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| - | <h1>Backbone</h1>
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| - | <p>
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| - | To transform <i>B. subtilis</i> with our developed biobricks we have improved the <i>amyE</i> intergrational backbone from Munich's iGEM team 2012 (<a href="http://parts.igem.org/Part:BBa_K823023">BBa_K823023</a>) that had no inducible promoter. We've added the HyperSpank IPTG inducible promoter from Rudner's Lab 2004. This promoter is placed right in front of the prefix, so any biobrick can be inserted in this backbone (<a href="http://parts.igem.org/Part:BBa_K1085014">BBa_K1085014</a>)and can be induced with IPTG.<p>
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| - | In figure 1 a close-up of the promoter is shown. Two lacO operaters
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| - | <table id="layout" width=60%> <!--change the percentage to determine the size of your image-->
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| - | <img src="https://static.igem.org/mediawiki/2013/archive/d/dc/20130912093105%21Hyspank_in_BBa_K1085014.jpg" width="100%"> <!--only insert the link, do not change the percentage!-->
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| - | <font size="1">Figure 1: Hy_Spank promoter</font>
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| - | <table id="layout" width=40%> <!--change the percentage to determine the size of your image-->
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| - | <font size="1">Figure 2: </font>
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| - | <h2>Promoter activity</h2><br>
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| - | <table id="layout" width=60%> <!--change the percentage to determine the size of your image-->
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| - | <img src="https://static.igem.org/mediawiki/2013/9/9e/Analysis%2Bpictures.jpg" width="100%"></img>
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| - | <font size="1">Figure 3: The intensity in of ~40 cells in the GFP-channel, were analyzed from two pictures.
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| - | <br>The average intensity (AU) from the cells are plotted above with the standard deviation.<Br></font>
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| - | <h2>GFP<sub>mg</sub> and GFP (BBa_E0840)</h2>
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| - | <table id="layout" width=45%> <!--change the percentage to determine the size of your image-->
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| - | <img src="https://static.igem.org/mediawiki/igem.org/1/16/GFPdsm_%2BGFP840.jpg" width="100%"></img>
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| - | <font size="1">Figure 4: <Br></font>
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