
BUFFER	10 µl	10 µl

}} }}

LIGATION TABLE PSPAC

EFE IN PSPAC	CTRL	1:1	1:2	1:4
INSERT (µl)	0	0.44	0.88	1.76
WATER (µl)	14.5	14.06	13.62	12.74
LIGASE (µl)	1	1	1	1
PLASMID (µl)	2.5	2.5	2.5	2.5
BUFFER (µl)	2	2	2	2

LIGATION TABLE PXYL

EFE IN PXYL	CTRL	1:1	1:2	1:4
INSERT (µl)	0	0.87	1.74	3.48
WATER (µl)	14.5	13.63	12.76	11.02
LIGASE (µl)	1	1	1	1
PLASMID (µl)	2.5	2.5	2.5	2.5
BUFFER (µl)	2	2	2	2

}} }} </html>", "tags":"BACILLUS" }

@@ Line 2: / Line 2: @@
 "date":"2013-07-06",
 "author":"viola",
-"title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>",
+"title":"<html>cloning of EFE in pSpac and pXyl vectors</html>",
 "content":"<html> Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1| <html> <center> <table> <tr> <th>LIGATION</th> <th>EFE+B0015 in pSPAC</th> <th>EFE+B0015 in pXYL</th> </tr> <tr> <th>PLASMID CONC.</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>PLASMID LENGHT</th> <td>7082 BP</td> <td>3594 BP</td> </tr> <tr> <th>INSERT CONCENTRATION</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>INSERT LENGHT</th> <td>1251 BP</td> <td>1251 BP</td> </tr> <tr> <th>PLASMID USED</th> <td>50 ng/µl</td> <td>50 ng/µl</td> </tr> <tr> <th>REACTION VOLUME</th> <td>20 µl</td> <td>20 µl</td> </tr> <tr> <th>BUFFER</th> <td>10 µl</td> <td>10 µl</td> </tr> </table> </center> </html>}} <html> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PSPAC| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PSPAC</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.44</td> <td>0.88</td> <td>1.76</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>14.06</td> <td>13.62</td> <td>12.74</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE PXYL| <html> <center> <table> <tr> <th><font color=\"red\">EFE IN PXYL</font></th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>INSERT (µl)</th> <td>0</td> <td>0.87</td> <td>1.74</td> <td>3.48</td> </tr> <tr> <th>WATER (µl)</th> <td>14.5 </td> <td>13.63</td> <td>12.76</td> <td>11.02</td> </tr> <tr> <th>LIGASE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <th>BUFFER (µl)</th> <td>2</td> <td>2</td> <td>2</td> <td>2</td> </tr> </table> </center> </html>}} <html> </html>}} <html> </html>}} </html>",
 "tags":"BACILLUS"
 }