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Team:IIT Madras/Notebook
From 2013.igem.org
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(a) Added 1-2µl of DNA to competent cell. | (a) Added 1-2µl of DNA to competent cell. | ||
| - | |(b) Kept on ice for 30 minutes. | + | |-(b) Kept on ice for 30 minutes. |
|(c) Heat Shock at 42°C for 90 sec. | |(c) Heat Shock at 42°C for 90 sec. | ||
|(d) K | |(d) K | ||
Revision as of 18:32, 6 July 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Phase I : Ideation
Phase II : Experimentation
e-Notebook
| May 13 | June 13 | July 13 | ||||||||||||||||||||||||||||||||
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| S | M | T | W | T | F | S | S | M | T | W | T | F | S | S | M | T | W | T | F | S | ||||||||||||||
| 1 | 2 | 3 | 4 | 1 | 1 | 2 | 3 | 4 | 5 | 6 | ||||||||||||||||||||||||
| 5 | 6 | 7 | 8 | 9 | 10 | 11 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | ||||||||||||||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | ||||||||||||||
| 13 | 14 | 15 | 16 | 17 | 18 | 19 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | ||||||||||||||
| 20 | 21 | 22 | 23 | 24 | 25 | 26 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 28 | 29 | 30 | 31 | |||||||||||||||||
| 27 | 28 | 29 | 30 | 31 | 30 | |||||||||||||||||||||||||||||
Experimental results
Protocols
1. Transformation:
(a) Added 1-2µl of DNA to competent cell. |-(b) Kept on ice for 30 minutes. |(c) Heat Shock at 42°C for 90 sec. |(d) K
Phase III
Troubleshooting
Discussions
This page is under construction. For more information contact Rohan or Aman.
