Team:UTK-Knoxville/11 June 2013
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<h3> <span class="mw-headline" id="Tuessday_11_June_13"> Tuesday 11 June 13 </span> </h3> | <h3> <span class="mw-headline" id="Tuessday_11_June_13"> Tuesday 11 June 13 </span> </h3> | ||
+ | <p>Brandon re-plated transformations from yesterday and they did not work. Brandon will leave them in the incubator a little longer to watch for growth, but does not expect any. | ||
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+ | Still waiting to hear back from Eric on the status of the ligation/transformation he did. Last Friday (6/7) he picked up digested backbone, Aer-EnvZ, and OmpR, and planned to ligate the pieces and transform them in time to allow them to grow before today’s 5:00 meeting. | ||
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+ | Eric was able to confirm our Aer-EnvZ+OmpR construct in his lab. It has been confirmed in wildtype E. Coli. We need to prepare chemically competent ΔOmpR, ΔEnvZ, and ΔOmpR+ΔEnvZ transform our plasmid into.</p> | ||
<li> Whole group meeting </li> | <li> Whole group meeting </li> | ||
<li> Aer/EnvZ is in the wild type.</li> | <li> Aer/EnvZ is in the wild type.</li> |