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Team:DTU-Denmark/Methods
From 2013.igem.org
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Latest revision as of 12:31, 1 October 2013
Methods
Contents |
PCR
USER Cloning
USER Cloning and Transformation
Plasmid isolation
Plasmid isolation by ethanol precipitation
Determining concentration of nitrogen compounds
Experiment 1a -- Toxicity of Nitrite, Nitrate and Ammonium to E. coli
Experiment 2 -- Anaerobic production of nitrous oxide from nitrite
Experiment 4 -- Aerobic consumption of ammonium and hydroxylamine
Other
Visualizing GFP in the periplasm
Preparation of chemically competent cells
Ammonium colorimetric measurements
Nitrate colorimetric measurements
Nitrite colorimetric measurements
