Team:EPF Lausanne/Calendar/19 August 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Gel electrophoresis of the backbones with and without GFP''' <BR> After running the gel for 1.5h, there were two visible bands for t...")
Line 8: Line 8:
'''Gradient PCR of inserts''' <BR>
'''Gradient PCR of inserts''' <BR>
As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C.
As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C.
 +
This Gradient PCR worked very well, the inserts were amplified at almost all of the temperatures.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 17:59, 30 September 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Gel electrophoresis of the backbones with and without GFP
After running the gel for 1.5h, there were two visible bands for the backbones but not at the right height. We were not sure if there was a problem with the ladder.

Gradient PCR of inserts
As we were not sure about the annealing temperatures of the primers for the MMP inserts, we ran a gradient PCR for the MMP2 and MMP9 inserts with 8 different temperatures between 56°C and 65°C. This Gradient PCR worked very well, the inserts were amplified at almost all of the temperatures.


Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/19_August_2013"