Exeter/8 August 2013

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== Liquid Cultures, Miniprepped ==
== Liquid Cultures, Miniprepped ==
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2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.
2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Revision as of 07:23, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

Liquid Cultures, Miniprepped

  • K592012 (Magenta) CAM
  • RFP control CAM
  • R0082 (OmpF promoter) CAM
  • DH5α 3 (Tom and Adam's control) TET
  • DH5α 5 (Tom and Adam's RBS + Cyan) TET
  • DH5α 1 (Tom and Adam's RBS + cph8) TET
  • S05058 (RBS + yellow) CAM
  • K592010 (yellow) CAM
  • K592018 (RBS + cph8) CAM
  • K608002 (RBS + promoter) CAM
  • B0015 (Terminator) CAM
  • K322124 (RBS + cph8) CAM

( 3 replicates, #2, #3 and #4.)


NanoDropping Promoter + RBS Replicates

1 = 29.6ng/ul

1 = 29.2ng/ul

2 = 12.2ng/ul

2 = 12.5ng/ul

3 = 22.8ng/ul

3 = 23.6ng/ul

4 = 17.8ng/ul

4 = 17.5ng/ul


NanoDrop of liquid cultures

Liquid culture NanoDrop result (ng/ul)
Magenta 106.9
RFP 42.9
OmpF 117.3
DH5α 3 46.3
S05058 67.1
Yellow 57.1
RBS + cph8 113.7
cph8 #4 49.2 and 48.8
cph8 #2 221.9 and 180.7
cph8 #3 70.8 and 70.8
Promoter and RBS 99.9
B0015 52.1
AMP plasmid 82.5


We sent off cph8 K322124 red light sensor for sequencing.


2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/8_August_2013"